MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">
MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR22 Monoclonal Antibody (Catalog # MAB4500, filled histogram) or isotype control antibody (Catalog #
MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')
2Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR22 Monoclonal Antibody (Catalog # MAB4500, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
![Western Blot: GPR22 Antibody [NBP3-15567] - Western blot analysis of extracts of various cell lines, using GPR22 antibody (NBP3-15567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/GPR22-Antibody-Western-Blot-NBP3-15567-img0003.jpg "Western Blot: GPR22 Antibody [NBP3-15567] - Western blot analysis of extracts of various cell lines, using GPR22 antibody (NBP3-15567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: GPR22 Antibody [NBP3-15567] - Immunofluorescence analysis of U-2 OS cells using GPR22 antibody (NBP3-15567) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GPR22-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15567-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GPR22 Antibody [NBP3-15567] - Immunofluorescence analysis of L929 cells using GPR22 antibody (NBP3-15567) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GPR22-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15567-img0001.jpg)
![Immunohistochemistry-Paraffin: GPR22 Antibody [NLS109] - Analysis of anti-GPR22 antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize/GPR22-Antibody-Immunohistochemistry-Paraffin-NLS109-img0001.jpg "Immunohistochemistry-Paraffin: GPR22 Antibody [NLS109] - Analysis of anti-GPR22 antibody with human brain, neurons and glia.")
