HAF007). Specific bands were detected for MAP3K8/Tpl2/COT at approximately 58 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MO7e human megakaryocytic leukemic cell line. PVDF Membrane was probed with 1 µg/mL of Human MAP3K8/Tpl2/COT Monoclonal Antibody (Catalog # MAB4586) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for MAP3K8/Tpl2/COT at approximately 58 and 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4586-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86569-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72589 | |||
![Western Blot: MAP3K8/Tpl2/COT Antibody [NBP1-62178] - 50-60 ug of rat midbrain protein. Primary antibody is diluted with 1:500 in DiluOBuffer. Apparent MW of MAP3K8 is 57 KDa.](http://images.novusbio.com/fullsize/MAP3K8-Tpl2-COT-Antibody-Western-Blot-NBP1-62178-img0001.jpg "Western Blot: MAP3K8/Tpl2/COT Antibody [NBP1-62178] - 50-60 ug of rat midbrain protein. Primary antibody is diluted with 1:500 in DiluOBuffer. Apparent MW of MAP3K8 is 57 KDa.")
![Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP1-62178] - 1:1000 dilution in IHC Blocking Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40x MAGNIFICATION. FFPE section.](http://images.novusbio.com/fullsize/MAP3K8-Tpl2-COT-Antibody-Immunohistochemistry-Paraffin-NBP1-62178-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MAP3K8-Tpl2-COT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86569-img0010.jpg "Immunocytochemistry/Immunofluorescence: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP1-86569] - Staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.](http://images.novusbio.com/fullsize/MAP3K8-Tpl2-COT-Antibody-Immunohistochemistry-Paraffin-NBP1-86569-img0009.jpg)
![Western Blot: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [HL2118] (NBP3-25556) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25556_rabbit-map3k8-tpl2-cot-mab-hl2118-azide-and-bsa-free-2120249393536.jpg "Western Blot: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [HL2118] (NBP3-25556) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K8 antibody [HL2118] (NBP3-25556) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25556_rabbit-map3k8-tpl2-cot-mab-hl2118-azide-and-bsa-free-212024940835.jpg)
![Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody (HL2118) - Azide and BSA Free [NBP3-25556] - MAP3K8 antibody [HL2118] detects MAP3K8 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. MAP3K8 stained by MAP3K8 antibody [HL2118] (NBP3-25556) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25556_rabbit-map3k8-tpl2-cot-mab-hl2118-azide-and-bsa-free-2120249351370.jpg)
![Western Blot: MAP3K8/Tpl2/COT Antibody (3D9) [NBP2-45741] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8.](http://images.novusbio.com/fullsize/MAP3K8-Tpl2-COT-Antibody-3D9-Western-Blot-NBP2-45741-img0001.jpg "Western Blot: MAP3K8/Tpl2/COT Antibody (3D9) [NBP2-45741] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8.")
![Western Blot: MAP3K8 Overexpression Lysate (Adult Normal) [NBL1-12859] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAP3K8.](http://images.novusbio.com/fullsize/MAP3K8-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12859-img0002.jpg "Western Blot: MAP3K8 Overexpression Lysate (Adult Normal) [NBL1-12859] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAP3K8.")
![Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP3-14395] - Immunohistochemistry of formalin-fixed, paraffin-embedded human colon after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/MAP3K8-Tpl2-COT Antibody-Immunohistochemistry-Paraffin-NBP3-14395-img0001.jpg "Immunohistochemistry-Paraffin: MAP3K8/Tpl2/COT Antibody [NBP3-14395] - Immunohistochemistry of formalin-fixed, paraffin-embedded human colon after heat-induced antigen retrieval.")
