| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85500-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB37401-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3740-SP | |||
| Carrier Free | FAB3740V-100UG | |||
| Carrier Free | FAB3740U-100UG | |||
| Carrier Free | FAB3740T-100UG | |||
| Carrier Free | FAB3740S-100UG | |||
| Carrier Free | FAB3740R-100UG | |||
| Carrier Free | FAB3740N-100UG | |||
| Carrier Free | FAB3740G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05599-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26362-50ul | |||
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: COX-1 Antibody [NBP1-85500] - Analysis in human cell line HELA.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NBP1-85500-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody [NBP1-85500] - Staining of human cell line BJ shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85500-img0009.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0010.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0011.jpg)
![Simple Western: COX-1 Antibody [NBP1-85500] - Lane view shows one specific signal (~70kDa) in lysates prepared from the mouse tail vein. Image from a verified customer review.](http://images.novusbio.com/fullsize/COX-1-Antibody-Simple-Western-NBP1-85500-img0013.jpg)
![Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NB100-867-img0010.jpg "Western Blot: COX-1 Antibody [NB100-867] - Staining (1ug/ml) of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: COX-1 Antibody [NB100-867] - Staining (3.8µg/ml) of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-Staining.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NB100-867-img0009.jpg)
![Immunohistochemistry: COX-1 Antibody [NB100-867] - Immunohistochemistry and Virtual Channel confocal microscopy of IL-4 and IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) and PTGS1/COX-1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-020-65469-0), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-NB100-867-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Intestinal tuft cells express advillin. (a) Immunohistochemistry of paraffin-embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & DCLK1 (red) antibodies. Nuclei are counter stained with DAPI. Both proteins co-localize in the same cell. Note that not all DCLK1 positive cells are positive for advillin expression. Right panel shows higher magnification of boxed area & shows co-localization of DCLK1 & advillin in a tuft cell. (b) Immunohistochemistry of paraffin embedded tissue from distal ileum of C57BL/6 J mice using advillin (green) & PTGS1 (red) antibodies. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of PTGS1 & advillin in a tuft cell. (c) Immunohistochemistry of cryopreserved tissue from distal ileum of C57BL/6 J mice using Alexa Fluor 568 Phalloidin (red) & advillin (green). Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area & co-localization of advillin & F-actin at the apical surface of a tuft cell. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628497.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - In intestinal enteroids, tuft cells express advillin. (a) Immuno-histochemistry of enteroids from distal ileum of C57BL/6 J mice shows tuft cell hyperplasia 72 hours post IL-4 & IL-13 treatment. Control refers to untreated enteroids from C57BL/6 J mice. Advillin (green) & DCLK1 (red) co-localization was used to identify tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of the boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization in tuft cells of advillin (green) & DCLK1 (red) in the upper panel; & advillin & PTGS1 (red) in the lower panel. Nuclei were counter stained with DAPI. Right panels show higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-31020241628492.jpg)
![Immunocytochemistry/ Immunofluorescence: COX-1 Antibody [NB100-867] - Tuft cells positive for advillin are negative for villin. (a) Immunohistochemistry & Virtual Channel confocal microscopy of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice, show co-localization of advillin (green), DCLK1 (red) & PTGS1 (magenta) within the same cells identified as tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. (b) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show co-localization in tuft cells of advillin (green) with F-actin (red) in the upper panel; & co-localization of advillin (red) & tubulin (green) in the lower panel. Nuclei were counter stained with DAPI. Right panel shows higher magnification of boxed area. Note the co-localization of advillin to the apical tuft that terminates near the perinuclear region of the cells. Advillin appears in vesiculated structures that co-localize with tubulin near the lateral cell surface & in the cytoplasm. (c) Immunohistochemistry of IL-4 & IL-13 treated enteroids from distal ileum of C57BL/6 J mice show the absence of villin (red) from advillin (green) expressing tuft cells. Nuclei are counter stained with DAPI. Right panel shows higher magnification of boxed area. Data shown are representative of n = 5 animals. Scale bar represents 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483224), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-867_goat-polyclonal-cox-1-antibody-310202416284923.jpg)
![Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX-1 on different lysates with Rabbit anti-COX-1 antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Western-Blot-NBP2-67643-img0011.jpg "Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX-1 on different lysates with Rabbit anti-COX-1 antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 69 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0010.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-COX-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0012.jpg)
![Flow Cytometry: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of Hela cells with COX1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Flow-Cytometry-NBP2-67643-img0001.jpg)
![Western Blot: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of COX1 on different lysates using anti-COX1 antibody at 1/1,000 dilution. Positive control: Lane 1: C2C12 Lane 2: A431](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Western-Blot-NBP2-67643-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (SC68-05) [NBP2-67643] - Staining COX1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunocytochemistry-Immunofluorescence-NBP2-67643-img0009.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded human breast cancer tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0004.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse brain tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0005.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse skin tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0006.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody (SC68-05) [NBP2-67643] - Analysis of paraffin-embedded mouse stomach tissue using anti-COX1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/COX-1-Antibody-SC68-05-Immunohistochemistry-Paraffin-NBP2-67643-img0007.jpg)
![Western Blot: COX-1 Antibody (S02-6G4) [NBP3-19609] - Western blot detection of COX-1 in C6, 3T3 cell lysates using NBP3-19609 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 69kDa.](http://images.novusbio.com/fullsize/COX-1-Antibody-S02-6G4-Western-Blot-NBP3-19609-img0003.jpg "Western Blot: COX-1 Antibody (S02-6G4) [NBP3-19609] - Western blot detection of COX-1 in C6, 3T3 cell lysates using NBP3-19609 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 69kDa.")
