Products Related to Gene-ID: 29126

HAF008). A specific band was detected for PD-L1/B7-H1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for PD-L1/B7-H1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PD-L1/B7-H1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

8986-PD, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 2.5 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (MAB1562) but not by (B) Rabbit IgG Isotype Control (MAB1050). Ligand binding was detected by staining cells with Anti-His tag APC-conjugated Monoclonal Antibody (IC1050A)." class="big_lightbox"> 8986-PD, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 2.5 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (MAB1562) but not by (B) Rabbit IgG Isotype Control (MAB1050). Ligand binding was detected by staining cells with Anti-His tag APC-conjugated Monoclonal Antibody (IC1050A)." class="big_thumb" />

AFR1086, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 25 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) but not by (B) Rabbit IgG Isotype Control (MAB1050). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> AFR1086, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 25 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) but not by (B) Rabbit IgG Isotype Control (MAB1050). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

F0111). View our protocol for Staining Membrane-associated Proteins. View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). View our protocol for Staining Membrane-associated Proteins. View our protocol for Staining Membrane-associated Proteins." alt="RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90783), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). View our protocol for Staining Membrane-associated Proteins." alt="RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open historgram) was stained with Rat Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB9078), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB006) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody ." class="big_lightbox"> MAB006) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody ." class="big_thumb" />

1086-PD) is immobilized at 2 µg/mL, Biotinylated Recombinant Human PD-L1 His-tag Avi-tag protein binds with an ED₅₀ of 2-12 µg/mL." class="big_lightbox"> 1086-PD) is immobilized at 2 µg/mL, Biotinylated Recombinant Human PD-L1 His-tag Avi-tag protein binds with an ED₅₀ of 2-12 µg/mL." alt="When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is immobilized at 2 µg/mL, Biotinylated Recombinant Human PD-L1 His-tag Avi-tag protein binds with an ED₅₀ of 2-12 µg/mL." class="big_thumb" />

F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_lightbox"> F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_thumb" />

8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM." class="big_lightbox"> 8986-PD) was measured at a concentration range between 3.2 nM and 13.2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=1.122 µM." class="big_thumb" />

F0135). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135). View our protocol for Staining Membrane-associated Proteins." alt="MDA-MB-231 human breast adenocarcinoma cell line was stained with Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

8986-PD, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348) but not by (B) Human IgG Isotype Control (1-001-A). Ligand binding was detected by staining cells with Anti-His tag APC-conjugated Monoclonal Antibody (IC050A)." class="big_lightbox"> 8986-PD, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348) but not by (B) Human IgG Isotype Control (1-001-A). Ligand binding was detected by staining cells with Anti-His tag APC-conjugated Monoclonal Antibody (IC050A)." class="big_thumb" />

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_lightbox"> DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="PD-L1/B7-H1 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse PD-L1/B7-H1 Recombinant Monoclonal Antibody (Catalog # MAB11630) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL004) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL004) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

1021-PD) with an ED₅₀ of 8-40 ng/mL." class="big_lightbox"> 1021-PD) with an ED₅₀ of 8-40 ng/mL." alt="When Recombinant Mouse PD-L1/B7-H1 is immobilize at 25 ng/mL, it binds Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) with an ED₅₀ of 8-40 ng/mL." class="big_thumb" />