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![Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 134.3 kDa, ~170 kDa for EGFR pY1197.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-[p-Tyr1197]-Antibody-Western-Blot-NBP1-77936-img0003.jpg "Western Blot: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Lane 1: unstimulated A431 cells. Lane 2: A431 cells stimulated with EGF (50 ng/ml for 15 min). Load: 30 ug per lane. Primary antibody: EGFR pY1197 antibody at 1:250 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 134.3 kDa, ~170 kDa for EGFR pY1197.")
![Immunohistochemistry-Paraffin: EGF R/ErbB1 [p Tyr1197] Antibody [NBP1-77936] - Tissue: placental trophoblasts. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: EGFR pY1197 antibody at 5ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: EGFR pY1197 is on the cell membrane. Staining: EGFR pY1197 as precipitated red signal with hematoxylin purple counterstain.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-[p-Tyr1197]-Antibody-Immunohistochemistry-Paraffin-NBP1-77936-img0002.jpg)
![Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13231] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13231) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Western-Blot-NBP3-13231-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13231] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13231) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1148] Antibody [NBP3-13231] - EGFR (phospho Tyr1148) antibody detects EGFR (phospho Tyr1148) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1148) protein stained by EGFR (phospho Tyr1148) antibody (NBP3-13231) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13231-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1148] Antibody [NBP3-13231] - EGFR (phospho Tyr1148) antibody detects EGFR protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1148) antibody (NBP3-13231) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Immunohistochemistry-Paraffin-NBP3-13231-img0003.jpg)
![Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13266] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Western-Blot-NBP3-13266-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13266] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1045] Antibody [NBP3-13266] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13266-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1045] Antibody [NBP3-13266] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1045) antibody (NBP3-13266) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunohistochemistry-Paraffin-NBP3-13266-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr998) protein stained by EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr998]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13267-img0002.jpg "Immunocytochemistry/Immunofluorescence: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr998) protein stained by EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: EGFR [p Tyr998] Antibody [NBP3-13267] - EGFR (phospho Tyr998) antibody detects EGFR (phospho Tyr998) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr998) antibody (NBP3-13267) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr998]-Antibody-Immunohistochemistry-Paraffin-NBP3-13267-img0003.jpg)
![Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13229] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Western-Blot-NBP3-13229-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1045] Antibody [NBP3-13229] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1045] Antibody [NBP3-13229] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1045) protein stained by EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13229-img0002.jpg)
![Immunohistochemistry-Paraffin: EGFR [p Tyr1045] Antibody [NBP3-13229] - EGFR (phospho Tyr1045) antibody detects EGFR (phospho Tyr1045) protein at cell membrane in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. EGFR (phospho Tyr1045) antibody (NBP3-13229) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1045]-Antibody-Immunohistochemistry-Paraffin-NBP3-13229-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52651_rabbit-monoclonal-egfr-antibody-528-chimeric-74202522141227.png "Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")