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![Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-ITEM-4-Flow-Cytometry-NBP2-00409-img0003.jpg "Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Analysis using the Biotin conjugate of NBP2-00409. Staining of TWEAK R in murine 603B cell lines using anti-TWEAK R antibody. Image from verified customer review.")
![Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) [NBP2-00409] - Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a K Isotype Control Purified (open histogram) or 0.5 ug Anti-Human/Mouse CD266 (TWEAK Receptor) Purified (filled histogram) followed byAnti-Mouse IgG FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-ITEM-4-Flow-Cytometry-NBP2-00409-img0001.jpg)
![Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099] - Separation of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (low endotoxin, concentration in sample 1 ug/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-ITEM-4-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09099-img0002.jpg "Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099] - Separation of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (low endotoxin, concentration in sample 1 ug/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")
![Flow Cytometry: TWEAK R/TNFRSF12 Antibody (ITEM-4) - Low Endotoxin, Azide and BSA Free [NBP3-09099] - Surface staining pattern of HUVEC cells stained using anti-human CD266 (ITEM-4) purified antibody (low endotoxin, concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-ITEM-4-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09099-img0001.jpg)
![Western Blot: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621] - Analysis of extracts of various cell lines, using TWEAK R/TNFRSF12 Rabbit mAb (NBP3-16621) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TWEAK-R-TNFRSF12-Antibody-1G4T3-Western-Blot-NBP3-16621-img0002.jpg "Western Blot: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621] - Analysis of extracts of various cell lines, using TWEAK R/TNFRSF12 Rabbit mAb (NBP3-16621) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621] - Immunofluorescence analysis of NIH-3T3 cells using TWEAK R/TNFRSF12 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16621_rabbit-tweak-r-tnfrsf12-mab-1g4t3-822025209820.jpg)
![Immunohistochemistry: TWEAK R/TNFRSF12 Antibody (7H8) [NBP3-26280] - Image of TWEAK R/TNFRSF12 Antibody (7H8) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26280_rabbit-tweak-r-tnfrsf12-mab-7h8-2722024850468.jpg "Immunohistochemistry: TWEAK R/TNFRSF12 Antibody (7H8) [NBP3-26280] - Image of TWEAK R/TNFRSF12 Antibody (7H8) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: TWEAK R/TNFRSF12 Antibody (7H8) [NBP3-26280] - Positive Western Blot detected in: U251 whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate. All lanes: TWEAK R/TNFRSF12 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14, 11 kD Observed band size: 14 kDa](http://images.novusbio.com/fullsize/nbp3-26280_rabbit-tweak-r-tnfrsf12-mab-7h8-28220241140914.jpg)