| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1254AF405 | |||
| Alexa Fluor 488 | NB21-1254AF488 | |||
| Alexa Fluor 647 | NB21-1254AF647 | |||
| Alexa Fluor 700 | NB21-1254AF700 | |||
| Biotin | NB21-1254B | |||
| DyLight 405 | NB21-1254V | |||
| DyLight 488 | NB21-1254G | |||
| DyLight 550 | NB21-1254R | |||
| DyLight 650 | NB21-1254C | |||
| HRP | NB21-1254H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1254SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1001AF405 | |||
| Alexa Fluor 488 | NB21-1001AF488 | |||
| Alexa Fluor 647 | NB21-1001AF647 | |||
| Alexa Fluor 700 | NB21-1001AF700 | |||
| Biotin | NB21-1001B | |||
| DyLight 405 | NB21-1001V | |||
| DyLight 488 | NB21-1001G | |||
| DyLight 550 | NB21-1001R | |||
| DyLight 650 | NB21-1001C | |||
| HRP | NB21-1001H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1001SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1038V | |||
| FITC | NB21-1038F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26019 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25982 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-25990 | |||
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3.1, H3.3 and H4 (lane 3, 4, 5, 6 and 7, respectively) using the antibody against H3K4me3. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Western-Blot-NBP2-59186-img0002.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Western blot was performed on whole cell (40 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3.1, H3.3 and H4 (lane 3, 4, 5, 6 and 7, respectively) using the antibody against H3K4me3. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - HeLa cells were stained with the antibody against H3K4me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4me3 antibody (left) diluted 1:100 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59186-img0001.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3 modifications and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me3 modification. At higher concentrations some H3K4me2 is also precipitated.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59186-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - ChIP assays were performed using human HeLa cells, the Ab against H3K4me3 and PCR primers for qPCR. ChIP was performed using sheared chromatin from 1 million cells. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as positive controls, and for the inactive MYOD1 gene, used as negative control. The graph shows the recovery, expressed as a % of input (the relative amount of IP DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59186-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NBP2-59186] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4me3 with peptides containing other modifications and unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:2,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Dot-Blot-NBP2-59186-img0003.jpg)
![Dot Blot: Histone H3 [ac Lys36] Antibody [NB21-1254] - Analysis of H3 K36ac in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Dot-Blot-NB21-1254-img0001.jpg "Dot Blot: Histone H3 [ac Lys36] Antibody [NB21-1254] - Analysis of H3 K36ac in picomoles of peptide.")
![Western Blot: Histone H3 [ac Lys36] Antibody [NB21-1254] - Analysis of Histone H3 K36ac in C. elegans embryo lysate. Observed molecular weight is ~16 kDa](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys36]-Antibody-Western-Blot-NB21-1254-img0002.jpg)
![Western Blot: Histone H3 [Monomethyl Arg2] Antibody [NB21-1001] - Analysis of Histone H3 [Monomethyl Arg2] in C. elegans embryo lysate. Observed molecular weight is ~14 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Arg2]-Antibody-Western-Blot-NB21-1001-img0007.jpg "Western Blot: Histone H3 [Monomethyl Arg2] Antibody [NB21-1001] - Analysis of Histone H3 [Monomethyl Arg2] in C. elegans embryo lysate. Observed molecular weight is ~14 kDa.")
![Western Blot: Histone H3 [Monomethyl Arg2] Antibody [NB21-1001] - Analysis of Histone H3 [Monomethyl Arg2] in HeLa histone prep lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Arg2]-Antibody-Western-Blot-NB21-1001-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4, p Thr6] Antibody [NB21-1038] - Antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--p-Thr6]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1038-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4, p Thr6] Antibody [NB21-1038] - Antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.")
![Western Blot: Histone H3 [Dimethyl Lys4, p Thr6] Antibody [NB21-1038] - Analysis of HeLa histone prep lysate (A), NIH 3T3 histone prep lysate (B), and C. elegans histone prep lysate (C) using Histone H3 [Dimethyl Lys4, p Thr6] antibody at 2 ug/mL. Observed molecular weight is ~15 kDa](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4--p-Thr6]-Antibody-Western-Blot-NB21-1038-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-2462024132610.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM130 (red). Actin filaments have been labeled with fluorescein phalloidin (green).")
