Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/mL of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.](http://images.novusbio.com/fullsize/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0003.jpg "MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - 293 cells were transfected with pCMV/TLR2-6 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 50 and 100 ng/mL of MALP2 as added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit.")
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Evaluation of the MALP2 ligand activity on TLR2/NF-kB SEAPorter HEK 293 cell line.Cell line is a stably co-transfected cell line that expresses full-length human Toll-like receptor 2 (TLR2) and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element. Cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amounts of MALP2 for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: MALP2 specifically activated the TLR2/6-depedent NF-kB/SEAP reporter cells in a dose dependent manner. Note: HEK 293 cells endogenously express TLR6.](http://images.novusbio.com/fullsize/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0004.jpg)
![MALP-2, TLR6 and TLR2 ligand [NBP2-26219] - Differentiated human macrophages were treated with or without MALP-2 (100 ng/mL), and the release of IL-6 in culture supernatants of cells was measured by ELISA kit. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MALP-2--TLR6-and-TLR2-ligand-NBP2-26219-img0005.jpg)