Autophagy/Cytotoxicity Dual Staining Kit

Functional: Autophagy/Cytotoxicity Dual Staining Other Kit [KA1299] - Tamoxifen increases autophagy but not cell death in HepG2 cells as measured by fluorescence microscopy. Panel A : MDC staining of HepG2 cells treated with vehicle. There is a basal level of autophagy, indicated by faint silver dot staining of autophagic vacuoles. Panel B : Propidium iodide staining of HepG2 cells treated with vehicle. There are few dead cells with only background staining of propidium iodide. Panel C : MDC staining of HepG2 cells treated with 10 uM Tamoxifen. Panel D : Propidium iodide staining of HepG2 cells treated with 10 uM Tamoxifen, showing a similar staining pattern to that of cells treated with vehicle

• Reactivity: I
• Applications: Flow, Func, ICC/IF
• Suitable Sample Type: Cultured Cells

Autophagy/Cytotoxicity Dual Staining Kit Summary

• Description: Autophagy/Cytotoxicity Dual Staining Kit is a fluorescence-labeled method for screening autophagy and cytotoxicity at the cellular level.
• Sample Volume: 5 x 10^4 cells per well. All Treatments conditions should be assayed in duplicate at minimum.
• Kit Type: Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence

Reactivity Notes

Reacts with Frugiperda.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Autophagy/Cytotoxicity Dual Staining Kit (KA1299)(1)

Publication using KA1299

• Teng CY, van Oers MM, Wu TY. Additive effect of calreticulin and translation initiation factor eIF4E on secreted protein production in the baculovirus expression system. Appl Microbiol Biotechnol. 2013-10-01 [PMID: 23900798]