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![Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85748] - Staining in human placenta and tonsil tissues using anti-ITGA6 antibody. Corresponding ITGA6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-Immunohistochemistry-Paraffin-NBP1-85748-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85748] - Staining in human placenta and tonsil tissues using anti-ITGA6 antibody. Corresponding ITGA6 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85748] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-Immunohistochemistry-Paraffin-NBP1-85748-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 6/CD49f Antibody [NBP1-85748] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-Immunohistochemistry-Paraffin-NBP1-85748-img0004.jpg)
![Western Blot: Integrin alpha 6/CD49f Antibody (2R5F1) [NBP3-16085] - Western blot analysis of extracts of various cell lines, using Integrin alpha 6/CD49f antibody (NBP3-16085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-2R5F1-Western-Blot-NBP3-16085-img0001.jpg "Western Blot: Integrin alpha 6/CD49f Antibody (2R5F1) [NBP3-16085] - Western blot analysis of extracts of various cell lines, using Integrin alpha 6/CD49f antibody (NBP3-16085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Integrin alpha 6/CD49f Antibody (2R5F1) [NBP3-16085] - Western blot analysis of extracts of various cell lines, using Integrin alpha 6/CD49f antibody (NBP3-16085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-2R5F1-Western-Blot-NBP3-16085-img0002.jpg)
![Western Blot: Integrin alpha 6/CD49f Antibody (4E4K6) [NBP3-15646] - Western blot analysis of extracts of A-431 cells, using Integrin alpha 6/CD49f (ITGA6/CD49f) antibody (NBP3-15646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Integrin-alpha-6-CD49f-Antibody-4E4K6-Western-Blot-NBP3-15646-img0001.jpg "Western Blot: Integrin alpha 6/CD49f Antibody (4E4K6) [NBP3-15646] - Western blot analysis of extracts of A-431 cells, using Integrin alpha 6/CD49f (ITGA6/CD49f) antibody (NBP3-15646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Integrin alpha 6/CD49f Antibody (4G10) [NBP3-26190] - Staining of MCF-7 cell with Integrin alpha 6/CD49f Antibody (4G10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 536-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26190_rabbit-integrin-alpha-6-cd49f-mab-4g10-28220241323627.jpg "Immunocytochemistry/Immunofluorescence: Integrin alpha 6/CD49f Antibody (4G10) [NBP3-26190] - Staining of MCF-7 cell with Integrin alpha 6/CD49f Antibody (4G10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 536-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Integrin alpha 6/CD49f Antibody (4G10) [NBP3-26190] - Overlay Peak curve showing Hela cells stained with Integrin alpha 6/CD49f Antibody (4G10) (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26190_rabbit-integrin-alpha-6-cd49f-mab-4g10-282202414183696.jpg)
![SDS-Page: Recombinant Human Integrin alpha 6/CD49f Protein [H00003655-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00003655-Q01-1.jpg "SDS-Page: Recombinant Human Integrin alpha 6/CD49f Protein [H00003655-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Integrin alpha 6 Overexpression Lysate (Adult Normal) [NBL1-12066] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 6.](http://images.novusbio.com/fullsize/Integrin-alpha-6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12066-img0002.jpg "Western Blot: Integrin alpha 6 Overexpression Lysate (Adult Normal) [NBL1-12066] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 6.")
![ELISA: Human Integrin alpha 6/CD49f ELISA Kit (Colorimetric) [NBP3-39955] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39955_human-integrin-alpha-6-cd49f-elisa-kit-colorimetric-2110202415355068.png "ELISA: Human Integrin alpha 6/CD49f ELISA Kit (Colorimetric) [NBP3-39955] - Standard Curve Reference")
![ELISA: Human Integrin alpha 6/CD49f - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39956] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39956_human-integrin-alpha-6-cd49f-ready-to-use-elisa-kit-2110202415355036.png "ELISA: Human Integrin alpha 6/CD49f - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39956] - Standard Curve Reference")