Endomucin/Mucin -14: Novus Biologicals

AF4666

Endomucin/Mucin -14 Antibody [Unconjugated]

100 μg

AF4666

HAF017). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse kidney tissue, mouse lung tissue, and mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endomucin at approximately 75-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Endomucin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # <a class=

NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_lightbox">

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4666, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_thumb" />

MLK2/3-deficient mice exhibit defects in native collateral artery formation.(A) Representative confocal images (n = 5 mice) of control and MLK2/3-deficient whole mount P6 adductor muscle vasculature stained with antibodies to endomucin (capillary and venous vasculature, red) and SMA (arterial and venous smooth muscle, green). Gracilis collateral arteries in WT mice, but not MLK2/3-deficient mice, interconnect the PCFA to the SA. (B) Representative stereomicroscope images of P6 whole mount abdominal muscle stained with an antibody to SMA (green). Arteriole-to-arteriole arcades are indicated (red arrows). The abdominal muscle vasculature of Map3k10-/-Map3k11-/- mice shows very few arteriole-to-arteriole interconnections. Quantitation reveals significantly reduced arteriolar arcade numbers in Map3k10-/-Map3k11-/- mice compared to WT mice (mean ± SEM; n = 5 mice). Source data are included as Figure 3—source data 1.DOI://dx.doi.org/10.7554/eLife.18414.02310.7554/eLife.18414.024Figure 3—source data 1.Source data for Figure 3.This file contains raw source data used to make the graphs presented in Figure 3.DOI://dx.doi.org/10.7554/eLife.18414.024Source data for Figure 3.This file contains raw source data used to make the graphs presented in Figure 3.DOI://dx.doi.org/10.7554/eLife.18414.024 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27504807), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b> NOX2 contributes to the oxidative stress of bone ECs and the damage of angiogenesis-osteogenesis coupling. (A) qPCR analysis of the relative mRNA levels of three critical NOXs, involved in endothelial oxidative injuries, in the ECs in tibia metaphysis. T1DM mice were intraperitoneally injected with GKT137831 (NOX1/4 inhibitor) or one of the two NOX2 inhibitors, NOX2ds-tat (NOX2ds) and GSK2795039 (GSK), or VAS2870 (VAS, a pan-NOX inhibitor) once every two days from week 4 through 8. The levels of blood glucose (B), insulin (C) and body weight (D) during treatments were tested. (E) Confocal images of immunostained tibia sections show the CD31+Emcn+ type H vessels in the metaphysis at week 8. Scale bar: 50 μm on the above and 20 μm on the below. (F and G) Histomorphometric quantitation of the vessels in proximal tibia metaphysis. Flow cytometry quantification of type H ECs and total ECs in 8-week-old tibia (H). (I) Flow cytometry analysis of intracellular DCF fluorescence shows the ROS levels in the ECs in 8-week-old tibia. (B)-(D), n = 15-16 per group; other panels, n = 7-8 per group. ###p < 0.001 vs. Control group; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. DM+veh group. One-way or two-way ANOVA followed by Tukey posttest analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33664862), licensed under a CC-BY license. Not internally tested by R&D Systems.

