Recombinant Human IL-17RA/IL-17R His-tag Protein, CF

Recombinant Human IL-17RA/IL-17R His-tag Protein (Catalog # 11234-IR) has a molecular weight (MW) of 50.4 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050‑1.00 µg/mL in the presence of 10.0 ng/mL Recombinant Human IL‑17A (7955-IL).

2 μg/lane of Recombinant Human IL-17RA/IL-17R His-tag Protein (Catalog # 11234-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-68 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-17RA/IL-17R His-tag Protein, CF Summary

• Additional Information: Analyzed by SEC-MALS
• Details of Functionality: Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.050‑1.00 µg/mL in the presence of 10.0 ng/mL recombinant human IL‑17 (Catalog # 7955-IL).
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-17RA/IL-17R protein
  Leu33-Trp320, with a C-terminal 6-His tag.
• Accession #: NP_055154.3
• N-terminal Sequence: Leu33
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-68 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17RA/IL-17R His-tag Protein, CF

• CD217 antigen
• CD217
• Cdw217
• CDw217interleukin 17 receptor
• hIL-17R
• IL-17 R
• IL-17 RA
• IL-17 receptor A
• IL17RA
• IL-17RA
• IL-17RAMGC10262
• IL17Rinterleukin-17 receptor A
• interleukin 17 receptor A

Background

Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL‑17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy‑proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL‑17 includes activation of NF‑ kappa B and JNK, and require TNF receptor‑associated factors 6 (TRAF6) in the signaling pathway.

1. Yao, Z. et al. (1997) Cytokine 9:794.
2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Bioinformatics

• Uniprot:
  • NP_055154.3()