| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33444-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38849-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98608-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98608-100ul | |||
| BSA Free | NBP2-98608-50ul | |||
![Western Blot: PKN3 Antibody [NBP1-30102] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/PKN3-Antibody-Western-Blot-NBP1-30102-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PKN3 Antibody [NBP1-30102] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.1 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: PKN3 Antibody [NBP1-30102] - Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PKN3-Antibody-Western-Blot-NBP1-30102-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PKN3 Antibody [NBP1-30102] - PKN3 regulates growth in 2D and in 3D environment of Src-transformed MEFs through interaction with p130CaS. Immunoblot of SC cells and SC cells with PKN3 gene inactivated using CRISPR/CAS9 (SCpkn3-/-). Inactivation of PKN3 expression is visualized by antibody anti-PKN3. Antibody anti-actin was used as loading control. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1002/1878-0261.12401), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PKN3-Antibody-Knockout-Validated-NBP1-30102-img0005.jpg)
![Western Blot: PKN3 Antibody [NBP1-30102] - Influence of TP Agonist on the Activation of PRK1, PRK2 & PRK3. Panel C. In order to identify the species of PRK subject to U46619-induced T-loop phosphorylation, PC-3 cells initially transfected for 72 hr with 30 nM siRNAPRK1, siRNAPRK2, siRNAPRK3 or, as controls, with a scrambled siRNAControl. Thereafter, cells serum starved & stimulated with U46619 for 60 min or with vehicle & then immunoblotted (20 μg/lane) with anti-phospho-PRK1Thr774/PRK2Thr816/PRK3Thr718 & with anti-PRK1, anti-PRK2, anti-PRK3 or, as loading controls, HDJ2 antisera, as indicated. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.4664), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30102_rabbit-polyclonal-pkn3-antibody-310202415291986.jpg)
![Western Blot: PKN3 Antibody [NBP1-30102] - Effect of siRNA-mediated disruption of PRK1, PRK2 & PRK3 expression on TP agonist-induced proliferation & migration of PC-3 cellsPanels A-D. PC-3 cells were transfected for 48 hr with 30 nM siRNAPRK1, siRNAPRK2, siRNAPRK3 or, as controls, with a scrambled siRNA (siRNAControl) prior to stimulation with U46619 (10 nM) or vehicle (0.0001% EtOH) for the indicated time specific to the assay, where non-transfected cells served as a reference. Panel A: For analysis of proliferation, 72 hr post-siRNA transfection PC-3 cells were stimulated for 48 hr with U46619 (10 nM) or vehicle (0.0001% EtOH). Panel B: For analysis of colony formation, 48 hr post-siRNA transfection PC-3 cells were stimulated with U46619 (10 nM) or vehicle (0.0001% EtOH) in soft agar & assessed 10 day post-treatment for colony formation. Panel C: For analysis of migration, 72 hr post-siRNA transfection PC-3 cells were stimulated for 8 hr with U46619 (10 nM) or vehicle (0.0001% EtOH). In Panels A-C, the bar charts show mean relative levels of PC-3 cell proliferation, colony formation & migration (± SEM, n ≥ 3), where levels in the vehicle-treated cells are assigned a value of 100%. The asterisks indicate where U46619-stimulation resulted in significant increases in proliferation, colony formation or migration by PC-3 cells compared to vehicle-treated cells, where ** & *** indicates p 50 measurements – 3 per cell; ***P < 0.001, one‐way ANOVA on ranks followed by Dunn's post hoc test). (C) Src‐transformed p130Cas−/−MEFs co‐expressing p130Cas (SC) & mouse Flag tagged PKN3 WT or Flag‐PKN3 mPR are shown. Cells were grown on FN‐coated coverslips for 48 h, fixed, & stained for p130Cas by anti‐pTyr165 p130Cas antibody (pY165 p130Cas; 2nd 405), for actin by Phalloidin 488 & for Flag‐PKN3 by anti‐Flag antibody (2nd 633). Reflection (670 nm) indicates fibronectin degradation. All scale bars represent 20 μm. Cell were imaged by Leica TCS SP8 microscope system equipped with Leica 63×/1.45 oil objective. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30422386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30102_rabbit-polyclonal-pkn3-antibody-310202416154626.jpg)
