| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44218 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP2-30108AFP405 | |||
| Alexa Fluor Plus 488 | NBP2-30108AFP488 | |||
| Alexa Fluor Plus 555 | NBP2-30108AFP555 | |||
| Alexa Fluor Plus 594 | NBP2-30108AFP594 | |||
| Alexa Fluor Plus 647 | NBP2-30108AFP647 | |||
| Alexa Fluor Plus 680 | NBP2-30108AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30108SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56102SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44782 | |||
| Azide and BSA Free | NBP2-34515-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB810-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44778 | |||
| Azide and BSA Free | NBP2-34440-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34442-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75764 | |||
![Western Blot: Bcl-2 Antibody (CL7935) [NBP2-88910] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-CL7935-Western-Blot-NBP2-88910-img0008.jpg "Western Blot: Bcl-2 Antibody (CL7935) [NBP2-88910] - Analysis in human cell line HDLM-2.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Bcl-2 Antibody (CL7935) [NBP2-88910] - Analysis in human thyroid gland and liver tissues using NBP2-88910 antibody. Corresponding BCL2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-CL7935-Immunohistochemistry-Paraffin-NBP2-88910-img0007.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (CL7935) [NBP2-88910] - Staining of human breast cancer shows moderate positivity.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-CL7935-Immunohistochemistry-Paraffin-NBP2-88910-img0001.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (CL7935) [NBP2-88910] - Staining of human liver shows negative to no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-CL7935-Immunohistochemistry-Paraffin-NBP2-88910-img0002.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (CL7935) [NBP2-88910] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-CL7935-Immunohistochemistry-Paraffin-NBP2-88910-img0003.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (CL7935) [NBP2-88910] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-CL7935-Immunohistochemistry-Paraffin-NBP2-88910-img0004.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (CL7935) [NBP2-88910] - Staining of human rectum shows strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-CL7935-Immunohistochemistry-Paraffin-NBP2-88910-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (CL7935) [NBP2-88910] - Staining of human thyroid gland shows strong positivity in cytoplasm granular in glandular cells.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-CL7935-Immunohistochemistry-Paraffin-NBP2-88910-img0006.jpg)
![Western Blot: Bcl-2 Antibody [NBP2-30108] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), Bcl-2 from Company B, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NBP2-30108-img0011.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Bcl-2 Antibody [NBP2-30108] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), Bcl-2 from Company B, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 7 Immunofluorescence Validation of Bcl-2 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling Bcl-2 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).](http://images.novusbio.com/fullsize/nbp2-30108_rabbit-polyclonal-bcl-2-antibody-271120242045654.gif)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP2-30108] - Immunohistochemistry Validation of Bcl-2 in Human Kidney. Immunohistochemical analysis of paraffin-embedded Human Kidney using anti-Bcl-2 antibody (NBP2-30108) at 2ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NBP2-30108-img0012.jpg)
![Western Blot: Bcl-2 Antibody [NBP2-30108] - Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Human Daudi cellsLane B: Mouse A-20 cells](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NBP2-30108-img0009.jpg)
![Western Blot: Bcl-2 Antibody [NBP2-30108] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Western-Blot-NBP2-30108-img0010.jpg)
![Immunohistochemistry: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 6 Immunohistochemistry Validation of Bcl-2 in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-Bcl-2 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-30108_rabbit-polyclonal-bcl-2-antibody-271120241937773.gif)
![Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Validation of Bcl-2 in Human Kidney Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Kidney Cells labeling Bcl-2 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp2-30108_rabbit-polyclonal-bcl-2-antibody-271120242045666.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56102] - Formalin-fixed paraffin-embedded section of a human prostate cancer tissue array stained for Bcl-2 expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56102-img0003.jpg "Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56102] - Formalin-fixed paraffin-embedded section of a human prostate cancer tissue array stained for Bcl-2 expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.")
