F0135). View our protocol for Staining Membrane-associated Proteins." alt="A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9577, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
HAF018). A specific band was detected for EGFR (aa 746-750 deletion) at approximately 170 kDa (as indicated). For additional reference total EGFR was detected using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF231). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HCC827 human non-small cell lung cancer cell line, HCT-116 human colorectal carcinoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EGFR (aa 746-750 deletion) Monoclonal Antibody (Catalog # MAB8336) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EGFR (aa 746-750 deletion) at approximately 170 kDa (as indicated). For additional reference total EGFR was detected using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # AF231). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8336-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84815-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34628-0.1mg | |||
AVI10494) binds with an ED50 of 25-150 ng/mL." alt="When Human EGFR Isoform vIII antibody (Novus Catalog # BNP-50599) is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human EGFR Isoform vIII Fc Chimera Avi-tag (AVI10494) binds with an ED50 of 25-150 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52651-0.025mg | |||
236-EG) using Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8274) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="EGFR phosphorylated at Y1197 was detected in immersion fixed A431 human epithelial carcinoma cell line treated (upper panel) or not (lower panel) with EGF (Catalog # 236-EG) using Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8274) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8274-SP | |||
![Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-212024941427.jpg "Immunocytochemistry/Immunofluorescence: EGFR Antibody (HL2541) - EGFRvIII, Mutant - Azide and BSA Free [NBP3-25446] - EGFR VIII antibody [HL2541] detects EGFR VIII protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR VIII stained by EGFR VIII antibody [HL2541] (NBP3-25446) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-261120251839316.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunohistochemical analysis.</br>Sample: EGFRvIII transfected 293T cell FFPE Cell Pellet Block.</br>Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:1000.</br>Blue: Fluoroshield with DAPI .</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunohistochemistry-paraffin-26112025195717.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.</br>Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.</br>Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.</br>Blue: Fluoroshield with DAPI .</p>*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunocytochemistry-immunofluorescence-26112025199124.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br><br>*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-2611202518224316.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:5000 and competitor's antibody (Highly Cited Antibody) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br><br>*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-western-blot-2611202518463713.jpg)
![EGFRvIII antibody [HL2541] detects EGFRvIII protein by immunofluorescent analysis.</br>Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.</br>Green: EGFRvIII stained by EGFRvIII antibody [HL2541] (NBP3-25446) diluted at 1:500 and competitor's antibody (highly cited competitor) diluted at 1:500.</br>Blue: Fluoroshield with DAPI .</p>*Competitor's antibody is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25446_rabbit-egfr-mab-hl2541-egfrviii-mutant-azide-bsa-free-immunocytochemistry-immunofluorescence-261120251818503.jpg)
![ELISA EGFR [p Tyr1068] [Biotin]](http://images.novusbio.com/fullsize/dyc3570-2_human-phospho-egfr-y1068-duoset-ic-elisa-2-plate-elisa-1592020123841.jpg "ELISA EGFR [p Tyr1068] [Biotin]")
NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. Negative staining shown in the lower panel with NCI-H1975 human lung epithelial cell line. View our protocol for 
![N/A EGFR [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_EGFR_DY231_ELISA_1808.jpg "N/A EGFR [Biotin]")
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining in human placenta and pancreas tissues . Corresponding EGFR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining in human placenta and pancreas tissues . Corresponding EGFR RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human testis shows no in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0009.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0006.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody [NBP1-84815] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NBP1-84815-img0008.jpg)
![Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29820241420932.jpg "Immunohistochemistry: EGFR Antibody (PD00-75) [NBP3-32255] - Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-EGFR antibody (NBP3-32255) at 1/800 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32255) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: EGFR Antibody (PD00-75) [NBP3-32255] - Immunocytochemistry analysis of A431 cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32255) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-2920241192377.jpg)
![Flow Cytometry: EGFR Antibody (PD00-75) [NBP3-32255] - Flow cytometric analysis of A431 cells labeling EGFR.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32255, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-29202416372141.jpg)
![Western Blot: EGFR Antibody (PD00-75) [NBP3-32255] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32255) at 1/2,000 dilution.<br/> Lane 1: HeLa cell lysate<br/> Lane 2: A431 cell lysate<br/> Lane 3: MDA-MB-468 cell lysate<br/> Lane 4: MCF7 cell lysate (low expression)<br/><br/> Lysates/proteins at 15 µg/Lane.<br/><br/> Predicted band size: 134 kDa<br/> Observed band size: 150 kDa<br/><br/> Exposure time: 21 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32255_rabbit-egfr-mab-pd00-75-3920248485012.jpg)
![Western blot analysis on different lysates with NBP3-32255 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MDA-MB-468 cell lysate Lane 4: MCF7 cell lysate (low expression) Lysates/proteins at 15 µg/Lane. Predicted band size: 134 kDa Observed band size: 150 kDa Exposure time: 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32255 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32255_-western-blot-639204594551774054.jpg)
![Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Western-Blot-NBP2-34243-img0006.jpg "Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).")
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0001.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0002.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Electropherogram image of the corresponding Simple Western lane. EGF R/ErbB1 antibody was used at 10 ug/ml dilution of A431 and HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0004.jpg)
![Protein Array: EGFR Antibody (H9B4) [NBP2-34243] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (H9B4) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Protein-Array-NBP2-34243-img0005.jpg)
AVI10494) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 190-230 kDa, respectively." class="big_thumb" />
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52651_rabbit-monoclonal-egfr-antibody-528-chimeric-74202522141227.png "Immunocytochemistry /Immunofluorescence: EGFR Antibody (528) - Chimeric - Azide and BSA Free [NBP2-52651] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton and stained with NBP2-52651 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52651, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
236-EG) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human Phospho-EGFR (Y1197) Monoclonal Antibody (Catalog # MAB8274) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # 
