MAB1817) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rat Anti-Mouse TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB18171) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
MAB1817) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rat Anti-Mouse TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB18171) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Mouse TIM-1/KIM-1/HAVCR protein was serially diluted 2-fold and captured by Rat Anti-Mouse TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog #
MAB1817) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Rat Anti-Mouse TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB18171) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
MAB1817) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB18171) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse TIM-1/KIM-1/HAVCR protein was serially diluted 2-fold and captured by Rat Anti-Mouse TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1817) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB18171) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
![ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-ELISA-NBP2-81119-img0001.jpg "ELISA: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (NBP2-81119; red line) and isotype control (NBP2-52638, anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIM-1-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using NBP2-81119. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP antibody was used.")
![Surface Plasmon Resonance: TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119] - Surface plasmon resonance (SPR) sensorgram TIM-1/KIM-1/HAVCR Antibody (3B3) [NBP2-81119]. 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein to immbolised the rabbit IgG chimeric version of 3B3 [NBP2-81119]. TIM-1 Fc-fusion protein was injected and allowed to associate with NBP2-81119 between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. TIM-1 Fc-fusion protein showed rapid binding to NBP2-81119 and following ligand removal remained strongly bound. This suggests that NBP2-81119 has a very high affinity for TIM-1 Fc-fusion protein.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-3B3-Surface-Plasmon-Resonance-NBP2-81119-img0002.jpg)
![SDS-Page: Recombinant Human TIM-1/KIM-1/HAVCR Protein [NBP2-52037] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Recombinant-Protein-SDS-Page-NBP2-52037-img0001.jpg "SDS-Page: Recombinant Human TIM-1/KIM-1/HAVCR Protein [NBP2-52037] - 15% SDS Page (3 ug)")
![Sandwich ELISA: TIM-1/KIM-1/HAVCR Antibody Pair [HRP] [NBP2-79564] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/TIM-1-KIM-1-HAVCR-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79564-img0001.jpg "Sandwich ELISA: TIM-1/KIM-1/HAVCR Antibody Pair [HRP] [NBP2-79564] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
