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![Western Blot: c-Myb [p Thr486] Antibody [NBP2-21659] - Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and treated with okadaic acid to include phosphorylation of c-Myb. Load: 35 ug per lane. The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4C. Secondary antibody: Dye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: PBS-Tween overnight at 4C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb: wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.](http://images.novusbio.com/fullsize/c-Myb-[p-Thr486]-Antibody-Western-Blot-NBP2-21659-img0001.jpg "Western Blot: c-Myb [p Thr486] Antibody [NBP2-21659] - Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and treated with okadaic acid to include phosphorylation of c-Myb. Load: 35 ug per lane. The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4C. Secondary antibody: Dye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: PBS-Tween overnight at 4C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb: wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.")
![Western Blot: c-Myb Antibody [NBP2-15923] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/c-Myb-Antibody-Western-Blot-NBP2-15923-img0001.jpg "Western Blot: c-Myb Antibody [NBP2-15923] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: MYB Antibody [NBP3-10483] - Western blot analysis of MYB in Human 293T Whole Cell. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/MYB-Antibody-Western-Blot-NBP3-10483-img0001.jpg "Western Blot: MYB Antibody [NBP3-10483] - Western blot analysis of MYB in Human 293T Whole Cell. Antibody dilution at 1ug/ml")
![Western Blot: MYB Antibody [NBP3-10890] - Western blot analysis of MYB in OVCAR-3 Whole Cell lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/MYB-Antibody-Western-Blot-NBP3-10890-img0001.jpg "Western Blot: MYB Antibody [NBP3-10890] - Western blot analysis of MYB in OVCAR-3 Whole Cell lysates. Antibody dilution at 1.0ug/ml")
![Western Blot: c-Myb Overexpression Lysate (Adult Normal) [NBL1-13410] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myb.](http://images.novusbio.com/fullsize/c-Myb-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13410-img0002.jpg "Western Blot: c-Myb Overexpression Lysate (Adult Normal) [NBL1-13410] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for c-Myb.")
![Immunocytochemistry/Immunofluorescence: MYB [p Ser11] Antibody (3F4) [NBP3-15214] - Immunofluorescence staining of Hela cells with MYB antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/MYB-p-Ser11-Antibody-3F4-Immunocytochemistry-Immunofluorescence-NBP3-15214-img0001.jpg "Immunocytochemistry/Immunofluorescence: MYB [p Ser11] Antibody (3F4) [NBP3-15214] - Immunofluorescence staining of Hela cells with MYB antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).")
![Western Blot: c-Myb Antibody (JE40-07) [NBP3-32200] - Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (NBP3-32200) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: 3 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32200) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32200_rabbit-c-myb-mab-je40-07-278202415242635.jpg "Western Blot: c-Myb Antibody (JE40-07) [NBP3-32200] - Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (NBP3-32200) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: 3 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32200) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")