| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48139 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12014-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2776-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12013-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20720 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11896-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20717 | |||
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) - Azide and BSA Free [NBP3-11426] - Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 antibody (KRT7/903)followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-KRT7-903-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-11426-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) - Azide and BSA Free [NBP3-11426] - Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 antibody (KRT7/903)followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.")
![Western Blot: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric [NBP3-12014] - HeLa(A) (0.00001ug/ml), HepG2(B) (0.0003ug/ml) and A549(C) (0.00001ug/ml) cell lysate and human thyroid(D) (0.0003ug/ml) and human placenta(E) (0.00001ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 (NBP3-12014) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL-12-30-Chimeric-Western-Blot-NBP3-12014-img0001.jpg "Western Blot: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric [NBP3-12014] - HeLa(A) (0.00001ug/ml), HepG2(B) (0.0003ug/ml) and A549(C) (0.00001ug/ml) cell lysate and human thyroid(D) (0.0003ug/ml) and human placenta(E) (0.00001ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of OV-TL 12/30 (NBP3-12014) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric - Azide and BSA Free [NBP3-12014] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12014 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12014_rabbit-monoclonal-cytokeratin-7-antibody-ov-tl-12-30-chimeric-74202522113044.png)
![Immunocytochemistry /Immunofluorescence: Cytokeratin 7 Antibody (OV-TL 12/30) - Chimeric - Azide and BSA Free [NBP3-12014] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with NBP3-12014 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-12014, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12014_rabbit-monoclonal-cytokeratin-7-antibody-ov-tl-12-30-chimeric-74202522113016.png)
![Western Blot: Cytokeratin 7 Antibody (3-G3-D8-R) [NBP3-32253] - Western blot analysis of Cytokeratin 7 on different lysates with Mouse anti-Cytokeratin 7 antibody (NBP3-32253) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: SKOV-3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32253) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32253_mouse-cytokeratin-7-mab-3-g3-d8-r-27820241529557.jpg "Western Blot: Cytokeratin 7 Antibody (3-G3-D8-R) [NBP3-32253] - Western blot analysis of Cytokeratin 7 on different lysates with Mouse anti-Cytokeratin 7 antibody (NBP3-32253) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: SKOV-3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32253) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 7 Antibody (3-G3-D8-R) [NBP3-32253] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-Cytokeratin 7 antibody (NBP3-32253) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32253) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32253_mouse-cytokeratin-7-mab-3-g3-d8-r-29820241435264.jpg)
![Flow Cytometry: Cytokeratin 7 Antibody (3-G3-D8-R) [NBP3-32253] - Flow cytometric analysis of Hela cells labeling Cytokeratin 7. Cells were fixed and permeabilized, and then blocked with 2% negative goat serum for 15 minutes at room temperature.Then stained with the primary antibody (NBP3-32253, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32253_mouse-cytokeratin-7-mab-3-g3-d8-r-392024884656.jpg)
![Western Blot: Cytokeratin 7 Overexpression Lysate (Adult Normal) [NBL1-12396] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytokeratin 7.](http://images.novusbio.com/fullsize/Cytokeratin-7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12396-img0002.jpg "Western Blot: Cytokeratin 7 Overexpression Lysate (Adult Normal) [NBL1-12396] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cytokeratin 7.")
