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![Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.](http://images.novusbio.com/fullsize/PD-L1-Antibody-29E-2A3-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09098-img0002.jpg "Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.")
![Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/PD-L1-Antibody-29E-2A3-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09098-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunofluorescence of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml. Green: PD-L1 Antibody [F2G2] Blue: DAPI staining](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12826-img0002.jpg "Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunofluorescence of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml. Green: PD-L1 Antibody [F2G2] Blue: DAPI staining")
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12826-img0003.jpg)
![ELISA: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Titration ELISA of PD-L1.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-ELISA-NBP3-12826-img0001.jpg)
![Western Blot: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10ug/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1ug/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32731) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-27820241631671.jpg "Western Blot: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10ug/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1ug/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32731) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32731) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-29820241433368.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F6A9-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12825-img0001.jpg "Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.")
![Flow Cytometry: PD-L1 Antibody (F6A9) - VHH [NBP3-12825] - Flow cytometry analysis of PD-L1 transfected 293 cells using PD-L1 [F6A9] antibody at 10 ug/ml. Blue: untransfected cells, Yellow: PD-L1 transfected cells.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F6A9-VHH-Flow-Cytometry-NBP3-12825-img0002.jpg)
![SDS-Page: Recombinant Human PD-L1/B7-H1 Protein [NBP1-98984]](http://images.novusbio.com/fullsize/Recombinant-Human-B7-H1-PD-L1-CD274-Protein-SDS-Page-NBP1-98984-img0002.jpg "SDS-Page: Recombinant Human PD-L1/B7-H1 Protein [NBP1-98984]")
![Western Blot: CD274 Overexpression Lysate (Adult Normal) [NBP2-07377] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD274.](http://images.novusbio.com/fullsize/CD274-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07377-img0001.jpg "Western Blot: CD274 Overexpression Lysate (Adult Normal) [NBP2-07377] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD274.")
![Sandwich ELISA: CD274 Antibody Pair [H00029126-AP41] - Detection sensitivity ranging from 0.3 ng/ml to 100 ng/ml.](http://images.novusbio.com/fullsize/CD274-Antibody-Pair-Sandwich-ELISA-H00029126-AP41-img0001.jpg "Sandwich ELISA: CD274 Antibody Pair [H00029126-AP41] - Detection sensitivity ranging from 0.3 ng/ml to 100 ng/ml.")