![Multiplex Immunoassay: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-2462024132621.png)
![Western Blot: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using RM130 at 0.5 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-2462024133241.png)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - ChIP performed on HeLa cells using RM130, 5ug. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-226202422321239.png)
![Multiplex Immunoassay: Histone H3 [ac Lys14] Antibody (RM130) - Azide and BSA Free [NBP3-25982] - RM130 specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.](http://images.novusbio.com/fullsize/nbp3-25982_rabbit-histone-h3-mab-rm130-azide-and-bsa-free-24620241245915.png)
![Western Blot: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3). Using NBP2-61546, showed a band of Histone H3 phosphorylated at threonine 3 in HeLa cells. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Western-Blot-NBP2-61546-img0007.jpg "Western Blot: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3). Using NBP2-61546, showed a band of Histone H3 phosphorylated at threonine 3 in HeLa cells. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunocytochemistry of HeLa cells using NBP2-61546 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Immunocytochemistry-Immunofluorescence-NBP2-61546-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunocytochemistry of HeLa cells using NBP2-61546 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-p-Thr3-Antibody-RM159-Immunocytochemistry-Immunofluorescence-NBP2-61546-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Thr3] Antibody (RM159) [NBP2-61546] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using NBP2-61546 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-61546_rabbit-monoclonal-histone-h3-p-thr3-antibody-rm159-131020231935490.png)
![Western Blot: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using NBP2-61532. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys14-Antibody-RM130-Western-Blot-NBP2-61532-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) or, using NBP2-61532. Observed molecular weight is ~17 kDa.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61532 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys14-Antibody-RM130-Immunocytochemistry-Immunofluorescence-NBP2-61532-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - ChIP performed on HeLa cells using NBP2-61532. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys14-Antibody-RM130-Chromatin-Immunoprecipitation-NBP2-61532-img0011.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - NBP2-61532 specifically reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys14-Antibody-RM130-Multiplex-Immunoassay-NBP2-61532-img0009.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys14] Antibody (RM130) [NBP2-61532] - The binding specificity of NBP2-61532 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys14-Antibody-RM130-Multiplex-Immunoassay-NBP2-61532-img0010.jpg)
![Western Blot: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with Sodium Butyrate (+), using NBP2-61497 at 1 ug/ml. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys79-Antibody-RM156-Western-Blot-NBP2-61497-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - Western Blot of acid extracts from HeLa cells untreated (-) or treated with Sodium Butyrate (+), using NBP2-61497 at 1 ug/ml. Observed molecular weight is ~16 kDa.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61497 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys79-Antibody-RM156-Immunocytochemistry-Immunofluorescence-NBP2-61497-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using 5 ug NBP2-61497. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys79-Antibody-RM156-Chromatin-Immunoprecipitation-NBP2-61497-img0007.jpg)
![ELISA: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - Sandwich ELISA against acetylated histone H3 using NBP2-61497 at 5 ug/ml as the capture antibody and NBP2-61547 at 1 ug/ml as the detection antibody.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys79-Antibody-RM156-ELISA-NBP2-61497-img0003.jpg)
![Multiplex Immunoassay: Histone H3 [ac Lys79] Antibody (RM156) [NBP2-61497] - NBP2-61497 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-ac-Lys79-Antibody-RM156-Multiplex-Immunoassay-NBP2-61497-img0005.jpg)
![Western Blot: Histone H3 [Methyl Lys4] Antibody [NBP2-59188] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me1 . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys4]-Antibody-Western-Blot-NBP2-59188-img0001.jpg "Western Blot: Histone H3 [Methyl Lys4] Antibody [NBP2-59188] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K4me1 . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Methyl Lys4] Antibody [NBP2-59188] - HeLa cells were stained with the antibody against H3K4me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys4]-Antibody-Immunofluorescence-NBP2-59188-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys4] Antibody [NBP2-59188] - Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me1, H3K27me1, H3K36me1, H4K20me1 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me1 modification.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59188-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys4] Antibody [NBP2-59188] - ChIP was performed with the antibody against H3K4me1 on sheared chromatin from 500,000 HeLaS3 cells using a ChIP-seq kit. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 genes, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NBP2-59188-img0005.jpg)
