Products Related to Gene-ID: 3574

306-IR) was captured on Biacore Sensor Chip CM5 via Recombinant Protein A/G/L (Catalog # NBP2-34985), and binding to Animal-Free™ Recombinant Human IL 7 (Catalog # BT-007-AFL) was measured at a concentration range between 0.244 nM and 500 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=40.2 nM. (Biacore T200)." class="big_lightbox"> 306-IR) was captured on Biacore Sensor Chip CM5 via Recombinant Protein A/G/L (Catalog # NBP2-34985), and binding to Animal-Free™ Recombinant Human IL 7 (Catalog # BT-007-AFL) was measured at a concentration range between 0.244 nM and 500 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=40.2 nM. (Biacore T200)." class="big_thumb" />

PPK-007-GMP) stimulates proliferation of PHA-activated human peripheral blood lymphocytes. The ED₅₀ for this effect is 0.100-0.500 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of ProPak GMP IL‑7." class="big_lightbox"> PPK-007-GMP) stimulates proliferation of PHA-activated human peripheral blood lymphocytes. The ED₅₀ for this effect is 0.100-0.500 ng/mL. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of ProPak GMP IL‑7." class="big_thumb" />

PPK-007-GMP) and ProPak IL-15 (PPK-015-GMP) were added to Xeno-free Human T cell media in a bag (CCM038-GMP-1B). In parallel, liquid IL-7 (BT-007-GMP-025/LQ) and IL-15 (BT-015-GMP-025/LQ) or lyophilized IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) were added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). After cytokine addition, the concentration of IL-7 (SPCKB-CS-003676) and IL-15 (SPCKB-CS-003688) was determined by the Ella automated immunoassay system. Data is the average of 4 independent experiments with error bars ±SD. " class="big_lightbox"> PPK-007-GMP) and ProPak IL-15 (PPK-015-GMP) were added to Xeno-free Human T cell media in a bag (CCM038-GMP-1B). In parallel, liquid IL-7 (BT-007-GMP-025/LQ) and IL-15 (BT-015-GMP-025/LQ) or lyophilized IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) were added to Xeno-free Human T cell media in a bottle (CCM038-GMP-1L). After cytokine addition, the concentration of IL-7 (SPCKB-CS-003676) and IL-15 (SPCKB-CS-003688) was determined by the Ella automated immunoassay system. Data is the average of 4 independent experiments with error bars ±SD. " class="big_thumb" />

PPK-007-GMP) and ProPak IL-15 (PPK-015-GMP) were compared liquid IL-7 (BT-007-GMP-025/LQ) and IL-15 (BT-015-GMP-025/LQ) or lyophilized IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP). Each were added to Xeno-free Human T cell media in a bag (CCM038-GMP-1B) with 5% huAB Serum. T cells from 3 donors were activated and expanded in G-Rex bioreactors for 9 days and assessed for fold expansion. Data is the average of 4 independent experiments with 3 donors per experiment, error bars ±SD. " class="big_lightbox"> PPK-007-GMP) and ProPak IL-15 (PPK-015-GMP) were compared liquid IL-7 (BT-007-GMP-025/LQ) and IL-15 (BT-015-GMP-025/LQ) or lyophilized IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP). Each were added to Xeno-free Human T cell media in a bag (CCM038-GMP-1B) with 5% huAB Serum. T cells from 3 donors were activated and expanded in G-Rex bioreactors for 9 days and assessed for fold expansion. Data is the average of 4 independent experiments with 3 donors per experiment, error bars ±SD. " class="big_thumb" />

207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-7 Polyclonal Antibody (Catalog # AB-207-NA). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_lightbox"> 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-7 Polyclonal Antibody (Catalog # AB-207-NA). The ND₅₀ is typically 0.25-1.25 µg/mL." alt="Recombinant Human IL-7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-7 Polyclonal Antibody (Catalog # AB-207-NA). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_thumb" />

207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-7 Monoclonal Antibody (Catalog # MAB207R). The ND₅₀ is typically 0.4-0.8 µg/mL." class="big_lightbox"> 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-7 Monoclonal Antibody (Catalog # MAB207R). The ND₅₀ is typically 0.4-0.8 µg/mL." alt="Recombinant Human IL-7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-7 Monoclonal Antibody (Catalog # MAB207R). The ND₅₀ is typically 0.4-0.8 µg/mL." class="big_thumb" />

10531-IR) with a ED₅₀ of 0.200-2.00 μg/mL." class="big_lightbox"> 10531-IR) with a ED₅₀ of 0.200-2.00 μg/mL." alt="Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human IL‑7 Protein (Catalog # BTN007) binds to Recombinant Human IL-7R alpha/CD127 Fc Chimera (10531-IR) with a ED₅₀ of 0.200-2.00 μg/mL." class="big_thumb" />

407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse IL-7 Monoclonal Antibody (Catalog # MAB4071). The ND₅₀ is typically 0.5-3 µg/mL." class="big_lightbox"> 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse IL-7 Monoclonal Antibody (Catalog # MAB4071). The ND₅₀ is typically 0.5-3 µg/mL." alt="Recombinant Mouse IL-7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse IL-7 Monoclonal Antibody (Catalog # MAB4071). The ND₅₀ is typically 0.5-3 µg/mL." class="big_thumb" />

407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-7 Monoclonal Antibody (Catalog # MAB4072). The ND₅₀ is typically 75-450 ng/mL." class="big_lightbox"> 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-7 Monoclonal Antibody (Catalog # MAB4072). The ND₅₀ is typically 75-450 ng/mL." alt="Recombinant Mouse IL-7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-7 Monoclonal Antibody (Catalog # MAB4072). The ND₅₀ is typically 75-450 ng/mL." class="big_thumb" />