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![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-5220251734540.jpg "Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517315539.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody (4V8L4) [PD-L1] - Western blot analysis of lysates from wild type (WT) and PD-L1 knockout (KO) A-549 cells using PD-L1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Wild type (WT) and PD-L1 knockout (KO) A549 cells were treated by IFNG (100 ng/mL) at 37C for 48 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517315579.jpg)
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517311099.jpg)
![N/A PD-L1 [Biotin]](http://images.novusbio.com/fullsize2/dy9326_monkey-pd-l1-b7-h1-duoset-elisa-15-plate-65202012351.jpg "N/A PD-L1 [Biotin]")
![Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32732_rabbit-pd-l1-mab-pd01-02-29820241492673.jpg "Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: PD-L1 Antibody (PD01-02) [NBP3-32732] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: U-87 MG cell lysate Lane 4: HepG2 cell lysate (low expression) Lane 5: HeLa cell lysate Lane 6: A549 cell lysate Lane 7: A549 treated with 100ng/mL IFN gamma for 48 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 45-50 kDa Exposure time: 25 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32732_rabbit-pd-l1-mab-pd01-02-3920248453543.jpg)
