| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75911SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75903SS | |||
236-EG) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y845) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3394), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y845) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y845) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3394), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y845) at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3394-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52671-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33068 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-724-0.025mg | |||
236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB89671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y1086) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB89671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y1086) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB89671-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03213-20ul | |||
236-EG) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB8967) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y1086) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB8967) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-EGFR (Y1086) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8967-SP | |||
![ELISA: EGFR Antibody (Matuzumab) [NBP2-75911] - ELISA Plate coated with human EGFR-Fc at a concentration of 5 ug/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using NBP2-75911. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.](http://images.novusbio.com/fullsize/EGFR-Antibody-Matuzumab-ELISA-NBP2-75911-img0003.jpg "ELISA: EGFR Antibody (Matuzumab) [NBP2-75911] - ELISA Plate coated with human EGFR-Fc at a concentration of 5 ug/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using NBP2-75911. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.")
![Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-Immunohistochemistry-NBP2-75903-img0002.jpg "Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.")
![ELISA: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - ELISA Plate coated with human EGFR-Fc at a concentration of 2.0 mg/ml. A 2-fold serial dilution from 100 to 0.1 ng/ml was performed using HRP labelled cetuximab human IgG1 (NBP2-75903).](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-ELISA-NBP2-75903-img0001.jpg)
236-EG; filled histogram) then stained with Rabbit Anti-Human Phospho-EGFR (Y845) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3394), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # 
NL004) and counterstained with DAPI (blue). View our protocol for 
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EGFR Antibody [NB100-596] - Section of human placenta. Antibody: Affinity purified rabbit anti-EGFR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/EGFR-Antibody-Immunohistochemistry-Paraffin-NB100-596-img0013.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: EGFR Antibody [NB100-596] - EGF R/ErbB1 Antibody [NB100-596] - Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-EGFR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0015.jpg)
![Western Blot: EGFR Antibody [NB100-596] - Detection of Human EGFR on HeLa whole cell lysate using NB100-596.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0005.jpg)
![Western Blot: EGFR Antibody [NB100-596] - Detection of Human EGFR by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody NB100-596 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/EGFR-Antibody-Western-Blot-NB100-596-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody (GFR450) [NBP2-29440] - Confocal Immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Immunocytochemistry-Immunofluorescence-NBP2-29440-img0006.jpg "Immunocytochemistry/Immunofluorescence: EGFR Antibody (GFR450) [NBP2-29440] - Confocal Immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).")
![Immunohistochemistry-Paraffin: EGFR Antibody (GFR450) [NBP2-29440] - Formalin-fixed, paraffin-embedded squamous cell carcinoma stained with EGFR Ab (GFR450).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Immunohistochemistry-Paraffin-NBP2-29440-img0003.jpg)
![Flow Cytometry: EGFR Antibody (GFR450) [NBP2-29440] - Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled EGFR antibody (GFR450).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Flow-Cytometry-NBP2-29440-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody (GFR450) [NBP2-29440] - Confocal Immunofluorescent analysis of A431 cells using AF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Immunocytochemistry-Immunofluorescence-NBP2-29440-img0005.jpg)
![Flow Cytometry: EGFR Antibody (GFR450) [NBP2-29440] - Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled EGFR Antibody (GFR450).](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Flow-Cytometry-NBP2-29440-img0008.jpg)
![Protein Array: EGFR Antibody (GFR450) [NBP2-29440] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (GFR450). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-GFR450-Protein-Array-NBP2-29440-img0009.jpg)
![Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Analysis using the FITC conjugate of NB600-724. Staining of A431 cells.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-ICR10-Flow-Cytometry-NB600-724-img0003.jpg "Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Analysis using the FITC conjugate of NB600-724. Staining of A431 cells.")
![Flow Cytometry: EGF R/ErbB1 Antibody (ICR10) [NB600-724] - Staining of A431 cells with RAT ANTI HUMAN EGF RECEPTOR (NB 600-724) visualised with F(ab')2 RABBIT ANTI RAT IgG: FITC secondary.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-Antibody-ICR10-Flow-Cytometry-NB600-724-img0001.jpg)
236-EG) using Rat Anti-Human EGFR Monoclonal Antibody (Catalog # MAB89671) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # 
236-EG) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-EGFR (Y1086) at approximately 197 kDa (as indicated) using 10 µg/mL of Rat Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB89671) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # 
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_EGF_R_DEGFR0_ELISA_655.jpg "N/A EGFR [HRP]")
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_EGF_R_DEGFR0_ELISA_656.jpg)
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize/elisa/EGF_R_DEGFR0_ELISA_80.jpg)
![IHC-P-EGFR Antibody [NBP3-03213] - Rat liver using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123725..jpg "IHC-P-EGFR Antibody [NBP3-03213] - Rat liver using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![IHC-P-EGFR Antibody [NBP3-03213] -Human placenta using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123838..jpg)
![IHC-P-EGFR Antibody [NBP3-03213] - Mouse heart using EGFR antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunohistochemistry-paraffin-2062023123952..jpg)
![Western Blot: EGFR Antibody [NBP3-03213] - analysis of A431, using EGFR Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-western-blot-611202384024..jpg)
![Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP3-03213] - Analysis of A-431 cells using EGFR Rabbit pAb at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-03213_rabbit-polyclonal-egfr-antibody-immunocytochemistry-immunofluorescence-611202384153..jpg)
236-EG) using Rabbit Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB8967) at a 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # 
236-EG) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-EGFR (Y1086) at approximately 199 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-EGFR (Y1086) Monoclonal Antibody (Catalog # MAB8967) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
