HAF016). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse liver tissue, mouse kidney tissue, rat liver tissue, rat kidney tissue, human liver tissue, and human kidney tissue. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat PCK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6787) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6787-SP | |||
HAF016). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue, human kidney tissue, mouse kidney tissue, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PCK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7316) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7316-SP | |||
HAF018). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PCK1 Monoclonal Antibody (Catalog # MAB7316) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PCK1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7316-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80927-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80928-25ul | |||
![Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Western-Blot-H00005105-M01-img0008.jpg "Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of monoclonal antibody to PCK1 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Immunocytochemistry-Immunofluorescence-H00005105-M01-img0005.jpg)
![Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - PCK1 monoclonal antibody (M01), clone 3E4 Analysis of PCK1 expression in HepG2.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Western-Blot-H00005105-M01-img0007.jpg)
![Immunoprecipitation: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 transfected lysate using anti-PCK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Immunoprecipitation-H00005105-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunocytochemistry-Immunofluorescence-NBP2-75609-img0003.jpg "Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609] - Analysis of paraffin-embedded human kidney tissue using anti-PCK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunohistochemistry-Paraffin-NBP2-75609-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunocytochemistry-Immunofluorescence-NBP2-75609-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (JU84-39) [NBP2-75609] - Staining PCK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunocytochemistry-Immunofluorescence-NBP2-75609-img0002.jpg)
![Immunohistochemistry-Paraffin: PCK1 Antibody (JU84-39) [NBP2-75609] - Analysis of paraffin-embedded human liver tissue using anti-PCK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PCK1-Antibody-JU84-39-Immunohistochemistry-Paraffin-NBP2-75609-img0004.jpg)
![Western Blot: PCK1 Antibody [NBP1-80927] - Analysis in human kidney tissue.](http://images.novusbio.com/fullsize/PCK1-Antibody-Western-Blot-NBP1-80927-img0006.jpg "Western Blot: PCK1 Antibody [NBP1-80927] - Analysis in human kidney tissue.")
![Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80927] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in renal tubules.](http://images.novusbio.com/fullsize/PCK1-Antibody-Immunohistochemistry-Paraffin-NBP1-80927-img0007.jpg)
![Western Blot: PCK1 Antibody [NBP1-80927] - Analysis in control (vector only transfected HEK293T lysate) and PCK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PCK1-Antibody-Western-Blot-NBP1-80927-img0008.jpg)
![Western Blot: PCK1 Antibody [NBP1-80928] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/PCK1-Antibody-Western-Blot-NBP1-80928-img0005.jpg "Western Blot: PCK1 Antibody [NBP1-80928] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: PCK1 Antibody [NBP1-80928] - Staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/PCK1-Antibody-Immunohistochemistry-Paraffin-NBP1-80928-img0002.jpg)
![Western Blot: PCK1 Antibody [NBP1-54720] - 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.](http://images.novusbio.com/fullsize/PCK1-Antibody-Western-Blot-NBP1-54720-img0009.jpg "Western Blot: PCK1 Antibody [NBP1-54720] - 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.")
![Western Blot: PCK1 Antibody [NBP1-54720] - Antibody Positive control: Lane 1 : 80 ug pig serum Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1 : 500 Submitted by: Martina Ondrovics, University of Veterinary Medicine Vienna.](http://images.novusbio.com/fullsize/PCK1-Antibody-Western-Blot-NBP1-54720-img0008.jpg)
![ELISA: Human PCK1 ELISA Kit (Colorimetric) [NBP2-81208] - Samples were spiked with high concentrations of Human PCK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81208_human-pck1-elisa-kit-colorimetric-13220241613023.jpg "ELISA: Human PCK1 ELISA Kit (Colorimetric) [NBP2-81208] - Samples were spiked with high concentrations of Human PCK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human PCK1 ELISA Kit (Colorimetric) [NBP2-81208] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81208_human-pck1-elisa-kit-colorimetric-432024856735.jpg)
![ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81241] - Samples were spiked with high concentrations of Rat PCK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81241_rat-pck1-elisa-kit-colorimetric-132202416133631.jpg "ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81241] - Samples were spiked with high concentrations of Rat PCK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81241] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81241_rat-pck1-elisa-kit-colorimetric-432024855077.jpg)
![ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81210] - Samples were spiked with high concentrations of Rat PCK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81210_rat-pck1-elisa-kit-colorimetric-132202416204039.jpg "ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81210] - Samples were spiked with high concentrations of Rat PCK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat PCK1 ELISA Kit (Colorimetric) [NBP2-81210] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81210_rat-pck1-elisa-kit-colorimetric-432024983589.jpg)
