HAF109). A specific band was detected for RACK1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, A20 mouse B cell lymphoma cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RACK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3434) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RACK1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3434-SP | |||
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for ![Immunocytochemistry/Immunofluorescence: RACK1/GNB2L1 Antibody [NBP1-83356] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RACK1-GNB2L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83356-img0006.jpg "Immunocytochemistry/Immunofluorescence: RACK1/GNB2L1 Antibody [NBP1-83356] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Western Blot: GNB2L1 Antibody [NBP1-58959] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/GNB2L1-Antibody-Western-Blot-NBP1-58959-img0002.jpg "Western Blot: GNB2L1 Antibody [NBP1-58959] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: GNB2L1 Antibody [NBP1-58959] - Human small intestine tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/GNB2L1-Antibody-Immunohistochemistry-Paraffin-NBP1-58959-img0004.jpg)
![Western Blot: RACK1/GNB2L1 Antibody (S02-1K6) [NBP3-14967] - Western blot detection of RACK1/GNB2L1 in C6, 3T3, Hela cell lysates using RACK1/GNB2L1 Rabbit mAb (1:1000 diluted). Predicted band size: 35kDa. Observed band size: 35kDa.](http://images.novusbio.com/fullsize/RACK1-GNB2L1-Antibody-S02-1K6-Western-Blot-NBP3-14967-img0001.jpg "Western Blot: RACK1/GNB2L1 Antibody (S02-1K6) [NBP3-14967] - Western blot detection of RACK1/GNB2L1 in C6, 3T3, Hela cell lysates using RACK1/GNB2L1 Rabbit mAb (1:1000 diluted). Predicted band size: 35kDa. Observed band size: 35kDa.")
![Immunohistochemistry-Paraffin: RACK1/GNB2L1 Antibody (S02-1K6) [NBP3-14967] - Immunohistochemistry of RACK1/GNB2L1 in paraffin-embedded Human breast cancer tissue using RACK1/GNB2L1 Rabbit mAb at dilution 1/20](http://images.novusbio.com/fullsize/RACK1-GNB2L1-Antibody-S02-1K6-Immunohistochemistry-Paraffin-NBP3-14967-img0002.jpg)
![Western Blot: RACK1/GNB2L1 Antibody (7B6J4) [NBP3-16277] - Western blot analysis of extracts of various cell lines, using RACK1/GNB2L1 Rabbit mAb (NBP3-16277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/RACK1-GNB2L1-Antibody-7B6J4-Western-Blot-NBP3-16277-img0001.jpg "Western Blot: RACK1/GNB2L1 Antibody (7B6J4) [NBP3-16277] - Western blot analysis of extracts of various cell lines, using RACK1/GNB2L1 Rabbit mAb (NBP3-16277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: RACK1/GNB2L1 Antibody (7B6J4) [NBP3-16277] - Western blot analysis of extracts of various cell lines, using RACK1/GNB2L1 Rabbit mAb (NBP3-16277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/RACK1-GNB2L1-Antibody-7B6J4-Western-Blot-NBP3-16277-img0002.jpg)
![Western Blot: GNB2L1 Overexpression Lysate (Adult Normal) [NBL1-11166] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GNB2L1.](http://images.novusbio.com/fullsize/GNB2L1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11166-img0002.jpg "Western Blot: GNB2L1 Overexpression Lysate (Adult Normal) [NBL1-11166] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GNB2L1.")
![Western Blot: RACK1/GNB2L1 Antibody [NBP3-12416] - A. Thaliana extract. 1:500 antibody dilution in diluobuffer.](http://images.novusbio.com/fullsize/RACK1-GNB2L1-Antibody-Western-Blot-NBP3-12416-img0001.jpg "Western Blot: RACK1/GNB2L1 Antibody [NBP3-12416] - A. Thaliana extract. 1:500 antibody dilution in diluobuffer.")
![SDS-Page: Recombinant Human RACK1/GNB2L1 His Protein [NBP1-78874] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human RACK1-GNB2L1 His Protein-SDS-Page-NBP1-78874-img0002.jpg "SDS-Page: Recombinant Human RACK1/GNB2L1 His Protein [NBP1-78874] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
