| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21174-25ul | |||
| Azide and BSA Free | NBP3-44336 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21175-25ul | |||
| Azide and BSA Free | NBP3-44337 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21174-100ul | |||
| BSA Free | NBP3-21174-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22223-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79318-5Plates | |||
![Western Blot: IL-17RA/IL-17R Antibody (JE35-89) [NBP3-32467] - Western blot analysis of IL-17RA/IL-17R on different lysates with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: Raji cell lysate Lane 6: HL-60 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 96 kDa Observed band size: 160 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32467) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32467_rabbit-il-17ra-il-17r-mab-je35-89-27820241631611.jpg "Western Blot: IL-17RA/IL-17R Antibody (JE35-89) [NBP3-32467] - Western blot analysis of IL-17RA/IL-17R on different lysates with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: Raji cell lysate Lane 6: HL-60 cell lysate Lysates/proteins at 25 ug/Lane. Predicted band size: 96 kDa Observed band size: 160 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32467) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: IL-17RA/IL-17R Antibody (JE35-89) [NBP3-32467] - Immunocytochemistry analysis of HeLa cells labeling IL-17RA/IL-17R with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-17RA/IL-17R antibody (NBP3-32467) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32467_rabbit-il-17ra-il-17r-mab-je35-89-2920241114827.jpg)
![Western Blot: IL-17RA/IL-17R Antibody (6Q2U9) [NBP3-16577] - Analysis of extracts of various cell lines, using IL-17RA/IL-17R Rabbit mAb (NBP3-16577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/IL-17RA-IL-17R-Antibody-6Q2U9-Western-Blot-NBP3-16577-img0001.jpg "Western Blot: IL-17RA/IL-17R Antibody (6Q2U9) [NBP3-16577] - Analysis of extracts of various cell lines, using IL-17RA/IL-17R Rabbit mAb (NBP3-16577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: IL-17RA/IL-17R Antibody (SR1226) [NBP3-22223] - Western blot analysis using NBP3-22223 in Raji cell lysate.](http://images.novusbio.com/fullsize/nbp3-22223_rabbit-il-17ra-il-17r-mab-sr1226-57202317161868.jpg "Western Blot: IL-17RA/IL-17R Antibody (SR1226) [NBP3-22223] - Western blot analysis using NBP3-22223 in Raji cell lysate.")
![Sandwich ELISA: IL-17RA/IL-17R Antibody Pair [HRP] [NBP2-79318] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/IL-17RA-IL-17R-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79318-img0001.jpg "Sandwich ELISA: IL-17RA/IL-17R Antibody Pair [HRP] [NBP2-79318] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")