![Western Blot: PKN3 Antibody [NBP1-30102] - PKN3 overexpression regulates growth of MEFs, & this effect requires PKN3–p130Cas interaction. (A) Immunoblotted lysates from MEFs p130Cas−/− or MEFs p130Cas−/− re‐expressing p130Cas (p130Cas+) treated by Doxycycline (Dox) to induce expression of mCherry‐PKN3 or mCherry alone. p130Cas presence detected by ani‐p130Cas antibody & mCherry epitope by anti‐mCherry antibody. (B) Dynamics of mCherry‐PKN3 expression after supplementation w/ Dox shown by immunoblot w/ anti‐mCherry antibody. (C–E) Effect of induced mCherry‐PKN3 expression on cell growth. Representative graphs showing growth of MEFs p130Cas−/− re‐expressing p130Cas (p130Cas+) (C) or MEFs p130Cas−/− (D) measured in real‐time using xCELLigence RTCA (real‐time cell analysis) system instrument. (E) Quantification of cell growth change induced by mCherry‐PKN3 expression (‘−’ indicates inducible mCherry expression used as negative control). Slope ratios reflecting cell growth calculated from log growth phase of cell growth (indicated by dotted lines; see C & D). (F) Immunoblotted lysates from MEFs p130Cas−/− re‐expressing p130Cas (p130Cas+) treated or not treated by Dox which induced expression of Flag‐fused PKN3 variants (WT, mPR, KD, empty vector). Stimulated overexpression of PKN3 detected by anti‐PKN3 antibody & its activity by antibody anti‐pT849 PKN3. (G) Quantification of cell growth change stimulated by Dox‐inducible expression of Flag‐fused PKN3 variants (WT, mPR, KD) in MEFs p130Cas−/− re‐expressing p130Cas (p130Cas+). All error bars indicate means ± SD calculated from 3 to 5 independent experiments (each in triplicates). Statistical significance always calculated between induced & noninduced cells & evaluated by one‐way repeated ANOVA followed by Turkey post hoc test (***P < 0.001). Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/30422386), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30102_rabbit-polyclonal-pkn3-antibody-31020241617355.jpg)
![Immunohistochemistry-Paraffin: PKN3 Antibody [NBP2-33444] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PKN3-Antibody-Immunohistochemistry-NBP2-33444-img0001.jpg "Immunohistochemistry-Paraffin: PKN3 Antibody [NBP2-33444] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.")
![Western Blot: PKN3 Antibody [H00029941-B01P] - Analysis of PKN3 expression in transfected 293T cell line by PKN3 polyclonal antibody. Lane 1: PKN3 transfected lysate(97.79 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PKN3-Antibody-Western-Blot-H00029941-B01P-img0002.jpg "Western Blot: PKN3 Antibody [H00029941-B01P] - Analysis of PKN3 expression in transfected 293T cell line by PKN3 polyclonal antibody. Lane 1: PKN3 transfected lysate(97.79 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: PKN3 Antibody [NBP2-38849] - Staining of human kidney shows moderate cytoplasmic positivity renal tubules.](http://images.novusbio.com/fullsize/PKN3-Antibody-Immunohistochemistry-Paraffin-NBP2-38849-img0003.jpg "Immunohistochemistry-Paraffin: PKN3 Antibody [NBP2-38849] - Staining of human kidney shows moderate cytoplasmic positivity renal tubules.")
![Western Blot: PKN3 Overexpression Lysate (Adult Normal) [NBL1-14464] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PKN3.](http://images.novusbio.com/fullsize/PKN3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14464-img0002.jpg "Western Blot: PKN3 Overexpression Lysate (Adult Normal) [NBL1-14464] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PKN3.")
![Western Blot: PKN3 Antibody [H00029941-D01P] - Analysis of PKN3 expression in transfected 293T cell line by PKN3 polyclonal antibody.Lane 1: PKN3 transfected lysate(99.40 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PKN3-Antibody-Western-Blot-H00029941-D01P-img0002.jpg "Western Blot: PKN3 Antibody [H00029941-D01P] - Analysis of PKN3 expression in transfected 293T cell line by PKN3 polyclonal antibody.Lane 1: PKN3 transfected lysate(99.40 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: PKN3 Antibody [NBP2-98608] - Immunochemical staining of human PKN3 in human breast with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKN3-Antibody-Immunohistochemistry-Paraffin-NBP2-98608-img0001.jpg "Immunohistochemistry-Paraffin: PKN3 Antibody [NBP2-98608] - Immunochemical staining of human PKN3 in human breast with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")