![Immunohistochemistry-Paraffin: COX-1 Antibody (S02-6G4) [NBP3-19609] - Immunohistochemistry of COX-1 in paraffin-embedded Human colon cancer tissue using NBP3-19609 at dilution 1/5](http://images.novusbio.com/fullsize/COX-1-Antibody-S02-6G4-Immunohistochemistry-Paraffin-NBP3-19609-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody (S02-6G4) [NBP3-19609] - Immunofluorescence of COX-1 (green) in Hela cells using NBP3-19609 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19609_rabbit-cox-1-mab-s02-6g4-1132025155597.jpg)
![Western Blot: COX-1 Antibody [NBP3-05599] - Western blot analysis of extracts of mouse skeletal muscle, using COX-1 antibody (NBP3-05599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NBP3-05599-img0002.jpg "Western Blot: COX-1 Antibody [NBP3-05599] - Western blot analysis of extracts of mouse skeletal muscle, using COX-1 antibody (NBP3-05599) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: COX-1 Antibody - Azide and BSA Free [COX-1] - Immunohistochemistry analysis of paraffin-embedded Human liver using COX-1 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-05599_rabbit-polyclonal-cox-1-antibody-5220251640246.jpg)
![Western Blot: COX-1 Antibody - Azide and BSA Free [COX-1] - Western blot analysis of lysates from NIH/3T3 cells using COX-1 Rabbit pAb at 1:1800 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.](http://images.novusbio.com/fullsize/nbp3-05599_rabbit-polyclonal-cox-1-antibody-5220251640224.jpg)
![Western Blot: COX-1 Antibody - Azide and BSA Free [COX-1] - Western blot analysis of lysates from NIH/3T3 cells, using COX-1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-05599_rabbit-polyclonal-cox-1-antibody-5220251638087.jpg)
![Western Blot: COX-1 Antibody (6B6) [NBP3-26362] - Positive Western Blot detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue All lanes: COX-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69, 65, 62, 57, 72, 73 kDa Observed band size: 72 kDa](http://images.novusbio.com/fullsize/nbp3-26362_rabbit-cox-1-mab-6b6-282202411472131.jpg "Western Blot: COX-1 Antibody (6B6) [NBP3-26362] - Positive Western Blot detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue All lanes: COX-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 69, 65, 62, 57, 72, 73 kDa Observed band size: 72 kDa")
![Flow Cytometry: COX-1 Antibody (6B6) [NBP3-26362] - Overlay histogram showing Hela cells stained with COX-1 Antibody (6B6) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26362_rabbit-cox-1-mab-6b6-28220241418366.jpg)
![Western Blot: COX1 Antibody [NBP1-62466] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/COX1-Antibody-Western-Blot-NBP1-62466-img0002.jpg "Western Blot: COX1 Antibody [NBP1-62466] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: COX-1 Antibody (8L4X10) [NBP3-16214] - Western blot analysis of extracts of various cell lines, using COX-1 Rabbit mAb (NBP3-16214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/COX-1-Antibody-8L4X10-Western-Blot-NBP3-16214-img0001.jpg "Western Blot: COX-1 Antibody (8L4X10) [NBP3-16214] - Western blot analysis of extracts of various cell lines, using COX-1 Rabbit mAb (NBP3-16214) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: COX1 Overexpression Lysate (Adult Normal) [NBL1-14943] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COX1.](http://images.novusbio.com/fullsize/COX1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14943-img0002.jpg "Western Blot: COX1 Overexpression Lysate (Adult Normal) [NBL1-14943] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COX1.")