![Dot Blot: Histone H3 [Methyl Lys4] Antibody [NBP2-59188] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4me1 with peptides containing other modifications or unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys4]-Antibody-Dot-Blot-NBP2-59188-img0004.jpg)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-C-15200152-Western-Blot-NBP2-59289-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K4me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-C-15200152-Immunofluorescence-NBP2-59289-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - ChIP assays were performed using human HeLa cells, the antibody against H3K4me3 and PCR primer pairs for qPCR. ChIP was performed with a Histone ChIP-seq kit, using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the constitutively expressed GAPDH and c-fos genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-C-15200152-Chromatin-Immunoprecipitation-NBP2-59289-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys4] Antibody (C.15200152) [NBP2-59289] - Cross reactivity of the antibody directed against H3K4me3. To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K4me3. The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys4-Antibody-C-15200152-ELISA-NBP2-59289-img0002.jpg)
![Western Blot: Histone H3 [Dimethyl Lys27] Antibody [NBP2-59193] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys27]-Antibody-Western-Blot-NBP2-59193-img0001.jpg "Western Blot: Histone H3 [Dimethyl Lys27] Antibody [NBP2-59193] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys27] Antibody [NBP2-59193] - HeLa cells were stained with the antibody against H3K27me2 and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27me2 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys27]-Antibody-Immunofluorescence-NBP2-59193-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys27] Antibody [NBP2-59193] - ChIP assays were performed using human HeLa cells, the antibody against H3K27me2 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the promoter of the inactive HBB and the coding region of the inactive MYOD1 genes, used as positive controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me2 is preferably present at silent genes.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59193-img0003.jpg)
![ELISA: Histone H3 [Dimethyl Lys27] Antibody [NBP2-59193] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27me2. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:480,000.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys27]-Antibody-ELISA-NBP2-59193-img0002.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys27] Antibody [NBP2-59193] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K27me2 with peptides containing other modifications of histone H3 and H4 and the unmodified sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:50,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys27]-Antibody-Dot-Blot-NBP2-59193-img0006.jpg)
![Western Blot: Histone H3 [Methyl Lys27] Antibody [NBP2-59192] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys27]-Antibody-Western-Blot-NBP2-59192-img0001.jpg "Western Blot: Histone H3 [Methyl Lys27] Antibody [NBP2-59192] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody against H3K27me1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Methyl Lys27] Antibody [NBP2-59192] - Human osteosarcoma (U2OS) cells were stained with Histone H3 [Methyl Lys27] Antibody and with DAPI. Cells were fixed with 4% formaldehyde for 20' and blocked with PBS/TX-100 containing 5% normal goat serum. (A): cells were immunofluorescently labeled with the Histone H3 [Methyl Lys27] Antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. (B), (C), (D), (E): staining of the cells with the Histone H3 [Methyl Lys27] Antibody after incubation of the antibody with 2 ng/ul blocking peptide containing the unmodified and the mono-, di- and trimethylated Histone H3 [Methyl Lys27], respectively.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59192-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Methyl Lys27] Antibody [NBP2-59192] - ChIP assays were performed using human HeLa cells, the antibody against H3K27me1 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the active gene GAPDH used as a negative and a positive control target, respectively. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NBP2-59192-img0003.jpg)
![ELISA: Histone H3 [Methyl Lys27] Antibody [NBP2-59192] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K27me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:32,900.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys27]-Antibody-ELISA-NBP2-59192-img0002.jpg)
![Dot Blot: Histone H3 [Methyl Lys27] Antibody [NBP2-59192] - A Dot Blot analysis was performed to test the cross reactivity of against Histone H3 [Methyl Lys27] Antibody with peptides containing other modifications and unmodified sequences of Histone H3 and H4. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. This image shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Methyl-Lys27]-Antibody-Dot-Blot-NBP2-59192-img0004.jpg)