| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26007 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26001 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25997 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25998 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25984 | |||
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-22620242232121.png "Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - RM150 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.")
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM150 at 0. 5 ug/mL, showed a band of histone H3 monomethylated at Lysine 9 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-2462024132634.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - Immunocytochemistry of HeLa cells, using Clone RM150 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-2462024124594.png)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody (RM150) - Azide and BSA Free [NBP3-26001] - ChIP performed on HeLa cells using Clone RM150 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-26001_rabbit-histone-h3-mab-rm150-azide-and-bsa-free-chromatin-immunoprecipitation-2462024225540..png)
![Western Blot: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using NBP2-61537, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys56-Antibody-RM179-Western-Blot-NBP2-61537-img0007.jpg "Western Blot: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using NBP2-61537, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys56) Rabbit mAb NBP2-61537 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys56-Antibody-RM179-Immunocytochemistry-Immunofluorescence-NBP2-61537-img0005.jpg)
![Immunohistochemistry: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - Immunohistochemistry staining of HepG2 cells using NBP2-61537.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys56-Antibody-RM179-Immunohistochemistry-NBP2-61537-img0006.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys56] Antibody (RM179) [NBP2-61537] - NBP2-61537 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys56-Antibody-RM179-Multiplex-Immunoassay-NBP2-61537-img0008.jpg)
![Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61535 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Western-Blot-NBP2-61535-img0007.jpg "Western Blot: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61535 at 0.5 ug/ml. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - Immunocytochemistry of HeLa cells, using monomethyl-Histone H3 (Lys9) Rabbit mAb NBP2-61535 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Immunocytochemistry-Immunofluorescence-NBP2-61535-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - ChIP performed on HeLa cells using H3K9me1 antibody (NBP2-61535). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Chromatin-Immunoprecipitation-NBP2-61535-img0009.jpg)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys9] Antibody (RM150) [NBP2-61535] - NBP2-61535 specifically reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys9-Antibody-RM150-Multiplex-Immunoassay-NBP2-61535-img0008.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys79] Antibody (RM181) [NBP2-61539] - NBP2-61539 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). It shows very slight cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys79-Antibody-RM181-Multiplex-Immunoassay-NBP2-61539-img0009.jpg "Multiplex Immunoassay: Histone H3 [Dimethyl Lys79] Antibody (RM181) [NBP2-61539] - NBP2-61539 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). It shows very slight cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.")
![Western Blot: Histone H3 [Dimethyl Lys79] Antibody (RM181) [NBP2-61539] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61539, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys79-Antibody-RM181-Western-Blot-NBP2-61539-img0008.jpg)
![Chromatin Immunoprecipitation (ChIP): Histone H3 [Dimethyl Lys79] Antibody (RM181) [NBP2-61539] - ChIP performed on HeLa cells using H3K79me2 antibody (NBP2-61539). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys79-Antibody-RM181-Chromatin-Immunoprecipitation-ChIP-NBP2-61539-img0010.jpg)
![Immunohistochemistry: Histone H3 [Dimethyl Lys79] Antibody (RM181) [NBP2-61539] - Immunohistochemistry staining of HepG2 cells using NBP2-61539.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys79-Antibody-RM181-Immunohistochemistry-NBP2-61539-img0007.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61534 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Western-Blot-NBP2-61534-img0007.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61534 at 0.5 ug/ml. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - Immunocytochemistry of HeLa cells, using NBP2-61534 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Immunocytochemistry-Immunofluorescence-NBP2-61534-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - ChIP performed on HeLa cells using 5ug NBP2-61534. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Chromatin-Immunoprecipitation-NBP2-61534-img0009.jpg)
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys9] Antibody (RM151) [NBP2-61534] - NBP2-61534 specifically reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-RM151-Multiplex-Immunoassay-NBP2-61534-img0008.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody (RM140) [NBP2-61533] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61533, showed a band of histone H3 monomethylated at Lysine 4 (K4me1) in HeLa cells. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys4-Antibody-RM140-Western-Blot-NBP2-61533-img0008.jpg "Western Blot: Histone H3 [Monomethyl Lys4] Antibody (RM140) [NBP2-61533] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using NBP2-61533, showed a band of histone H3 monomethylated at Lysine 4 (K4me1) in HeLa cells. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4] Antibody (RM140) [NBP2-61533] - Immunocytochemistry of HeLa cells, using Monomethyl-Histone H3 (Lys4) Rabbit mAb NBP2-61533 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys4-Antibody-RM140-Immunocytochemistry-Immunofluorescence-NBP2-61533-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Monomethyl Lys4] Antibody (RM140) [NBP2-61533] - ChIP performed on HeLa cells using H3K4me1 antibody (NBP2-61533). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys4-Antibody-RM140-Chromatin-Immunoprecipitation-NBP2-61533-img0010.jpg)
![Multiplex Immunoassay: Histone H3 [Monomethyl Lys4] Antibody (RM140) [NBP2-61533] - NBP2-61533 specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Monomethyl-Lys4-Antibody-RM140-Multiplex-Immunoassay-NBP2-61533-img0009.jpg)
![Western Blot: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K18ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-Western-Blot-NBP2-59217-img0001.jpg "Western Blot: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K18ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - ChIP assays were performed using human HeLa cells, treated with TSA, the antibody against H3K18ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-Chromatin-Immunoprecipitation-NBP2-59217-img0003.jpg)
