Products Related to Gene-ID: 3965

HAF018). A specific band was detected for Galectin-9 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Galectin-9 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20456) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-9 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Galectin-9 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB2045) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB20451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20452) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB20451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20452) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Galectin-9 protein was serially diluted 2-fold and captured by Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20452) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20452) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20452) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Galectin-9 protein was serially diluted 2-fold and captured by Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20451) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Galectin-9 Monoclonal Antibody (Catalog # MAB20452) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

FC004). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> FC004). View our protocol for Staining Intracellular Molecules." alt="HEK293 cell line transfected with Galectin-9 (filled histogram) vs irrelevant HEK293 transfectant cells (open histogram) were stained with Rabbit Anti-Human Galectin‑9 Monoclonal Antibody (Catalog # MAB9064). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules." class="big_thumb" />