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Products Related to Gene-ID: 54210

27 Results for "54210"

NBP3-42860

Porcine TREM1 ELISA Kit (Colorimetric)

1 Kit

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

Applications:

Standard Curve:

31.2 - 2000 pg/mL (example only; lot dependent)

Sensitivity:

13.1 pg/mL (example only; lot dependent)

Sample Type:

Serum, Plasma, Tissue homogenatesand other biological fluids

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MAB1187

TREM1 Antibody (174031) [Unconjugated]

(16 Publications)

100 μg

MAB1187

Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM‑1 Monoclonal Antibody (Catalog # MAB1187) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

<b>Biological Strategies Validation. </b>TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS using Rat Anti-Mouse TREM-1 Monoclonal Antibody (Catalog # MAB1187) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

ExNef potentiate inflammatory signalling cascade via re-localization of TLR4 & TREM-1 to lipid rafts.A–The effect of exNef (0.4 ng/ml, 48h) on abundance of lipid rafts & re-localization of TLR4 & TREM-1 to the plasma membrane in RAW264.7 macrophages. Left column, CTB staining; second column, anti-TREM-1 staining; third column anti-TLR4 staining; fourth column–merge TREM-1/rafts, right column–merge TLR4/rafts. Scale bars 10 μm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31344124), licensed under a CC-BY license. Not internally tested by R&D Systems.

TREM-1 activation induced HIF-1 alpha accumulation and translocation to the nucleus in macrophages. Macrophages were incubated with anti-TREM-1 mAb (10 μg/mL) in a normoxia condition. (A) Six hours later, Hif‐1 alpha gene levels were measured using qPCR, n=4-6. (B-C) Twenty-four hours later, the protein of HIF‐1 alpha was performed by western blot with GAPDH as a loading control, n=3. (D) Macrophages were cultured on anti-TREM-1 for 24 h and then subjected to immunofluorescence examination to analyze the HIF-1 alpha accumulation and translocation to the nucleus (scale bar, 50 μm). (E) Average fluorescent intensity was calculated by HIF-1 alpha + fluorescence intensity (IntDen)/area of the region (Area) using ImageJ, n=6. (F-G) 1×106 cells/well were premixed with PBS control or PX-478 (25 μM) for 30 min, then plated into 12-well plates with agonist anti-TREM-1 mAb (10 μg/mL). HK2 protein levels were measured after an additional incubation for 24 h, n=3. n represents experiments performed on different macrophages from separate mice. Data are expressed as the mean ± SD. Student's t-test (two-tailed, unpaired) was used to compare Mab1187 and Control in (A-E): * P < 0.05, ** P < 0.01, and *** P < 0.001. One-way ANOVA adjusted by Tukey's multiple comparison test was used in G: *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36594089), licensed under a CC-BY license. Not internally tested by R&D Systems.

TREM-1 activation induced HIF-1 alpha accumulation and translocation to the nucleus in macrophages. Macrophages were incubated with anti-TREM-1 mAb (10 μg/mL) in a normoxia condition. (A) Six hours later, Hif‐1 alpha gene levels were measured using qPCR, n=4-6. (B-C) Twenty-four hours later, the protein of HIF‐1 alpha was performed by western blot with GAPDH as a loading control, n=3. (D) Macrophages were cultured on anti-TREM-1 for 24 h and then subjected to immunofluorescence examination to analyze the HIF-1 alpha accumulation and translocation to the nucleus (scale bar, 50 μm). (E) Average fluorescent intensity was calculated by HIF-1 alpha + fluorescence intensity (IntDen)/area of the region (Area) using ImageJ, n=6. (F-G) 1×106 cells/well were premixed with PBS control or PX-478 (25 μM) for 30 min, then plated into 12-well plates with agonist anti-TREM-1 mAb (10 μg/mL). HK2 protein levels were measured after an additional incubation for 24 h, n=3. n represents experiments performed on different macrophages from separate mice. Data are expressed as the mean ± SD. Student's t-test (two-tailed, unpaired) was used to compare Mab1187 and Control in (A-E): * P < 0.05, ** P < 0.01, and *** P < 0.001. One-way ANOVA adjusted by Tukey's multiple comparison test was used in G: *** P < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36594089), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1187U-100UG
Alexa Fluor 405	FAB1187V-100UG
Alexa Fluor 488	FAB1187G-100UG
Alexa Fluor 532	FAB1187X
Alexa Fluor 594	FAB1187T-100UG
Alexa Fluor 647	FAB1187R-100UG
Alexa Fluor 700	FAB1187N-100UG
Alexa Fluor 750	FAB1187S-100UG
Allophycocyanin	FAB1187A
Allophycocyanin/Cy7	FAB1187APCCY7
Biotin	BAM1187
CoraFluor 1	FAB1187CL1
DyLight 350	FAB1187D
DyLight 405	FAB1187E
DyLight 488	FAB1187K
DyLight 550	FAB1187L
DyLight 594	FAB1187M
DyLight 650	FAB1187W
DyLight 680	FAB1187Y
DyLight 755	FAB1187Z
FITC	FAB1187F
HRP	FAB1187H
Janelia Fluor 525	FAB1187JF525
Janelia Fluor 549	FAB1187I
Janelia Fluor 646	FAB1187J
Janelia Fluor 669	FAB1187JF669
PE/Atto594	FAB1187PEATT594
PE/Cy5.5	FAB1187PECY55
PE/Cy7	FAB1187PECY7
Phycoerythrin	FAB1187P
mFluor Violet 450 SE	FAB1187MFV450
mFluor Violet 500 SE	FAB1187MFV500
mFluor Violet 610 SE	FAB1187MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1187P
Carrier Free	FAB1187A
Carrier Free	MAB1187-SP
Carrier Free	MAB1187-500
Carrier Free	FAB1187V-100UG
Carrier Free	FAB1187U-100UG
Carrier Free	FAB1187T-100UG
Carrier Free	FAB1187S-100UG
Carrier Free	FAB1187R-100UG
Carrier Free	FAB1187N-100UG
Carrier Free	FAB1187G-100UG

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QC210

Quantikine Immunoassay Control Set 867 Human TREM-1

1 Each

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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