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![Western Blot: Histone H3 [ac Lys18] Antibody [NBP2-59218] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3R17me2(asym)K18ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Western-Blot-NBP2-59218-img0001.jpg "Western Blot: Histone H3 [ac Lys18] Antibody [NBP2-59218] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3R17me2(asym)K18ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys18] Antibody [NBP2-59218] - ChIP assays were performed using human HeLa cells, the antibody against H3R17me2(asym)K18ac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-59218-img0004.jpg)
![Dot Blot: Histone H3 [ac Lys18] Antibody [NBP2-59218] - To test the cross reactivity of the antibody against H3R17me2(asym)K18ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3R17K18. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Dot-Blot-NBP2-59218-img0002.jpg)
![ELISA: Histone H3 [ac Lys18] Antibody [NBP2-59218] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3R17me2(asym) K18ac. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:28,800.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-ELISA-NBP2-59218-img0003.jpg)
![Immunofluorescence: Histone H3 [ac Lys18] Antibody [NBP2-59218] - Mouse NIH3T3 cells were stained with the antibody against H3R17me2(asym)K18ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3R17me2(asym)K18ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys18]-Antibody-Immunofluorescence-NBP2-59218-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Chromatin-Immunoprecipitation-NBP2-44066-img0001.jpg "Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of Anti-Histone H3 R8me2a and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.")
![Western Blot: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate. Load: 30 Ug per lane. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Western-Blot-NBP2-44066-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg9] Antibody [NBP2-44066] - Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg9]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44066-img0002.jpg)
![Western Blot of Rabbit Anti-Histone H3 [Asym-dimethyl Arg9] Antibody Antibody. Lane 1: HeLa Histone Prep Lysate. 2. NIH-3T3 Prep Lysate. Lane 3: C. elegans embryo lysate. Load: 30 ug per lane. Primary antibody: Histone H3 [Asym-dimethyl Arg9] Antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15 kDa. Other band(s): None.](http://images.novusbio.com/fullsize/nbp2-44066_rabbit-polyclonal-histone-h3-asym-dimethyl-arg9-antibody-245202310564613.jpg)
![Chromatin Immunoprecipitation: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (NBP3-32401) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32401_rabbit-histone-h3-mab-je46-51-k27m-mutant-26820241525103.jpg "Chromatin Immunoprecipitation: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (NBP3-32401) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32401) at 1/1,000 dilution. Lane 1: 293T-OE-Histone H3 (mutated K27 M) cell lysate Lane 2: 293T-OE-Histone H3 cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 15 kDa Observed band size: 18 kDa Exposure time: 2 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32401) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32401_rabbit-histone-h3-mab-je46-51-k27m-mutant-278202415295586.jpg)
![Immunohistochemistry: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401] - Immunohistochemical analysis of paraffin-embedded 293T cells transfacted with Histone H3 / Histone H3 K27M mutant with Rabbit anti-Histone H3 antibody (NBP3-32401) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32401) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32401_rabbit-histone-h3-mab-je46-51-k27m-mutant-29820241492666.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401] - Immunocytochemistry analysis of HeLa cells transfacted with Histone H3 / Histone H3 labeling Histone H3 (K27M mutant ) with Rabbit anti-Histone H3 antibody (NBP3-32401) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32401) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32401_rabbit-histone-h3-mab-je46-51-k27m-mutant-2920241023420.jpg)
![Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-59225] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Western-Blot-NBP2-59225-img0001.jpg "Western Blot: Histone H3 [ac Lys27] Antibody [NBP2-59225] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K27ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys27] Antibody [NBP2-59225] - ChIP assays were performed using HeLa cells, the antibody against H3K27ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoter of the active ACTB and EIF4A2 genes, used as positive controls, and for the coding region of the inactive MYT1 and TSH2B genes, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27 acetylation is enriched at the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59225-img0003.jpg)
![ELISA: Histone H3 [ac Lys27] Antibody [NBP2-59225] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-ELISA-NBP2-59225-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys27] Antibody [NBP2-59225] - HeLa cells were stained with the antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys27]-Antibody-Immunofluorescence-NBP2-59225-img0004.jpg)
