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![Western Blot: SARS-CoV-2 ORF7a Antibody (3C9) [NBP3-13521] - Non-infected (-) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (NBP3-13521) diluted at 1:1000.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF7a-Antibody-3C9-Western-Blot-NBP3-13521-img0004.jpg "Western Blot: SARS-CoV-2 ORF7a Antibody (3C9) [NBP3-13521] - Non-infected (-) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (NBP3-13521) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 ORF7a Antibody (3C9) [NBP3-13521] - SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a stained by SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (NBP3-13521) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF7a-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP3-13521-img0005.jpg)
![Western Blot: SARS-CoV-2 ORF7a Antibody (3C9) [NBP3-13521] - WB analysis of SARS-CoV-infected Vero E6 cells harvested at different time points postinfection using NBP3-13521 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF7a-Antibody-3C9-Western-Blot-NBP3-13521-img0002.jpg)
![Western Blot: SARS-CoV-2 ORF7a Antibody (3C9) [NBP3-13521] - SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] detects SARS-CoV ORF7a protein by western blot analysis. Non-transfected (-) and SARS-CoV ORF7a-transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9] (NBP3-13521) diluted at 1:1000.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF7a-Antibody-3C9-Western-Blot-NBP3-13521-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 ORF7a Antibody (3C9) [NBP3-13521] - ICC/IF analysis of Vero E6 cells expressing ORF7a using NBP3-13521 SARS-CoV / SARS-CoV-2 (COVID-19) ORF7a antibody [3C9].](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF7a-Antibody-3C9-Immunocytochemistry-Immunofluorescence-NBP3-13521-img0001.jpg)
![Immunohistochemistry-Paraffin: SARS-CoV-2 ORF7a Antibody [NBP3-11937] - Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF7a in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 ORF7a antibody (NBP3-11937, 1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF7a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF7a-Antibody-Immunohistochemistry-Paraffin-NBP3-11937-img0001.jpg "Immunohistochemistry-Paraffin: SARS-CoV-2 ORF7a Antibody [NBP3-11937] - Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF7a in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 ORF7a antibody (NBP3-11937, 1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF7a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.")
![ELISA: SARS-CoV-2 ORF7a Antibody [NBP3-11937] - ELISA Validation Antibodies: SARS-CoV-2 ORF7a Antibody, NBP3-11937. A direct ELISA was performed using SARS-CoV-2 ORF7a immunogen peptide as coating antigen and the anti-SARS-CoV-2 ORF7a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF7a-Antibody-ELISA-NBP3-11937-img0002.jpg)
![ELISA: SARS-CoV-2 ORF7a Antibody [NBP3-11936] - ELISA Validation Antibodies: SARS-CoV-2 ORF7a Antibody, NBP3-11936. A direct ELISA was performed using SARS-CoV-2 ORF7a immunogen peptide as coating antigen and the anti-SARS-CoV-2 ORF7a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF7a-Antibody-ELISA-NBP3-11936-img0001.jpg "ELISA: SARS-CoV-2 ORF7a Antibody [NBP3-11936] - ELISA Validation Antibodies: SARS-CoV-2 ORF7a Antibody, NBP3-11936. A direct ELISA was performed using SARS-CoV-2 ORF7a immunogen peptide as coating antigen and the anti-SARS-CoV-2 ORF7a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL")