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HAF016). A specific band was detected for a-Galactosidase A/GLA at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">
HAF016). A specific band was detected for a-Galactosidase A/GLA at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human a-Galactosidase A/GLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6146) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Galactosidase A/GLA at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
NL010). LAMP1 was also detected using Mouse Anti-Human LAMP1 Mono-clonal Antibody (Catalog # MAB4800). Cells were stained using the Northern-Lights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Cells were counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">
NL010). LAMP1 was also detected using Mouse Anti-Human LAMP1 Mono-clonal Antibody (Catalog # MAB4800). Cells were stained using the Northern-Lights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Cells were counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">
HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />