DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="PD-L1 was detected in immersion fixed paraffin-embedded sections of human Lung Tumor using Rabbit Anti-Human PD-L1, Monoclonal Antibody (Catalog #NBP2-23740) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24107 | |||
9814-PD) is coated at 1 µg/mL, Recombinant Rat PD-L1/B7-H1 Fc Chimera (Catalog # 9835-B7) binds with an ED50 of 0.09-0.54 µg/mL." alt="When Recombinant Rat PD-1 Fc Chimera (Catalog # 9814-PD) is coated at 1 µg/mL, Recombinant Rat PD-L1/B7-H1 Fc Chimera (Catalog # 9835-B7) binds with an ED50 of 0.09-0.54 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21060 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20876 | |||
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-5220251734540.jpg "Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517315539.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody (4V8L4) [PD-L1] - Western blot analysis of lysates from wild type (WT) and PD-L1 knockout (KO) A-549 cells using PD-L1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Wild type (WT) and PD-L1 knockout (KO) A549 cells were treated by IFNG (100 ng/mL) at 37C for 48 hours.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 30 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517315579.jpg)
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517311099.jpg)
![N/A PD-L1 [Biotin]](http://images.novusbio.com/fullsize/dy9326_monkey-pd-l1-b7-h1-duoset-elisa-15-plate-65202012351.jpg "N/A PD-L1 [Biotin]")
