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![Western Blot: SARS-CoV-2 ORF8 Antibody [NBP3-11934] - Western Blot Validation with SARS-CoV-2 (COVID-19) ORF8 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF8 recombinant protein. Antibodies: SARS-CoV-2 ORF8, NBP3-11934, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF8-Antibody-Western-Blot-NBP3-11934-img0003.jpg "Western Blot: SARS-CoV-2 ORF8 Antibody [NBP3-11934] - Western Blot Validation with SARS-CoV-2 (COVID-19) ORF8 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF8 recombinant protein. Antibodies: SARS-CoV-2 ORF8, NBP3-11934, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL")
![Immunohistochemistry-Paraffin: SARS-CoV-2 ORF8 Antibody [NBP3-11934] - Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF8 in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 ORF8 antibody (NBP3-11934, 0.1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF8 protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF8-Antibody-Immunohistochemistry-Paraffin-NBP3-11934-img0001.jpg)
![ELISA: SARS-CoV-2 ORF8 Antibody [NBP3-11934] - ELISA Validation with SARS-CoV-2 (COVID-19) ORF8 Protein Antibodies: SARS-CoV-2 ORF8 Antibody, NBP3-11934. A direct ELISA was performed using SARS-CoV-2 ORF8 recombinant protein as coating antigen and the anti-SARS-CoV-2 ORF8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF8-Antibody-ELISA-NBP3-11934-img0002.jpg)
![Western Blot: SARS-CoV-2 ORF8 Antibody [NBP3-07972] - Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 (2019-nCoV) ORF8 Protein antibody at dilution of 1:1000.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF8-Antibody-Western-Blot-NBP3-07972-img0002.jpg "Western Blot: SARS-CoV-2 ORF8 Antibody [NBP3-07972] - Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 (2019-nCoV) ORF8 Protein antibody at dilution of 1:1000.")
![Western Blot: SARS-CoV-2 ORF8 Antibody [NBP3-05720] - Western blot using ORF8 polyclonal antibody (1:500) and 1 ug ORF8 Protein. Secondary Antibody: Goat anti-Rabbit IgG + HRP (1:20000). Exposure time: 10s.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF8-Antibody-Western-Blot-NBP3-05720-img0001.jpg "Western Blot: SARS-CoV-2 ORF8 Antibody [NBP3-05720] - Western blot using ORF8 polyclonal antibody (1:500) and 1 ug ORF8 Protein. Secondary Antibody: Goat anti-Rabbit IgG + HRP (1:20000). Exposure time: 10s.")
![Western Blot: SARS-CoV-2 ORF8 Antibody [NBP3-11935] - Western Blot Validation with SARS-CoV-2 (COVID-19) ORF8 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF8 recombinant protein. Antibodies: SARS-CoV-2 ORF8, NBP3-11935, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF8-Antibody-Western-Blot-NBP3-11935-img0002.jpg "Western Blot: SARS-CoV-2 ORF8 Antibody [NBP3-11935] - Western Blot Validation with SARS-CoV-2 (COVID-19) ORF8 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF8 recombinant protein. Antibodies: SARS-CoV-2 ORF8, NBP3-11935, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL")
![ELISA: SARS-CoV-2 ORF8 Antibody [NBP3-11935] - ELISA Validation Antibodies: SARS-CoV-2 ORF8 Antibody, NBP3-11935. A direct ELISA was performed using SARS-CoV-2 ORF8 immunogen peptide as coating antigen and the anti-SARS-CoV-2 ORF8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF8-Antibody-ELISA-NBP3-11935-img0001.jpg)
![SDS-Page: Recombinant SARS-CoV-2 ORF8 His Protein [NBP3-07089] - SARS-CoV-2 ORF8 Recombinant Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 80%.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-ORF8-His-Protein-SDS-Page-NBP3-07089-img0001.jpg "SDS-Page: Recombinant SARS-CoV-2 ORF8 His Protein [NBP3-07089] - SARS-CoV-2 ORF8 Recombinant Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 80%.")