$<b>Biological Strategies Validation. </b> The effects of anti-oxidant treatments on the bone blood vessels of T1DM mice. T1D mice received intraperitoneal injection of an anti-oxidant, N-acetyl cysteine (NAC) or tempol (TPO), once every other day from week 4 through 8. (A) Flow cytometry analysis of the general ROS levels in the bone ECs from 8-week-old tibia by detecting intracellular DCF fluorescence. The levels of blood glucose (B), insulin (C) and body weight (D). (E) Tibia length at week 8. (F) Confocal images of CD31 (green) and Emcn (red) immunostained tibia sections show type H vessels in the tibia metaphysis at week 8. Scale bar: 50 μm on the above and 20 μm on the below. Histomorphometric quantitation of the area fraction of vessel columns (G) and the number of vascular buds per growth plate length (H). Flow cytometry quantification of type H ECs (I) and total ECs (J) in 8-week-old tibia. (K) qPCR analysis of the representative cytokines mediating the coupling of angiogenesis and osteogenesis in 8-week-old tibia. (B)-(D), n = 15-16 per group; other panels, n = 7-8 per group. *p < 0.05, **p < 0.01 and ***p < 0.001. One-way or two-way ANOVA followed by Tukey posttest analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33664862), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Endothelial JNK-deficient mice display abnormal native collateral arteries.(A,B) Representative confocal images (n = 7 mice) of whole mount adductor muscle vasculature reveals SMA-covered gracilis collateral arteries in P6 control mice, but not JNK-deficient mice (A). Confocal imaging of Dil perfused P6 adductor muscle vasculature (n = 5 mice) demonstrates distinct gracilis collaterals interconnecting the PCFA to the SA in control mice. Vessels emerging from the PCFA and the SA in E3KO mice do not fully interconnect, but branch into smaller vessels. At P0, gracilis collaterals were not SMA-covered, but were perfused with Dil in control mice (B). The analogous vessels in E3KO mice did not interconnect, but branched extensively into smaller vessels (B) (n = 5 mice). (C) Representative stereomicroscope images of Dil-perfused abdominal muscle arterial vasculature of control and JNK-deficient P0 mice. Arteriole-to-arteriole arcades (indicated by red arrows) were quantitated (mean ± SEM; n = 3 mice). (D) Representative confocal images (n = 3~4 mice) of whole mount adductor muscles of control and JNK-deficient E16.5 embryos showing GFP-labeled vascular endothelial cells. (E) Representative confocal images (n = 3~5 mice) of control and JNK-deficient E16.5 embryo adductor muscle vasculature immunostained for Endomucin (Emcn, red) and isolectinB4 (iB4, green). Prominent vessels emerging from the PCFA and SA are indicated with white arrowheads. Source data are included as Figure 5—source data 1.DOI://dx.doi.org/10.7554/eLife.18414.02710.7554/eLife.18414.028Figure 5—source data 1.Source data for Figure 5.This file contains raw source data used to make the graphs presented in Figure 5.DOI://dx.doi.org/10.7554/eLife.18414.028Source data for Figure 5.This file contains raw source data used to make the graphs presented in Figure 5.DOI://dx.doi.org/10.7554/eLife.18414.028Intimate association of gracilis collaterals and peripheral nerves in adductor muscles.Confocal

$<b>Biological Strategies Validation. </b> The effects of insulin therapy on the bone blood vessels of T1DM mice. Drug therapy with high dose (hi, 0.5 U/mouse) or low dose (lo, 0.05 U/mouse) of insulin (ins) or the combination of insulin and a relatively low dose of GSK2795039 (GSK, a NOX2 inhibitor, 2 mM) was conducted in two time courses: started immediately after the confirmation of DM and once per day in week 4 to 8 (4-8w); started at week 6 and performed once per day in week 6 to 8 (6-8w). The levels of blood glucose (A) and body weight (B) were examined at week 4, 6 and 8, with HbA1c (C) tested at week 8. (D) Confocal images of immunostained tibia sections show CD31+ (green) and Emcn+ (red) type H vessels in the metaphysis. Scale bar: 50 μm on the above and 20 μm on the below. Histomorphometric quantitation as the area fraction of vessel columns (E) and the number of vascular buds per growth plate length (F). (G) Flow cytometry quantification of type H ECs and total ECs in 8-week-old tibia. For drug treatments, T1DM model was induced by STZ injection at 80 mg/kg (namely DM-G2). (A)-(C), n = 15-16 per group; (E)-(G), n = 6-8 per group. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. DM+veh group; #p < 0.05, ##p < 0.01 and ###p < 0.001 vs. DM+ins hi 6-8w group; &p < 0.05, &&p < 0.01 and &&&p < 0.001 vs. DM+ins lo 4-8w group. One-way or two-way ANOVA followed by Tukey posttest analysis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33664862), licensed under a CC-BY license. Not internally tested by R&D Systems.$

