7955-IL) is immobilized at 0.500 μg/mL, 100 μL/well, Biotinylated Recombinant Human IL-17RA/IL-17R Fc Chimera Avi-tag Protein (Catalog # AVI177) binds with an ED50 of 5.00-30.0 ng/mL." alt="Measured by its binding ability in a functional ELISA. When Recombinant human IL-17A (7955-IL) is immobilized at 0.500 μg/mL, 100 μL/well, Biotinylated Recombinant Human IL-17RA/IL-17R Fc Chimera Avi-tag Protein (Catalog # AVI177) binds with an ED50 of 5.00-30.0 ng/mL." class="big_thumb" />
7955-IL) with an ED50 of 3.00-30.0 ng/mL." alt="Recombinant Human IL-17RA/IL-17R Avi-tag His-tag Protein (Catalog # AVI11234) binds to IL-17A (Human Cell-expressed) Protein (7955-IL) with an ED50 of 3.00-30.0 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21174-25ul | |||
| Azide and BSA Free | NBP3-44336 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21175-25ul | |||
| Azide and BSA Free | NBP3-44337 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22223-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79318-5Plates | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89700-50ul | |||
7955-IL)." class="big_thumb" />
7955-IL)." alt="Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050‑0.500 µg/mL in the presence of 10.0 ng/mL Recombinant Human IL‑17A Protein (
![Immunocytochemistry/ Immunofluorescence: IL-17RA/IL-17R Antibody (JE35-89) [NBP3-32467] - Immunocytochemistry analysis of HeLa cells labeling IL-17RA/IL-17R with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32467_rabbit-il-17ra-il-17r-mab-je35-89-2920241114827.jpg "Immunocytochemistry/ Immunofluorescence: IL-17RA/IL-17R Antibody (JE35-89) [NBP3-32467] - Immunocytochemistry analysis of HeLa cells labeling IL-17RA/IL-17R with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Western Blot: IL-17RA/IL-17R Antibody (SR1226) [NBP3-22223] - Western blot analysis using NBP3-22223 in Raji cell lysate.](http://images.novusbio.com/fullsize/nbp3-22223_rabbit-il-17ra-il-17r-mab-sr1226-57202317161868.jpg "Western Blot: IL-17RA/IL-17R Antibody (SR1226) [NBP3-22223] - Western blot analysis using NBP3-22223 in Raji cell lysate.")
![Sandwich ELISA: IL-17RA/IL-17R Antibody Pair [HRP] [NBP2-79318] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/IL-17RA-IL-17R-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79318-img0001.jpg "Sandwich ELISA: IL-17RA/IL-17R Antibody Pair [HRP] [NBP2-79318] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
