HAF017). A specific band was detected for Annexin A7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Annexin A7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3926) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3926-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57620-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70181 | |||
![Western Blot: Annexin A7 Antibody [NBP1-31442] - A. 30 ug 293T whole cell extractB. 30 ug A431 whole cell extractC. 30 ug HeLa whole cell extractD. 30 ug HepG2 whole cell extract10 % SDS-PAGE Annexin VII antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-Western-Blot-NBP1-31442-img0011.jpg "Western Blot: Annexin A7 Antibody [NBP1-31442] - A. 30 ug 293T whole cell extractB. 30 ug A431 whole cell extractC. 30 ug HeLa whole cell extractD. 30 ug HepG2 whole cell extract10 % SDS-PAGE Annexin VII antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Annexin A7 Antibody [NBP1-31442] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: ANXA7 protein stained by Annexin VII antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31442-img0006.jpg)
![Immunohistochemistry-Paraffin: Annexin A7 Antibody [NBP1-31442] - Paraffin-embedded human gastric carcinoma dilution: 1:500.](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-Immunohistochemistry-Paraffin-NBP1-31442-img0009.jpg)
![Immunohistochemistry: Annexin A7 Antibody (JE65-60) [NBP3-32038] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Annexin A7 antibody (NBP3-32038) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32038) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32038_rabbit-annexin-a7-mab-je65-60-298202414304149.jpg "Immunohistochemistry: Annexin A7 Antibody (JE65-60) [NBP3-32038] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Annexin A7 antibody (NBP3-32038) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32038) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32038 at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32038 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32038_-western-blot-639204597793735697.jpg)
![Western blot analysis of NIH/3T3 cell lysates with NBP3-32038 at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32038 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32038_-western-blot-639204598087586933.jpg)
![Western Blot: Annexin A7 Antibody (S07-1D2) [NBP3-19944] - Western blot detection of Annexin A7 in K562 (Lane 1), Rat Brain (Lane 2) cell lysates using NBP3-19944 (1:1000 diluted). Predicted band size: 53kDa. Observed band size: 53kDa.](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-S07-1D2-Western-Blot-NBP3-19944-img0002.jpg "Western Blot: Annexin A7 Antibody (S07-1D2) [NBP3-19944] - Western blot detection of Annexin A7 in K562 (Lane 1), Rat Brain (Lane 2) cell lysates using NBP3-19944 (1:1000 diluted). Predicted band size: 53kDa. Observed band size: 53kDa.")
![Immunohistochemistry-Paraffin: Annexin A7 Antibody (S07-1D2) [NBP3-19944] - Immunohistochemistry of Annexin A7 in paraffin-embedded Human tonsil using NBP3-19944 at dilution 1/50](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-S07-1D2-Immunohistochemistry-Paraffin-NBP3-19944-img0001.jpg)
![Western Blot: Annexin A7 Antibody [NBP2-57620] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-Western-Blot-NBP2-57620-img0002.jpg "Western Blot: Annexin A7 Antibody [NBP2-57620] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: Annexin A7 Antibody [NBP2-57620] - Staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57620-img0001.jpg)
![Western blot analysis of SH-SY5Y cell lysates with NBP3-32039 at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32039 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32039_-western-blot-639204597794017210.jpg "Western blot analysis of SH-SY5Y cell lysates with NBP3-32039 at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32039 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western blot analysis of rat brain tissue lysates with NBP3-32039 at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32039 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32039_-western-blot-639204598087650402.jpg)
![Western Blot: Annexin A7 Antibody (1C3) [NBP2-45494] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A7.](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-1C3-Western-Blot-NBP2-45494-img0001.jpg "Western Blot: Annexin A7 Antibody (1C3) [NBP2-45494] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A7.")
![Western Blot: Annexin A7 Antibody [NBP1-59099] - Human Placenta lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/Annexin-A7-Antibody-Western-Blot-NBP1-59099-img0002.jpg "Western Blot: Annexin A7 Antibody [NBP1-59099] - Human Placenta lysate, concentration 2.5 ug/ml.")
![Western Blot: Annexin A7 Overexpression Lysate (Adult Normal) [NBL1-07567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Annexin A7.](http://images.novusbio.com/fullsize/Annexin-A7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07567-img0002.jpg "Western Blot: Annexin A7 Overexpression Lysate (Adult Normal) [NBL1-07567] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Annexin A7.")
![Western Blot: Annexin A7 Overexpression Lysate (Adult Normal) [NBP2-10558] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Annexin A7.](http://images.novusbio.com/fullsize/Annexin-A7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10558-img0001.jpg "Western Blot: Annexin A7 Overexpression Lysate (Adult Normal) [NBP2-10558] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Annexin A7.")
