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![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-278202416331264.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Western blot analysis of Glutathione S-Transferase pi 1/GSTP1 on different lysates with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32411) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32411) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-29820241435285.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione S-Transferase pi 1/GSTP1 Antibody (11D5-R) [NBP3-32411] - Immunocytochemistry analysis of HT-29 cells labeling Glutathione S-Transferase pi 1/GSTP1 with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione S-Transferase pi 1/GSTP1 antibody (NBP3-32411) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32411_mouse-glutathione-s-transferase-pi-1-gstp1-mab-11d5-r-2920241192326.jpg)
![Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) [NBP3-26540] - Image of Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26540_rabbit-glutathione-s-transferase-pi-1-gstp1-mab-8f6-262202415355724.jpg "Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) [NBP3-26540] - Image of Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) [NBP3-26540] - Positive Western Blot detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: Glutathione S-Transferase pi 1/GSTP1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 24 kDa Observed band size: 24 kDa](http://images.novusbio.com/fullsize/nbp3-26540_rabbit-glutathione-s-transferase-pi-1-gstp1-mab-8f6-28220241152522.jpg)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (003) [NBP2-89323] - Lane A: Jurkat Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:23 kDa](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-003-Western-Blot-NBP2-89323-img0001.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (003) [NBP2-89323] - Lane A: Jurkat Whole Cell Lysate Lane B: K562 Whole Cell LysateLane C: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:23 kDa")
![<b>Genetic Strategies Validation. </b>NBP2-89323 used at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: GSTP1 knockout Hela Whole Cell Lysate. Lysates loaded at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa.](http://images.novusbio.com/fullsize/nbp2-89323_rabbit-monoclonal-esam-antibody-202-45202316595485.png)
![Western Blot: GSTP1 Overexpression Lysate (Adult Normal) [NBL1-11376] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSTP1.](http://images.novusbio.com/fullsize/GSTP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11376-img0002.jpg "Western Blot: GSTP1 Overexpression Lysate (Adult Normal) [NBL1-11376] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GSTP1.")
![Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Chemiluminescence) [NBP3-25938] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-25938_human-glutathione-s-transferase-pi-1-gstp1-elisa-kit-chem-161202410548.jpg "Human Glutathione S-Transferase pi 1/GSTP1 ELISA Kit (Chemiluminescence) [NBP3-25938] - Standard Curve Reference")