Species: All-NA
Applications: Func
Description
MALP-2, TLR6 and TLR2 ligand sticks avidly to glass or plastic. Since it is active at very low concentrations, i.e. at high dilutions, these low amounts of material tend to become adsorbed to pipette tips and plastic or glass containers. To avoid this, prepare several dilution steps of the MALP-2, TLR6 and TLR2 ligand stock solution with medium containing 5% autologous serum or buffers with 2% HSA. The presence of 50mM octyl glucoside in the first dilution step is often beneficial and gives rise to higher activity. Avoid small volumes in large vessels. Do not filter.Because of the ester-bound fatty acids, MALP-2, TLR6 and TLR2 ligand is sensitive to alkali or acid. The medium should be preconditioned in the CO2 incubator in order to ensure that the pH does not become alkaline. Add cells in preconditioned medium as soon as possible and incubate.
Bioinformatics
| Alternate Names |
- Macrophage-activating lipopeptide-2
- MALP 2
- MALP-2, TLR6 & TLR2 ligand
- S-[2,3-bis(Palmityloxy)-(2R)-propyl-cysteinyl-GNNDESNISFKEK]
- TLR activator MALP2
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