Products Related to Gene-ID: 11148

8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841R). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050)." class="big_lightbox"> 8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841R). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050)." alt="In a functional flow cytometry test, biotinylated recombinant human TMIGD2/CD28H (10 ng/mL, Catalog # 8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841R). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050)." class="big_thumb" />

F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

8316-TR) is coated at 2 µg/mL (100 µL/well), Recombinant Cynomolgus B7-H7/HHLA2 (Catalog # 10109-B7) binds with an ED₅₀ of 0.2-1.2 µg/mL" class="big_lightbox"> 8316-TR) is coated at 2 µg/mL (100 µL/well), Recombinant Cynomolgus B7-H7/HHLA2 (Catalog # 10109-B7) binds with an ED₅₀ of 0.2-1.2 µg/mL" alt="When Recombinant Human TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR) is coated at 2 µg/mL (100 µL/well), Recombinant Cynomolgus B7-H7/HHLA2 (Catalog # 10109-B7) binds with an ED₅₀ of 0.2-1.2 µg/mL" class="big_thumb" />

8316-TR) is immobilized at 2.5 μg/mL (100 μL/well), Recombinant Human B7 H7/HHLA2 His-tag (Catalog # 10475-B7) that produces a 50% optimal binding response is found to be 0.8-4.8 μg/mL." class="big_lightbox"> 8316-TR) is immobilized at 2.5 μg/mL (100 μL/well), Recombinant Human B7 H7/HHLA2 His-tag (Catalog # 10475-B7) that produces a 50% optimal binding response is found to be 0.8-4.8 μg/mL." alt="When Recombinant Human TMIGD2/CD28H (8316-TR) is immobilized at 2.5 μg/mL (100 μL/well), Recombinant Human B7 H7/HHLA2 His-tag (Catalog # 10475-B7) that produces a 50% optimal binding response is found to be 0.8-4.8 μg/mL." class="big_thumb" />

2 μg/lane of Recombinant Human B7-H7/HHLA2 His-tag Protein (Catalog # 10475-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver stain, showing bands at 55-65 kDa." class="big_lightbox"> 2 μg/lane of Recombinant Human B7-H7/HHLA2 His-tag Protein (Catalog # 10475-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver stain, showing bands at 55-65 kDa." class="big_thumb" />

G-102-C)." class="big_lightbox"> G-102-C)." alt="Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.075-0.75 µg/mL in the presence of Goat Anti-Human IgG Fc Polyclonal Antibody (Catalog # G-102-C)." class="big_thumb" />

8316-TR) with an ED₅₀ of < 100 ng/mL." class="big_lightbox"> 8316-TR) with an ED₅₀ of < 100 ng/mL." alt="Biotinylated Recombinant Human B7-H7/HHLA2 Fc Chimera binds to Recombinant TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR) with an ED₅₀ of < 100 ng/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="B7-H7/HHLA2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-HumanB7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB8084) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />