HAF005). A specific band was detected for Hck at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Hck Monoclonal Antibody (Catalog # MAB3915) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Hck at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3915-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57211-25ul | |||
![Western Blot: Hck Antibody (HL1673) - Azide and BSA Free [NBP3-25496] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (NBP3-25496) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25496_rabbit-hck-mab-hl1673-azide-and-bsa-free-212024939169.jpg "Western Blot: Hck Antibody (HL1673) - Azide and BSA Free [NBP3-25496] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (NBP3-25496) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Hck Antibody (HL1673) - Azide and BSA Free [NBP3-25496] - HCK antibody [HL1673] detects HCK protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. HCK stained by HCK antibody [HL1673] (NBP3-25496) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25496_rabbit-hck-mab-hl1673-azide-and-bsa-free-2120249413330.jpg)
![Western Blot: Hck Antibody (HL1673) - Azide and BSA Free [NBP3-25496] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (NBP3-25496) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25496_rabbit-hck-mab-hl1673-azide-and-bsa-free-2120249435520.jpg)
![Western Blot: Hck Antibody (HL1673) - Azide and BSA Free [NBP3-25496] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HCK antibody [HL1673] (NBP3-25496) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25496_rabbit-hck-mab-hl1673-azide-and-bsa-free-2120249362748.jpg)
![Immunohistochemistry-Paraffin: Hck Antibody (HL1673) - Azide and BSA Free [NBP3-25496] - HCK antibody [HL1673] detects HCK protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. HCK stained by HCK antibody [HL1673] (NBP3-25496) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25496_rabbit-hck-mab-hl1673-azide-and-bsa-free-2120249351387.jpg)
![Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02] - Analysis of protein-protein interactions between FLT1 and HCK. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-HCK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Hck-Antibody-2A6-Proximity-Ligation-Assay-H00003055-M02-img0002.jpg "Proximity Ligation Assay: Hck Antibody (2A6) [H00003055-M02] - Analysis of protein-protein interactions between FLT1 and HCK. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-HCK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: Hck Antibody (2A6) [H00003055-M02] - Detection limit for recombinant GST tagged HCK is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hck-Antibody-2A6-Sandwich-ELISA-H00003055-M02-img0003.jpg)
![Western Blot: Hck Antibody (3D12E10) [NBP1-47514] - Analysis using HCK mouse mAb against truncated HCK recombinant protein (1) and full-length HCK-GFP transfected CHO-K1 cell lysate (2).](http://images.novusbio.com/fullsize/Hck-Antibody-3D12E10-Western-Blot-NBP1-47514-img0005.jpg "Western Blot: Hck Antibody (3D12E10) [NBP1-47514] - Analysis using HCK mouse mAb against truncated HCK recombinant protein (1) and full-length HCK-GFP transfected CHO-K1 cell lysate (2).")
![Immunohistochemistry-Paraffin: Hck Antibody (3D12E10) [NBP1-47514] - Analysis of human colon cancer (left) and ancreas cancer (right), showing cytoplasmic localization using HCK mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hck-Antibody-3D12E10-Immunohistochemistry-Paraffin-NBP1-47514-img0004.jpg)
![Western Blot: Hck Antibody (S02-5C1) [NBP3-14957] - Western blot detection of Hck in Hela, A549, HL-60, U2OS cell lysates using Hck Rabbit mAb (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.](http://images.novusbio.com/fullsize/Hck-Antibody-S02-5C1-Western-Blot-NBP3-14957-img0001.jpg "Western Blot: Hck Antibody (S02-5C1) [NBP3-14957] - Western blot detection of Hck in Hela, A549, HL-60, U2OS cell lysates using Hck Rabbit mAb (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.")
![Immunocytochemistry/Immunofluorescence: Hck Antibody (S02-5C1) [NBP3-14957] - Immunocytochemistry of Hck (green) in hela using NBP3-14957 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14957_rabbit-hck-mab-s02-5c1-113202515145444.jpg)
![SDS-Page: Recombinant Human Hck Protein [H00003055-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Hck-Protein-SDS-Page-H00003055-P01-img0001.jpg "SDS-Page: Recombinant Human Hck Protein [H00003055-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Hck Antibody (1D9-1A9) [H00003055-M01] - Analysis of HCK expression in transfected 293T cell line by HCK monoclonal antibody (M01), clone 1D9-1A9.Lane 1: HCK transfected lysate (Predicted MW: 57.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Hck-Antibody-1D9-1A9-Western-Blot-H00003055-M01-img0002.jpg "Western Blot: Hck Antibody (1D9-1A9) [H00003055-M01] - Analysis of HCK expression in transfected 293T cell line by HCK monoclonal antibody (M01), clone 1D9-1A9.Lane 1: HCK transfected lysate (Predicted MW: 57.3 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: Hck Antibody (1D9-1A9) [H00003055-M01] - Analysis of HCK transfected lysate using anti-HCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HCK MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Hck-Antibody-1D9-1A9-Immunoprecipitation-H00003055-M01-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Hck Antibody [NBP2-57211] - Staining of human cell line SiHa shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize/Hck-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57211-img0001.jpg "Immunocytochemistry/Immunofluorescence: Hck Antibody [NBP2-57211] - Staining of human cell line SiHa shows localization to nucleoplasm & plasma membrane.")
![Western Blot: Hck Overexpression Lysate (Adult Normal) [NBP2-08146] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hck.](http://images.novusbio.com/fullsize/Hck-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08146-img0001.jpg "Western Blot: Hck Overexpression Lysate (Adult Normal) [NBP2-08146] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hck.")