![Western Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - Western blot was performed on whole cell extracts from untreated HeLa cells (25 ug, lane 1), on histone extracts from HeLa cells treated with colcemid (15 ug, lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3T11p . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Western-Blot-NBP2-59274-img0001.jpg "Western Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - Western blot was performed on whole cell extracts from untreated HeLa cells (25 ug, lane 1), on histone extracts from HeLa cells treated with colcemid (15 ug, lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3T11p . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr11] Antibody [NBP2-59274] - ChIP assays were performed using human HeLa cells, treated with colcemid, the antibody against H3T11p and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Chromatin-Immunoprecipitation-NBP2-59274-img0004.jpg)
![Dot Blot: Histone H3 [p Thr11] Antibody [NBP2-59274] - To test the cross reactivity of the antibody against H3T11p, a Dot Blot analysis was performed with peptides containing other histone phosphorylations and the unmodified H3T11. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Dot-Blot-NBP2-59274-img0002.jpg)
![ELISA: Histone H3 [p Thr11] Antibody [NBP2-59274] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3T11p in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,700.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-ELISA-NBP2-59274-img0003.jpg)
![Immunofluorescence: Histone H3 [p Thr11] Antibody [NBP2-59274] - HeLa cells were treated with colcemid and stained with the antibody against H3T11p and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3T11p antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr11]-Antibody-Immunofluorescence-NBP2-59274-img0005.jpg)
![Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K79me3, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Western-Blot-NBP2-59205-img0007.jpg "Western Blot: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody against H3K79me3, diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - ChIP was performed with the antibody against H3K79me3 on sheared chromatin from 1 million HeLaS3 cells using a ChIP-seq kit. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for exon 2 of the inactive myoglobin gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K79me3 shows a preference for active promoters.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Chromatin-Immunoprecipitation-NBP2-59205-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K79me3 with peptides containing other histone modifications and the unmodified H3K79. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:100,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-Dot-Blot-NBP2-59205-img0005.jpg)
![ELISA: Histone H3 [Trimethyl Lys79] Antibody [NBP2-59205] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody directed against H3K79me3 in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,500.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys79]-Antibody-ELISA-NBP2-59205-img0006.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NBP2-59279] - Whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against H3K4ac, diluted 1:1,000 in TBSTween containing 5% BSA. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NBP2-59279-img0001.jpg "Western Blot: Histone H3 [ac Lys4] Antibody [NBP2-59279] - Whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against H3K4ac, diluted 1:1,000 in TBSTween containing 5% BSA. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody [NBP2-59279] - ChIP assays were performed using human HeLa cells, the antibody against H3K4ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and EIF4A2 promoters, used as positive controls, and for the HBB promoter and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NBP2-59279-img0003.jpg)
![ELISA: Histone H3 [ac Lys4] Antibody [NBP2-59279] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K4ac in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:197,500.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-ELISA-NBP2-59279-img0002.jpg)
![Immunofluorescence: Histone H3 [ac Lys4] Antibody [NBP2-59279] - HeLa cells were stained with the antibody against H3K4ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunofluorescence-NBP2-59279-img0004.jpg)
![Dot Blot: Histone H3 [ac Lys4] Antibody [NBP2-59279] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4ac with peptides containing other histone H3 and H4 modifications and the unmodified H3K4 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Dot-Blot-NBP2-59279-img0009.jpg)
![Western Blot: Histone H3 Antibody (1B1B2) [NBP2-59277] - Whole cell extracts from HeLa cells (40 ug, lane 1) and 1 ug of recombinant histone H3 and H4 (lane 2 and 3). The antibody was diluted 1:5000 in TBS-Tween containing 5% skim milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1B2-Western-Blot-NBP2-59277-img0006.jpg "Western Blot: Histone H3 Antibody (1B1B2) [NBP2-59277] - Whole cell extracts from HeLa cells (40 ug, lane 1) and 1 ug of recombinant histone H3 and H4 (lane 2 and 3). The antibody was diluted 1:5000 in TBS-Tween containing 5% skim milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody (1B1B2) [NBP2-59277] - HeLa cells were stained with the monoclonal antibody against H3 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labeled with the H3 antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1B2-Immunofluorescence-NBP2-59277-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 Antibody (1B1B2) [NBP2-59277] - ChIP assays were performed using human HeLa cells, the monoclonal antibody against H3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit for Histones, using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoters of the active GAPDH and EIF4A2 genes, and for the inactive MYOD1 gene and the Sat2 satellite repeat. Figure shows the recovery, expressed as a %input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1B2-Chromatin-Immunoprecipitation-NBP2-59277-img0005.jpg)