![Western Blot: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Western Blot Analysis of MCF-7 cell lysate using Bcl-2 Antibody (100/D5+124).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5+124-Western-Blot-NBP2-34444-img0005.jpg "Western Blot: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Western Blot Analysis of MCF-7 cell lysate using Bcl-2 Antibody (100/D5+124).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with Bcl-2 MAb (100/D5 + 124). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5+124-Immunohistochemistry-Paraffin-NBP2-34444-img0001.jpg)
![Flow Cytometry: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Intracellular staining of Bcl-2 in Jurkat cells using Bcl-2 MAb (100/D5 + 124) (red) and isotype control (green).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5+124-Flow-Cytometry-NBP2-34444-img0002.jpg)
![Western Blot: Bcl-2 Antibody (100/D5+124) [NBP2-34444] - Western blot analysis of Bcl-2 in human skin using Bcl-2 Ab (100/D5 + 124).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-100-D5+124-Western-Blot-NBP2-34444-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of NIH/3T3 cells using Bcl2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8E12-Immunocytochemistry-Immunofluorescence-NBP2-22170-img0002.jpg "Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of NIH/3T3 cells using Bcl2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of colon cancer tissues (left) and human brain tissues (right) using Bcl2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8E12-Immunohistochemistry-Paraffin-NBP2-22170-img0003.jpg)
![Flow Cytometry: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of 3T3L1 cells using Bcl2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-8E12-Flow-Cytometry-NBP2-22170-img0001.jpg)
![Western Blot: Bcl-2 Antibody (SPM117) [NBP2-34391] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (SPM117).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM117-Western-Blot-NBP2-34391-img0004.jpg "Western Blot: Bcl-2 Antibody (SPM117) [NBP2-34391] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (SPM117).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (SPM117) [NBP2-34391] - Formalin-fixed, paraffin-embedded human tonsil stained with Bcl-2 Mouse Monoclonal Antibody (SPM117).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM117-Immunohistochemistry-Paraffin-NBP2-34391-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (SPM117) [NBP2-34391] - Formalin-paraffin non-Hodgkin's lymphomastained with Bcl-2 Ab (SPM117). Note nuclear membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM117-Immunohistochemistry-Paraffin-NBP2-34391-img0001.jpg)
![Western Blot: Bcl-2 Antibody (SPM530) [NBP2-34389] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (SPM530).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM530-Western-Blot-NBP2-34389-img0004.jpg "Western Blot: Bcl-2 Antibody (SPM530) [NBP2-34389] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (SPM530).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (SPM530) [NBP2-34389] - Formalin-fixed, paraffin-embedded tonsil stained with Bcl-2 Mouse Monoclonal Antibody (SPM530).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM530-Immunohistochemistry-Paraffin-NBP2-34389-img0005.jpg)
![Western Blot: Bcl-2 Antibody (BCL2/2210R) [NBP2-75761] - Analysis of human MCF-7 cell lysate using Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/2210R).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-2210R-Western-Blot-NBP2-75761-img0003.jpg "Western Blot: Bcl-2 Antibody (BCL2/2210R) [NBP2-75761] - Analysis of human MCF-7 cell lysate using Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/2210R).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/2210R) [NBP2-75761] - Formalin-fixed, paraffin-embedded human Follicular Lymphoma stained with Bcl-2 Antibody (BCL2/2210R).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-2210R-Immunohistochemistry-Paraffin-NBP2-75761-img0004.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (BCL2/2210R) [NBP2-75761] - Formalin-fixed, paraffin-embedded human spleen stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/2210R).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-2210R-Immunohistochemistry-Paraffin-NBP2-75761-img0001.jpg)
![Protein Array: Bcl-2 Antibody (BCL2/2210R) [NBP2-75761] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Bcl-2 Antibody (BCL2/2210R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-BCL2-2210R-Protein-Array-NBP2-75761-img0005.jpg)
![Immunohistochemistry: Bcl-2 Antibody (PD01-41) [NBP3-32073] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32073) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32073_rabbit-bcl-2-mab-pd01-41-29820241430412.jpg "Immunohistochemistry: Bcl-2 Antibody (PD01-41) [NBP3-32073] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32073) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody (PD01-41) [NBP3-32073] - Immunocytochemistry analysis of HeLa cells labeling Bcl-2 with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32073_rabbit-bcl-2-mab-pd01-41-29202410234278.jpg)
![Western Blot: Bcl-2 Antibody (PD01-41) [NBP3-32073] - Western blot analysis of Bcl-2 on different lysates with Rabbit anti-Bcl-2 antibody (NBP3-32073) at 1/2,000 dilution.Lane 1: MCF7 cell lysate (15 µg/Lane)Lane 2: Jurkat cell lysate (15 µg/Lane)Lane 3: HL-60 cell lysate (15 µg/Lane)Lane 4: THP-1 cell lysate (15 µg/Lane)Predicted band size: 26 kDaObserved band size: 25 kDaExposure time: 1 minute 30 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32073_rabbit-bcl-2-mab-pd01-41-3920248485017.jpg)
![ELISA: Mouse Bcl-2 ELISA Kit (Colorimetric) [NBP2-69946] - Samples were spiked with high concentrations of Mouse Bcl-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69946_mouse-bcl-2-elisa-kit-colorimetric-132202416221063.jpg "ELISA: Mouse Bcl-2 ELISA Kit (Colorimetric) [NBP2-69946] - Samples were spiked with high concentrations of Mouse Bcl-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Bcl-2 ELISA Kit (Colorimetric) [NBP2-69946] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69946_mouse-bcl-2-elisa-kit-colorimetric-4320248582563.jpg)