VE-cadherin expression is retained in the vasculature of affected organs in CYP19A1(Tg)Cre; Cdh5fl/fl embryos.(A) Immunofluorescence staining of E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl brains for VE-cadherin (green) and Endomucin (red) at sites of hemorrhage. Images in gray scale are VE-cadherin alone. Red arrowheads point to extravascular autofluorescent erythrocytes. Yellow arrowheads point to VE-cadherin+ vessels. Scale bars = 50 μm. (B) Immunofluorescence staining of E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl hearts for VE-cadherin (green) and Endomucin (red). Images in gray scale are VE-cadherin alone. Red arrowheads point to VE-cadherin+ developing coronary vessels. Yellow arrowheads point to VE-cadherin+ endocardium. Scale bars = 50 μm. (C) Immunofluorescence staining of E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl hearts for VE-cadherin (green), Endomucin (red), and LYVE1 (gray). Images in gray scale represent VE-cadherin pixels colocalized with either LYVE1 (liver) or Endomucin (lungs and thorax). Red arrowheads point to LYVE1+VE-cadherin+ liver sinusoidal vessels. Yellow arrowheads point to Endomucin+VE-cadherin+ lung and thoracic blood vessels. Scale bars = 200 μm. (D) Quantification of fold change in VE-cadherin mean fluorescence intensity in the brain, heart, liver, lungs, and thorax.Figure 1—figure supplement 3—source data 1.Excel file containing quantification for VE-cadherin expression in various organs in Figure 1—figure supplement 3.Excel file containing quantification for VE-cadherin expression in various organs in Figure 1—figure supplement 3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35486098), licensed under a CC-BY license. Not internally tested by R&D Systems.

VE-cadherin is required in trophoblasts to remodel spiral arteries (SAs)&to displace SA endothelium.(A) Immunofluorescence staining of E12.5 Control&CYP19A1(Tg)Cre; Cdh5fl/fl placentas for Endomucin (green)&alpha-smooth muscle actin ( alpha SMA) (red). White arrowheads indicate alpha SMA+ cells. Scale bars = 25 μm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35486098), licensed under a CC-BY license. Not internally tested by R&D Systems.

Loss of trophoblast VE-cadherin causes defects in brain, liver, and heart development.(A) Sagittal hematoxylin and eosin (H&E) sections from E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl embryos. Boxes show magnified regions of the brain, heart, and liver. Note the thinness of the myocardium in CYP19A1(Tg)Cre; Cdh5fl/fl embryos. Dotted red line outlines the liver contour. Green arrowhead points to tissue degradation in the brains of CYP19A1(Tg)Cre; Cdh5fl/fl embryos. (B) Quantification of the liver area as a percent of total embryo area. Control n = 4, CYP19A1(Tg)Cre; Cdh5fl/fl n = 3. (C, D) Immunofluorescence staining of E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl embryonic hearts for Endomucin (green) and alpha-smooth muscle actin ( alpha SMA) (red). Insets demonstrate myocardial thinning and quantified in (D). Control n = 11, CYP19A1(Tg)Cre; Cdh5fl/fl n = 5. Scale bars = 200 μm. Statistical analysis was performed using two-tailed, unpaired Welch’s t-test. Data are shown as means ± SD.Figure 1—figure supplement 2—source data 1.Excel file containing quantification for liver area and myocardial thickness in Figure 1—figure supplement 2.Excel file containing quantification for liver area and myocardial thickness in Figure 1—figure supplement 2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35486098), licensed under a CC-BY license. Not internally tested by R&D Systems.

VE-cadherin is required in trophoblasts to remodel spiral arteries (SAs)&to displace SA endothelium. (G, H) Maximum intensity projections of whole-mount immunofluorescence of the decidua (G)&labyrinth (H) from 200 μm thick placenta sections stained for Endomucin (green), TER119 (red),&CK8 (magenta). Double-headed arrows indicate differences in lumen size. White arrowheads indicate Endomucin+ SA ECs. Red arrowheads indicate maternal red blood cells within the trophoblast-lined vessels. Yellow arrowheads indicate fetal red blood cells within fetal capillaries. Dotted white line demarcates the decidua from the junctional zone. Scale bars = 50 μm. Statistical analysis was performed using two-tailed, unpaired Welch’s t-test. Data are shown as means ± SD.Figure 2—source data 1.Excel file containing quantification for smooth muscle cells per spiral artery, spiral artery diameter,&trophoblast-endothelial cell displacement in Figure 2.Excel file containing quantification for smooth muscle cells per spiral artery, spiral artery diameter,&trophoblast-endothelial cell displacement in Figure 2. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35486098), licensed under a CC-BY license. Not internally tested by R&D Systems.

