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![Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202413221512.jpg "Immunoprecipitation: Lamin A + C Antibody (4H7) [NBP3-26167] - Immunoprecipitating Lamin A + C in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26167 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26167 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-262202414471576.jpg)
![Immunohistochemistry: Lamin A + C Antibody (4H7) [NBP3-26167] - Image of Lamin A + C Antibody (4H7) diluted at 1:115 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-2722024820395.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (4H7) [NBP3-26167] - Staining of Hela cells with Lamin A + C Antibody (4H7) at 1:38, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202412493637.jpg)
![Flow Cytometry: Lamin A + C Antibody (4H7) [NBP3-26167] - Overlay histogram showing Hela cells stained with Lamin A + C Antibody (4H7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26167_rabbit-lamin-a-c-mab-4h7-282202414312758.jpg)
![Western Blot: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Western blot detection of Lamin A + C in 3T3, Hela cell lysates using NBP3-19963 (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 74, 63kDa.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-S06-7H5-Western-Blot-NBP3-19963-img0003.jpg "Western Blot: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Western blot detection of Lamin A + C in 3T3, Hela cell lysates using NBP3-19963 (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 74, 63kDa.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Immunohistochemistry of Lamin A + C in paraffin-embedded Human breast cancer tissue using NBP3-19963 at dilution 1/5](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-S06-7H5-Immunohistochemistry-Paraffin-NBP3-19963-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Immunofluorescence of Lamin A + C (green) in Hela cells using NBP3-19963 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19963_rabbit-lamin-a-c-mab-s06-7h5-113202515145450.jpg)
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![SDS-Page: Recombinant Human Lamin A Protein [H00004000-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004000-P01-1.jpg "SDS-Page: Recombinant Human Lamin A Protein [H00004000-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Lamin A + C Antibody (S07-1I5) [NBP3-19988] - Western blot detection of Lamin A + C in U251, Lncap, A549 using NBP3-19988 (1:1000 diluted)](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-S07-1I5-Western-Blot-NBP3-19988-img0002.jpg "Western Blot: Lamin A + C Antibody (S07-1I5) [NBP3-19988] - Western blot detection of Lamin A + C in U251, Lncap, A549 using NBP3-19988 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (S07-1I5) [NBP3-19988] - Immunohistochemistry of Lamin A + C in paraffin-embedded Human breast cancer tissue using NBP3-19988 at dilution 1/50](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-S07-1I5-Immunohistochemistry-Paraffin-NBP3-19988-img0001.jpg)
![Western Blot: Lamin A Overexpression Lysate (Adult Normal) [NBL1-12564] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lamin A.](http://images.novusbio.com/fullsize/Lamin-A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12564-img0002.jpg "Western Blot: Lamin A Overexpression Lysate (Adult Normal) [NBL1-12564] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lamin A.")
![Western Blot: Lamin A Overexpression Lysate (Adult Normal) [NBL1-12565] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lamin A.](http://images.novusbio.com/fullsize/Lamin-A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12565-img0001.jpg "Western Blot: Lamin A Overexpression Lysate (Adult Normal) [NBL1-12565] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lamin A.")
![ELISA: Human Lamin A + C - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39743] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39743_human-lamin-a-c-ready-to-use-elisa-kit-colorimetric-2110202415295615.png "ELISA: Human Lamin A + C - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39743] - Standard Curve Reference")
![ELISA: Human Lamin A + C ELISA Kit (Colorimetric) [NBP3-39742] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39742_human-lamin-a-c-elisa-kit-colorimetric-2110202415334928.png "ELISA: Human Lamin A + C ELISA Kit (Colorimetric) [NBP3-39742] - Standard Curve Reference")