Products Related to Gene-ID: 29126

F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) PD-L1/B7-H1 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561R) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

DY990). Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Goat Anti-Human PD-L1/B7-H1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF156) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

F0135). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135). View our protocol for Staining Membrane-associated Proteins." alt="MDA-MB-231 human breast adenocarcinoma cell line was stained with Human Anti-Human PD-L1/B7-H1 (Research Grade Durvalumab Biosimilar) Monoclonal Antibody (Catalog # MAB10355, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

1021-PD) with an ED₅₀ of 15.0‑150 ng/mL." class="big_lightbox"> 1021-PD) with an ED₅₀ of 15.0‑150 ng/mL." alt="Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Mouse PD-L1/B7-H1 His-tag Avi-tag Protein (Catalog # AVI9048) binds to Recombinant Mouse PD-1 Fc Chimera Protein (1021-PD) with an ED₅₀ of 15.0‑150 ng/mL." class="big_thumb" />

8578-PD) binds with an ED₅₀ of 15-75 ng/mL." class="big_lightbox"> 8578-PD) binds with an ED₅₀ of 15-75 ng/mL." alt="When Recombinant Cynomolgus Monkey PD-L1/B7-H1 (Catalog # 10145-B7) is immobilized at 1 µg/mL, Recombinant Cynomolgus Monkey PD-1 Fc Chimera (Catalog # 8578-PD) binds with an ED₅₀ of 15-75 ng/mL." class="big_thumb" />

1086-PD) is coated at 1 µg/mL, Biotinylated Recombinant Human PD-L1/B7-H1 Fc Chimera Avi-tag (Catalog # AVI156) binds with an ED₅₀ of 8-48 ng/mL." class="big_lightbox"> 1086-PD) is coated at 1 µg/mL, Biotinylated Recombinant Human PD-L1/B7-H1 Fc Chimera Avi-tag (Catalog # AVI156) binds with an ED₅₀ of 8-48 ng/mL." alt="When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 1 µg/mL, Biotinylated Recombinant Human PD-L1/B7-H1 Fc Chimera Avi-tag (Catalog # AVI156) binds with an ED₅₀ of 8-48 ng/mL." class="big_thumb" />

1086-PD) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 µg/mL." class="big_lightbox"> 1086-PD) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 µg/mL." alt="When Recombinant Porcine PD-L1/B7-H1 His-tag Protein (Catalog # 10361-B7) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 µg/mL." class="big_thumb" />

1021-PD) is immobilized at 2.5 μg/mL (100 μL/well), Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein (Catalog # AVI1019) binds with an ED₅₀ of 0.0600-0.600 μg/mL." class="big_lightbox"> 1021-PD) is immobilized at 2.5 μg/mL (100 μL/well), Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein (Catalog # AVI1019) binds with an ED₅₀ of 0.0600-0.600 μg/mL." alt="Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-1 Fc Chimera Protein (1021-PD) is immobilized at 2.5 μg/mL (100 μL/well), Biotinylated Recombinant Mouse PD-L1/B7-H1 Fc Chimera Avi-tag Protein (Catalog # AVI1019) binds with an ED₅₀ of 0.0600-0.600 μg/mL." class="big_thumb" />

10553-PD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED₅₀ of 0.30-4.50 µg/mL." class="big_lightbox"> 10553-PD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED₅₀ of 0.30-4.50 µg/mL." alt="When Recombinant Canine PD-1 Fc Chimera (10553-PD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Canine PD-L1/B7-H1 Fc Chimera (Catalog # 10746-B7) binds with an ED₅₀ of 0.30-4.50 µg/mL." class="big_thumb" />