Deletion of VE-cadherin in trophoblasts disrupts cell migration resulting in placental&fetal growth restriction. (I–L) Hematoxylin&eosin (H&E) staining&immunofluorescence staining for Endomucin (green), CK8 (red),&TER119 (gray) of E12.5 Control (I, J)&CYP19A1(Tg)Cre; Cdh5fl/fl (K, L) serial placenta sections. Dotted lines demarcate the different placental regions. Red arrowheads indicate trophoblasts. Green arrowheads indicate spiral arteries (SA). White arrowheads indicate maternal red blood cells (RBCs). Note fewer non-nucleated, maternal TER119+ cells in the labyrinth region of CYP19A1(Tg)Cre; Cdh5fl/fl placentas. Boxes on the left correlate with magnified images on the right,&boxes in H&E&immunofluorescence images are of the same region. Scale bars = 500 μm. Dec (decidua), JZ (junctional zone), Lab (labyrinth). Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35486098), licensed under a CC-BY license. Not internally tested by R&D Systems.

VE-cadherin is required in trophoblasts to remodel spiral arteries (SAs)&to displace SA endothelium.(D) Immunofluorescence staining of E12.5 Control&CYP19A1(Tg)Cre; Cdh5fl/fl placentas for Endomucin (green)&CK8 (red). White arrowheads indicate Endomucin+ SA endothelial cells (ECs). Positive signal in small, rounded cells in the lumen is the result of erythrocyte autofluorescence. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35486098), licensed under a CC-BY license. Not internally tested by R&D Systems.

Disrupting trophoblast migration results in decidual extracellular matrix defects.(A) Mmp15 mRNA gene expression in control and CYP19A1(Tg)Cre; Cdh5fl/fl placentas from RNA-sequencing (RNA-seq). (B) Immunofluorescence staining for Endomucin (green), CK8 (red), and MMP15 (gray) in E12.5 Control and CYP19A1(Tg)Cre; Cdh5fl/fl placentas. White arrowheads point to MMP15+CK8+ trophoblasts which are more abundant in control placentas relative compared to CYP19A1(Tg)Cre; Cdh5fl/fl placentas, particularly around spiral arteries (SA), but there is no difference in MMP15 intensity in trophoblasts. Dotted line demarcates the border of the junctional zone and decidua. Positive yellow signal in small, rounded cells in the SA lumen is the result of erythrocyte autofluorescence. Scale bars = 100 μm. (C) Immunofluorescence staining for laminin (red) and CK8 (green) and quantification of fold change in laminin mean fluorescence intensity in the stroma. In control placentas, laminin is expressed in trophoblasts surrounding SAs but absent in stromal cells compared to CYP19A1(Tg)Cre; Cdh5fl/fl placentas. Scale bars = 50 μm. (D) Immunofluorescence staining for CK8 (green), vinculin (red), and TER119 (gray) and quantification of fold change in vinculin mean fluorescence intensity in trophoblasts. Scale bars = 25 μm. Statistical analysis was performed using two-tailed, unpaired Welch’s t-test. Data are shown as means ± SD.Figure 4—figure supplement 1—source data 1.Excel file containing quantification for Mmp15 gene expression, laminin expression in the decidua, and vinculin expression in trophoblasts in Figure 4—figure supplement 1.Excel file containing quantification for Mmp15 gene expression, laminin expression in the decidua, and vinculin expression in trophoblasts in Figure 4—figure supplement 1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35486098), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF4666U-100UG
Alexa Fluor 405	AF4666V-100UG
Alexa Fluor 488	AF4666G-100UG
Alexa Fluor 532	AF4666X-100UG
Alexa Fluor 594	AF4666T-100UG
Alexa Fluor 647	AF4666R-100UG
Alexa Fluor 700	AF4666N-100UG
Alexa Fluor 750	AF4666S-100UG
Alexa Fluor Plus 405	AF4666AFP405-100UG
Alexa Fluor Plus 488	AF4666AFP488-100UG
Alexa Fluor Plus 555	AF4666AFP555-100UG
Alexa Fluor Plus 594	AF4666AFP594-100UG
Alexa Fluor Plus 647	AF4666AFP647-100UG
Alexa Fluor Plus 680	AF4666AFP680-100UG
Allophycocyanin	AF4666A
Biotin	AF4666B
CoraFluor 1	AF4666CL1
DyLight 350	AF4666D
DyLight 405	AF4666E
DyLight 488	AF4666K
DyLight 550	AF4666L
DyLight 594	AF4666M
DyLight 650	AF4666W
DyLight 680	AF4666Y
DyLight 755	AF4666Z
FITC	AF4666F
HRP	AF4666H
Janelia Fluor 525	AF4666JF525
Janelia Fluor 549	AF4666I
Janelia Fluor 646	AF4666J
Janelia Fluor 669	AF4666JF669
PE	AF4666P
PerCP	AF4666C
mFluor Violet 450 SE	AF4666MFV450
mFluor Violet 500 SE	AF4666MFV500
mFluor Violet 610 SE	AF4666MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4666-SP

