Products Related to Gene-ID: 1956

236-EG) for 5 minutes. PVDF membrane was probed with 0.05 µg/mL of Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8058) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y1197) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 236-EG) for 5 minutes. PVDF membrane was probed with 0.05 µg/mL of Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8058) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y1197) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.05 µg/mL of Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8058) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y1197) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." alt="A431 human epithelial carcinoma cell line was stained with Rat Anti-Human EGFR Monoclonal Antibody (Catalog # MAB10951, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

4289-EG) with an ED₅₀ of < 600 ng/mL." class="big_lightbox"> 4289-EG) with an ED₅₀ of < 600 ng/mL." alt="Recombinant Cynomolgus Monkey EGFR His-tag (Catalog # 10405-ER) binds Recombinant Human Pro-EGF (aa21-1023) Protein (Catalog # 4289-EG) with an ED₅₀ of < 600 ng/mL." class="big_thumb" />

MAB9577) is immobilized at 0.25 µg/mL (100 µL/ well), Biotinylated Recombinant Human EGFR His-tag Avi-tag (AVI10493) that produces a 50% optimal binding response is found to be 1.5-10 ng/mL." class="big_lightbox"> MAB9577) is immobilized at 0.25 µg/mL (100 µL/ well), Biotinylated Recombinant Human EGFR His-tag Avi-tag (AVI10493) that produces a 50% optimal binding response is found to be 1.5-10 ng/mL." alt="When Human EGFR (Research Grade Cetuximab Biosimilar) Antibody (MAB9577) is immobilized at 0.25 µg/mL (100 µL/ well), Biotinylated Recombinant Human EGFR His-tag Avi-tag (AVI10493) that produces a 50% optimal binding response is found to be 1.5-10 ng/mL." class="big_thumb" />

AVI10493) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa." class="big_lightbox"> AVI10493) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa." class="big_thumb" />

MAB9577) with an ED₅₀ of 1.50-18.0 ng/mL." class="big_lightbox"> MAB9577) with an ED₅₀ of 1.50-18.0 ng/mL." alt="Biotinylated Recombinant Human EGFR His-tag Protein (Catalog # BT11302) binds Human EGFR (Research Grade Cetuximab Biosimilar) Antibody (MAB9577) with an ED₅₀ of 1.50-18.0 ng/mL." class="big_thumb" />

MAB9577) is immobilized at 0.25 µg/mL, 100 µL/well, the concentration of Recombinant Human EGFR Fc Chimera Avi-tag (Catalog # AVI344) that produces 50% of the optimal binding response is approximately 2-15 ng/mL." class="big_lightbox"> MAB9577) is immobilized at 0.25 µg/mL, 100 µL/well, the concentration of Recombinant Human EGFR Fc Chimera Avi-tag (Catalog # AVI344) that produces 50% of the optimal binding response is approximately 2-15 ng/mL." alt="When Human EGFR (Research Grade Cetuximab Biosimilar) Antibody (Catalog # MAB9577) is immobilized at 0.25 µg/mL, 100 µL/well, the concentration of Recombinant Human EGFR Fc Chimera Avi-tag (Catalog # AVI344) that produces 50% of the optimal binding response is approximately 2-15 ng/mL." class="big_thumb" />