HAF005). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse kidney tissue and mouse uterus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB2335) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2335-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89242-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90345-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26507-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47892-0.1mg | |||
HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![N/A Aminopeptidase N/CD13 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Aminopeptidase_N_DY3815_ELISA_1982.jpg "N/A Aminopeptidase N/CD13 [Biotin]")
![Western Blot: Aminopeptidase N/CD13 Antibody (122) [NBP2-89242] - Lane A: THP1 Whole Cell Lysate Lane B: U937 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:110 kDa Observed band size:140 kDa](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-122-Western-Blot-NBP2-89242-img0003.jpg "Western Blot: Aminopeptidase N/CD13 Antibody (122) [NBP2-89242] - Lane A: THP1 Whole Cell Lysate Lane B: U937 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:110 kDa Observed band size:140 kDa")
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (122) [NBP2-89242] - Staining of human ANPEP in human liver with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-122-Immunohistochemistry-Paraffin-NBP2-89242-img0002.jpg)
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (122) [NBP2-89242] - Staining of human ANPEP in human renal carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-122-Immunohistochemistry-Paraffin-NBP2-89242-img0001.jpg)
![Aminopeptidase N/CD13 Antibody (RM403) [NBP3-23461] - Immunohistochemical staining of formalin fixed and paraffin embedded human liver section](http://images.novusbio.com/fullsize/nbp3-23461_rabbit-aminopeptidase-n-cd13-mab-20620241444151.png "Aminopeptidase N/CD13 Antibody (RM403) [NBP3-23461] - Immunohistochemical staining of formalin fixed and paraffin embedded human liver section")
![Western Blot: Aminopeptidase N/CD13 Antibody (RM403) [NBP3-23461] - Western Blot of HepG2 cells lysate using Clone RM403) at a 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-23461_rabbit-aminopeptidase-n-cd13-mab-226202422321231.png)
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (101) [NBP2-90345] - Staining of mouse ANPEP in mouse intestines with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-101-Immunohistochemistry-Paraffin-NBP2-90345-img0002.jpg "Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (101) [NBP2-90345] - Staining of mouse ANPEP in mouse intestines with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (101) [NBP2-90345] - Staining of mouse ANPEP in mouse kidney with Rabbit Monoclonal Antibody (1:300).](http://images.novusbio.com/fullsize/Aminopeptidase-N-CD13-Antibody-101-Immunohistochemistry-Paraffin-NBP2-90345-img0001.jpg)
![Immunohistochemistry: Aminopeptidase N/CD13 Antibody (PD00-39) [NBP3-32123] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Aminopeptidase N/CD13 antibody (NBP3-32123) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32123_rabbit-aminopeptidase-n-cd13-mab-pd00-39-298202414161827.jpg "Immunohistochemistry: Aminopeptidase N/CD13 Antibody (PD00-39) [NBP3-32123] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Aminopeptidase N/CD13 antibody (NBP3-32123) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32123 at 1/2,000 dilution. Lane 1: PANC-1 cell lysate (5 µg/Lane) Lane 2: THP-1 cell lysate (30 µg/Lane) Lane 3: U-937 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (negative) (20 µg/Lane) Lane 5: HL-60 cell lysate (20 µg/Lane) Lane 6: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 110 kDa Observed band size: 150 kDa Exposure time: Lane 1: 9 seconds; Lane 2-5: 24 seconds; Lane 6: 1 minute 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32123 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32123_-western-blot-639204594550240581.jpg)
![Immunohistochemistry: Aminopeptidase N/CD13 Antibody (6E9) [NBP3-26507] - Image of Aminopeptidase N/CD13 Antibody (6E9) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26507_rabbit-aminopeptidase-n-cd13-mab-6e9-262202415475648.jpg "Immunohistochemistry: Aminopeptidase N/CD13 Antibody (6E9) [NBP3-26507] - Image of Aminopeptidase N/CD13 Antibody (6E9) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Aminopeptidase N/CD13 Antibody (6E9) [NBP3-26507] - Image of Aminopeptidase N/CD13 Antibody (6E9) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26507_rabbit-aminopeptidase-n-cd13-mab-6e9-27220248274915.jpg)
![Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric) [NBP2-77015] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-77015_mouse-aminopeptidase-n-cd13-elisa-kit-colorimetric-3120248504624.png "Mouse Aminopeptidase N/CD13 ELISA Kit (Colorimetric) [NBP2-77015] - Standard Curve Reference")