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NBP1-92150

Endomucin/Mucin -14 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-92150

Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-92150-25ul

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AF7206

Endomucin/Mucin -14 Antibody

100 μg

AF7206

HAF019). Specific bands were detected for Endomucin at approximately 80 to 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7206) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). Specific bands were detected for Endomucin at approximately 80 to 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7206) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Endomucin at approximately 80 to 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF7206U-100UG
Alexa Fluor 405	AF7206V-100UG
Alexa Fluor 488	AF7206G-100UG
Alexa Fluor 532	AF7206X-100UG
Alexa Fluor 594	AF7206T-100UG
Alexa Fluor 647	AF7206R-100UG
Alexa Fluor 700	AF7206N-100UG
Alexa Fluor 750	AF7206S-100UG
Alexa Fluor Plus 405	AF7206AFP405-100UG
Alexa Fluor Plus 488	AF7206AFP488-100UG
Alexa Fluor Plus 555	AF7206AFP555-100UG
Alexa Fluor Plus 594	AF7206AFP594-100UG
Alexa Fluor Plus 647	AF7206AFP647-100UG
Alexa Fluor Plus 680	AF7206AFP680-100UG
Allophycocyanin	AF7206A
Biotin	AF7206B
CoraFluor 1	AF7206CL1
DyLight 350	AF7206D
DyLight 405	AF7206E
DyLight 488	AF7206K
DyLight 550	AF7206L
DyLight 594	AF7206M
DyLight 650	AF7206W
DyLight 680	AF7206Y
DyLight 755	AF7206Z
FITC	AF7206F
HRP	AF7206H
Janelia Fluor 525	AF7206JF525
Janelia Fluor 549	AF7206I
Janelia Fluor 646	AF7206J
Janelia Fluor 669	AF7206JF669
PE	AF7206P
PerCP	AF7206C
mFluor Violet 450 SE	AF7206MFV450
mFluor Violet 500 SE	AF7206MFV500
mFluor Violet 610 SE	AF7206MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7206-SP

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NBP3-11257

Endomucin/Mucin -14 Antibody (V.7C7.1)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Unconjugated

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NBP3-03542

Endomucin/Mucin -14 Antibody - BSA Free

100 ul

NBP3-03542

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-03542-20ul

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NBP2-07067

Endomucin/Mucin -14 Overexpression Lysate

0.1 mg

NBP2-07067

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-87360

Endomucin/Mucin -14 Antibody - BSA Free

100 ul

NBP2-87360

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-98900

Recombinant Human Endomucin/Mucin -14 His Protein

20 μg

NBP1-98900

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-48356

Endomucin/Mucin -14 Antibody (DMC473) - Chimeric

100 μg

NBP3-48356

Flow cytometry analysis with Anti-Endomucin/Mucin -14 (DMC473) on HEK293 cells transfected with human Endomucin/Mucin -14 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-92150PEP

Endomucin/Mucin -14 Recombinant Protein Antigen

0.1 ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF4666AFP594

Endomucin/Mucin -14 Antibody [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 594

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AF7206AFP594

Endomucin/Mucin -14 Antibody [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 594

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 51705

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