NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Notch‑4 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human Notch‑4 Intracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3847) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3847-SP | |||
NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Notch-4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Notch-4 Intracellular Domain Monoclonal Antibody (Catalog # MAB3847) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3847-SP | |||
1506-D4) is immobilized at 2 ug/mL, Recombinant Human Notch-4 Fc Chimera (Catalog # 9909-NT) binds with an ED50 of 0.5-2.5 µg/mL." alt="When Recombinant Human DLL4 (Catalog # 1506-D4) is immobilized at 2 ug/mL, Recombinant Human Notch-4 Fc Chimera (Catalog # 9909-NT) binds with an ED50 of 0.5-2.5 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27755-50ul | |||
![Immunohistochemistry: Notch-4 Antibody [NBP3-10954] - Immunohistochemical analysis of human breast tissue at an antibody concentration of 5.0ug/ml](http://images.novusbio.com/fullsize/Notch-4-Antibody-Immunohistochemistry-NBP3-10954-img0001.jpg "Immunohistochemistry: Notch-4 Antibody [NBP3-10954] - Immunohistochemical analysis of human breast tissue at an antibody concentration of 5.0ug/ml")
![Western Blot: Notch-4 Antibody (SR3217) [NBP3-27755] - Western blot analysis of Mouse lung lysate.](http://images.novusbio.com/fullsize/nbp3-27755_rabbit-notch-4-mab-sr3217-75202410133454.jpg "Western Blot: Notch-4 Antibody (SR3217) [NBP3-27755] - Western blot analysis of Mouse lung lysate.")
![Western Blot: Notch4 Overexpression Lysate (Adult Normal) [NBP2-04274] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Notch4.](http://images.novusbio.com/fullsize/Notch4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04274-img0001.jpg "Western Blot: Notch4 Overexpression Lysate (Adult Normal) [NBP2-04274] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Notch4.")
![Western Blot: Notch-4 Antibody (S08-8H5) [NBP3-19822] - Western blot detection of Notch-4 in Hela, U87-MG cell lysates using NBP3-19822 (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.](http://images.novusbio.com/fullsize/Notch-4-Antibody-S08-8H5-Western-Blot-NBP3-19822-img0001.jpg "Western Blot: Notch-4 Antibody (S08-8H5) [NBP3-19822] - Western blot detection of Notch-4 in Hela, U87-MG cell lysates using NBP3-19822 (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.")
