HAF005). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." alt="Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 18 kDa (as indicated) using 50 µg/mL of Rat Anti-Mouse Galectin‑1 Monoclonal Antibody (Catalog # MAB12451) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB12451-SP | |||
HAF005). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." alt="Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 18 kDa (as indicated) using 50 µg/mL of Rat Anti-Mouse Galectin‑1 Monoclonal Antibody (Catalog # MAB1245) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1245-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79855 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03266-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34878-50ug | |||
| Carrier-Free | NBP2-34878-1mg | |||
| Carrier-Free | NBP2-34878-100ug | |||
| Carrier-Free | NBP2-34878-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76490-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-21721-0.025mg | |||
![Western Blot: Galectin-1 Antibody (JM13-37) [NBP2-75499] - Western blot analysis of Galectin-1 on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-JM13-37-Western-Blot-NBP2-75499-img0006.jpg "Western Blot: Galectin-1 Antibody (JM13-37) [NBP2-75499] - Western blot analysis of Galectin-1 on human skeletal muscle tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (JM13-37) [NBP2-75499] - Staining Galectin 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-JM13-37-Immunocytochemistry-Immunofluorescence-NBP2-75499-img0002.jpg)
![Immunohistochemistry-Paraffin: Galectin-1 Antibody (JM13-37) [NBP2-75499] - Analysis of paraffin-embedded rat stomach tissue using anti-Galectin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-JM13-37-Immunohistochemistry-Paraffin-NBP2-75499-img0004.jpg)
![Flow Cytometry: Galectin-1 Antibody (JM13-37) [NBP2-75499] - Analysis of NIH-3T3 cells with Galectin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-JM13-37-Flow-Cytometry-NBP2-75499-img0001.jpg)
![Immunohistochemistry-Paraffin: Galectin-1 Antibody (JM13-37) [NBP2-75499] - Analysis of paraffin-embedded human placenta tissue using anti-Galectin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-JM13-37-Immunohistochemistry-Paraffin-NBP2-75499-img0003.jpg)
![Western blot analysis on different lysates with NBP2-75499 at 1/2,000 dilution. Lane 1: HL-60 cell lysate Lane 2: LNCaP cell lysate (negative) Lane 3: A549 cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 15 kDa Observed band size: 14 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-75499 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75499_-western-blot-639204586675734394.jpg)
![Western blot analysis on different lysates with NBP2-75499 at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: L6 cell lysate (20 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Predicted band size: 15 kDa Observed band size: 14 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-75499 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-75499_-western-blot-639204597491730137.jpg)
HAF005). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />
HAF005). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />
![Western Blot: Galectin-1 Antibody (GAL1/2499R) [NBP2-79721] - Western Blot Analysis of HeLa cell lysate using Galectin-1 antibody (GAL1/2499R).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-2499R-Western-Blot-NBP2-79721-img0004.jpg "Western Blot: Galectin-1 Antibody (GAL1/2499R) [NBP2-79721] - Western Blot Analysis of HeLa cell lysate using Galectin-1 antibody (GAL1/2499R).")
![Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/2499R) [NBP2-79721] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Galectin-1 Antibody (GAL1/2499R).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-2499R-Immunohistochemistry-Paraffin-NBP2-79721-img0001.jpg)
![Western Blot: Galectin-1 Antibody (GAL1/2499R) [NBP2-79721] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/2499R).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-2499R-Western-Blot-NBP2-79721-img0003.jpg)
![Protein Array: Galectin-1 Antibody (GAL1/2499R) [NBP2-79721] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Antibody (GAL1/2499R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-2499R-Protein-Array-NBP2-79721-img0002.jpg)
![Western Blot: Galectin-1 Antibody [NBP1-59002] - Titration: 0.2-1 ug/ml, Positive Control: Human Muscle.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP1-59002-img0008.jpg "Western Blot: Galectin-1 Antibody [NBP1-59002] - Titration: 0.2-1 ug/ml, Positive Control: Human Muscle.")
![Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP1-59002] - Galectin 1 Antibody [NBP1-59002] - Human placenta tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-59002-img0004.jpg)
![Western Blot: Galectin-1 Antibody [NBP1-59002] - Galectin 1 Antibody [NBP1-59002] - Mouse macrophages.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP1-59002-img0005.jpg)
![Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP1-59002] - Galectin 1 Antibody [NBP1-59002] - Human Placenta tissue 5ug/ml.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-59002-img0003.jpg)
![N/A Galectin-1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Galectin1_DY115205_ELISA_2342.jpg "N/A Galectin-1 [Biotin]")
![Western Blot: Galectin-1 Antibody [NBP3-03266] - Analysis of extracts from normal (control) and LGALS1 knockout (KO) 293T cells, using Galectin-1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Knockout-Validated-NBP3-03266-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Galectin-1 Antibody [NBP3-03266] - Analysis of extracts from normal (control) and LGALS1 knockout (KO) 293T cells, using Galectin-1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-1 Antibody - Azide and BSA Free [Galectin-1] - Western blot analysis of lysates from SGC-7901 cells using Galectin-1 Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03266_rabbit-polyclonal-galectin-1-antibody-5220251639690.jpg)
![SDS-Page: Human Galectin-1 Protein [NBP2-34878]](http://images.novusbio.com/fullsize/Human-Galectin-1-Protein-SDS-Page-NBP2-34878-img0001.jpg "SDS-Page: Human Galectin-1 Protein [NBP2-34878]")
![Western Blot: Galectin-1 Antibody [NBP2-76490] - Analysis using Anti-LGALS1 antibody NBP2-76490 (A) shows similar pattern to independent antibody NBP1-89791(B).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-76490-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Galectin-1 Antibody [NBP2-76490] - Analysis using Anti-LGALS1 antibody NBP2-76490 (A) shows similar pattern to independent antibody NBP1-89791(B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Galectin-1 Antibody [NBP2-76490] - Analysis in human cell lines PC-3 and Caco-2 using Anti-LGALS1 antibody. Corresponding LGALS1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-76490-img0001.jpg)
![Western Blot: Galectin-1 Antibody [NBP2-76499] - Analysis using Anti-LGALS1 antibody NBP2-76499130 (A) shows similar pattern to independent antibody NBP2-76490 (B).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-76499-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Galectin-1 Antibody [NBP2-76499] - Analysis using Anti-LGALS1 antibody NBP2-76499130 (A) shows similar pattern to independent antibody NBP2-76490 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Galectin-1 Antibody [NBP2-76499] - Analysis in human cell lines PC-3 and Caco-2 using Anti-LGALS1 antibody. Corresponding LGALS1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-76499-img0001.jpg)
![SDS-Page: Recombinant Mouse Galectin-1 Protein [NBP2-23182]](http://images.novusbio.com/fullsize/Recombinant-Mouse-Galectin-1-Protein-SDS-Page-NBP2-23182-img0002.jpg "SDS-Page: Recombinant Mouse Galectin-1 Protein [NBP2-23182]")
![Western Blot: Galectin-1 Antibody (RM0081-9J26) [NBP1-21721] - Analysis of mouse placental tissue lysates.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-RM0081-9J26-Western-Blot-NBP1-21721-img0001.jpg "Western Blot: Galectin-1 Antibody (RM0081-9J26) [NBP1-21721] - Analysis of mouse placental tissue lysates.")