![Western Blot: Histone H3 Antibody (1B1B2) [NBP2-59277] - Western blot was performed on whole cell extracts (30 ug) from different celltypes (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using the antibody against H3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1B2-Western-Blot-NBP2-59277-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours and either Histone H3 (NBP3-32429) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-26820241525100.jpg "Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated 500ng/mL TSA for 4 hours and either Histone H3 (NBP3-32429) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Dot Blot: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Dot blot analysis of Histone H3 on different proteins with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Histone H3 (negative) Lane 2: Mono-Methyl-Histone H3 (Lys4) (negative) Lane 3: Di-Methyl-Histone H3 (Lys4) (negative) Lane 4: Tri-Methyl-Histone H3 (Lys4) (negative) Lane 5: Acetyl-Histone H3 (Lys4) (positive) Lane 6: Acetyl-Histone H3 (Lys9) (negative) Lane 7: Acetyl-Histone H3 (Lys27) (negative) Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 50 seconds.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-28820241459250.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Immunocytochemistry analysis of HeLa cells treated with or without 500ng/mL TSA for 4 hours labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-2920241192347.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody (PSH03-71) [NBP3-32429] - Western blot analysis of Histone H3 on different lysates with Rabbit anti-Histone H3 antibody (NBP3-32429) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32429_rabbit-histone-h3-ac-lys4-mab-psh03-71-3920248453547.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - ChIP performed on HeLa cells using Clone RM151 (5ug). Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-24620241332472.png "Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - ChIP performed on HeLa cells using Clone RM151 (5ug). Real-time PCR was performed using primers specific to the gene indicated.")
![Multiplex Immunoassay: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - RM151 specifically reacts to Histone H3 dimethylated at Lysine 9 (K9me2). No cross reactivity with non-modified Lysine 9 (H3 1-19), monomethylated Lysine 9 (K9me1), trimethylated Lysine 9 (K9me3), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-22620242311096.png)
![Western Blot: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM151 at 0.5 ug/mL, showed a band of histone H3 dimethylated at Lysine 9 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-22620242232120.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Dimethyl Lys9] Antibody (RM151) - Azide and BSA Free [NBP3-25998] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM151 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25998_rabbit-histone-h3-mab-rm151-azide-and-bsa-free-2462024124593.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2462024133242.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - RM156 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2462024132628.png)
![Western Blot: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with Sodium Butyrate (+), using RM156 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-2262024231100.png)
![Sandwich ELISA: Histone H3 [ac Lys79] Antibody (RM156) - Azide and BSA Free [NBP3-25990] - Sandwich ELISA against acetylated histone H3 at Lys 79 using Clone RM156 (5 ug/mL) as the capture antibody and another biotinylated Histone H3 (1 ug/mL) as the detection antibody.](http://images.novusbio.com/fullsize/nbp3-25990_rabbit-histone-h3-mab-rm156-azide-and-bsa-free-22620242311033.png)
![Multiplex Immunoassay: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-22620242311057.png "Multiplex Immunoassay: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - RM179 specifically reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl), acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.")
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM179 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-24620241245921.png)
![Immunohistochemistry-Paraffin: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Immunohistochemistry staining of HepG2 cells using Clone RM179.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-2462024132630.png)
![Western Blot: Histone H3 [ac Lys56] Antibody (RM179) - Azide and BSA Free [NBP3-26007] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant Histone H3.3, using RM179 at 1 ug/mL, showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa.](http://images.novusbio.com/fullsize/nbp3-26007_rabbit-histone-h3-mab-rm179-azide-and-bsa-free-22620242311024.png)