![ELISA: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K18ac . The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,300.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18--Dimethyl-Arg17]-Antibody-ELISA-NBP2-59217-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys18, Dimethyl Arg17] Antibody [NBP2-59217] - HeLa cells were stained with the antibody against H3K18ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K18ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18-Dimethyl-Arg17]-Antibody-Immunofluorescence-NBP2-59217-img0004.jpg)
![Western Blot: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K36me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Western-Blot-NBP2-59211-img0001.jpg "Western Blot: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K36me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - Human osteosarcoma (U2OS) cells were stained with the antibody against H3K36me1 and with DAPI. Cells were fixed with 4% formaldehyde for 20 minutes and blocked with PBS/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H3K36me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 568 or with DAPI (right), which specifically labels DNA. Figure B, C, D and E: staining of the cells with the H3K36me1 antibody after incubation of the antibody with 2 ng/ul blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K36, respectively.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Immunofluorescence-NBP2-59211-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - ChIP assays were performed using human U2OS cells, the antibody against H3K36me1 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells and stringent washing conditions. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the constitutively expressed GAPDH gene and for the inactive gene MYOD. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59211-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys36] Antibody [NBP2-59211] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36me1, crude serum and Flow Through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:46,000.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys36]-Antibody-ELISA-NBP2-59211-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Cell cycle experiment using the antibody against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Western-Blot-NBP2-59212-img0006.jpg "Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Cell cycle experiment using the antibody against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis. Theoretical molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - ChIP assays were performed using human HeLa cells treated with colcemid, the antibody against H3K27me3S28p and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active gene GAPDH as a negative control, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3S28p is preferably present at inactive genes.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27-p-Ser28]-Antibody-Chromatin-Immunoprecipitation-NBP2-59212-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - Western blot was performed on histone extracts (15 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H3 (lane 2) using the antibody against H3K27me3S28p diluted 1:100 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Western-Blot-NBP2-59212-img0005.jpg)
![ELISA: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27me3S28p. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,300.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-ELISA-NBP2-59212-img0001.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27, p Ser28] Antibody [NBP2-59212] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K27me3S28p with peptides containing other modifications of histone H3 and H4 and the unmodified H3K27S28 sequence. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:2,000. The figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27--p-Ser28]-Antibody-Dot-Blot-NBP2-59212-img0004.jpg)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Western-Blot-NBP2-59294-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K9me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Immunofluorescence-NBP2-59294-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H3K9me3 and optimized PCR primer sets for qPCR. Chromatin was sheared with the antibody consisting of 1, 3 and 9 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-Chromatin-Immunoprecipitation-NBP2-59294-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys9] Antibody (C.15200154) [NBP2-59294] - Cross reactivity of the antibody directed against H3K9me2. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K9me2. The wells were coated with peptides containing the unmodified H3K9 as well as the mono-, di- and trimethylated H3K9 and the dimethylated H3K4 and H3K27. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Dimethyl-Lys9-Antibody-C-15200154-ELISA-NBP2-59294-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Western-Blot-NBP2-59295-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Immunofluorescence-NBP2-59295-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - ChIP assays were performed using human HeLa cells, the antibody against H3K27me3 and optimized PCR primer sets for qPCR. ChIP was performed with the AutoHistone ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH as negative controls, and for the coding regions of the inactive genes MYT1 and TSH2B as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me3 is preferably present at inactive genes.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-Chromatin-Immunoprecipitation-NBP2-59295-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys27] Antibody (C.15200181) [NBP2-59295] - Cross reactivity of the antibody directed against H3K27me3. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K27me3. The wells were coated with peptides containing the unmodified H3K27 region as well as the mono-, di- and trimethylated H3K27 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-C-15200181-ELISA-NBP2-59295-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me2 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59194-img0003.jpg "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - ChIP assays were performed using human HeLa cells, the antibody against H3K79me2 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).")
![Western Blot: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K79me2 diluted 1:200 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys79]-Antibody-Western-Blot-NBP2-59194-img0001.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - Analysis was performed to test the cross reactivity of the Diagenode antibody against H3K79me2 with peptides containing other modifications and unmodified sequences of histone H3. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure 4 shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys79]-Antibody-Dot-Blot-NBP2-59194-img0005.jpg)
![ELISA: Histone H3 [Dimethyl Lys79] Antibody [NBP2-59194] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K79me2 . The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:6,600.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys79]-Antibody-ELISA-NBP2-59194-img0002.jpg)