Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93343-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92443-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16056-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35343-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35445-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94325-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35590-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35170-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38158-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04468-20ul | |||
![Western Blot: RNF149 Antibody - Azide and BSA Free [NBP2-93343] - Western blot analysis of lysates from Mouse stomach using RNF149 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-93343_rabbit-polyclonal-rnf149-antibody-42202514264497.jpg "Western Blot: RNF149 Antibody - Azide and BSA Free [NBP2-93343] - Western blot analysis of lysates from Mouse stomach using RNF149 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: RNF149 Antibody - Azide and BSA Free [NBP2-93343] - Immunofluorescence analysis of NIH/3T3 cells using RNF149 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93343_rabbit-polyclonal-rnf149-antibody-422025143043.jpg)
![Western Blot: ATP5H Antibody [NBP2-92443] - Analysis of extracts of various cell lines, using ATP5H at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Western-Blot-NBP2-92443-img0001.jpg "Western Blot: ATP5H Antibody [NBP2-92443] - Analysis of extracts of various cell lines, using ATP5H at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-92443] - Immunohistochemistry of paraffin-embedded mouse kidney using ATP5H Rabbit pAb (NBP2-92443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-92443-img0004.jpg)
![Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-92443] - Immunohistochemistry of paraffin-embedded rat kidney using ATP5H Rabbit pAb (NBP2-92443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATP5H-Antibody-Immunohistochemistry-Paraffin-NBP2-92443-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5H Antibody - BSA Free [NBP2-92443] - Confocal immunofluorescence analysis of U2OS cells using ATP5H Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92443_rabbit-polyclonal-atp5h-antibody-4220251429055.jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [NBP3-16596] - Immunohistochemistry of paraffin-embedded mouse kidney using Angiopoietin-like Protein 3/ANGPTL3 Rabbit mAb (NBP3-16596) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Angiopoietin-like-Protein-3-ANGPTL3-Antibody-4W9A7-Immunohistochemistry-Paraffin-NBP3-16596-img0001.jpg "Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [NBP3-16596] - Immunohistochemistry of paraffin-embedded mouse kidney using Angiopoietin-like Protein 3/ANGPTL3 Rabbit mAb (NBP3-16596) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [NBP3-16596] - Confocal imaging of paraffin-embedded Rat liver using ANGPTL3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16596_rabbit-angiopoietin-like-protein-3-angptl3-mab-4w9a7-immunohistochemistry-paraffin-198202485115..jpg)
![Immunohistochemistry-Paraffin: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [NBP3-16596] - Confocal imaging of paraffin-embedded Mouse liver using ANGPTL3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16596_rabbit-angiopoietin-like-protein-3-angptl3-mab-4w9a7-immunohistochemistry-paraffin-198202485220..jpg)
![Western Blot: Angiopoietin-like Protein 3/ANGPTL3 Antibody (4W9A7) [Angiopoietin-like Protein 3/ANGPTL3] - Western blot analysis of various lysates using Angiopoietin-like Protein 3/ANGPTL3 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16596_rabbit-angiopoietin-like-protein-3-angptl3-mab-4w9a7-52202517213530.jpg)
![Western Blot: DYNC1LI2 Antibody [NBP3-16056] - Western blot analysis of extracts of various cell lines, using DYNC1LI2 antibody (NBP3-16056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DYNC1LI2-Antibody-Western-Blot-NBP3-16056-img0005.jpg "Western Blot: DYNC1LI2 Antibody [NBP3-16056] - Western blot analysis of extracts of various cell lines, using DYNC1LI2 antibody (NBP3-16056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: DYNC1LI2 Antibody [NBP3-16056] - Immunohistochemistry of paraffin-embedded rat kidney using DYNC1LI2 Rabbit pAb (NBP3-16056) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DYNC1LI2-Antibody-Immunohistochemistry-Paraffin-NBP3-16056-img0004.jpg)
![Immunohistochemistry-Paraffin: DYNC1LI2 Antibody [NBP3-16056] - Immunohistochemistry of paraffin-embedded human colon carcinoma using DYNC1LI2 Rabbit pAb (NBP3-16056) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DYNC1LI2-Antibody-Immunohistochemistry-Paraffin-NBP3-16056-img0001.jpg)
![Immunohistochemistry-Paraffin: DYNC1LI2 Antibody [NBP3-16056] - Immunohistochemistry of paraffin-embedded human esophageal cancer using DYNC1LI2 Rabbit pAb (NBP3-16056) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DYNC1LI2-Antibody-Immunohistochemistry-Paraffin-NBP3-16056-img0002.jpg)
![Immunohistochemistry-Paraffin: DYNC1LI2 Antibody [NBP3-16056] - Immunohistochemistry of paraffin-embedded mouse spinal cord using DYNC1LI2 Rabbit pAb (NBP3-16056) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DYNC1LI2-Antibody-Immunohistochemistry-Paraffin-NBP3-16056-img0003.jpg)
![Western Blot: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Western blot analysis of various lysates using alcohol dehydrogenase 5 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35343_rabbit-alcohol-dehydrogenase-5-pab-912202414201366.jpg "Western Blot: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Western blot analysis of various lysates using alcohol dehydrogenase 5 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Immunofluorescence analysis of NIH/3T3 cells using alcohol dehydrogenase 5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35343_rabbit-alcohol-dehydrogenase-5-pab-912202414123827.jpg)
![Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Immunofluorescence analysis of PC-12 cells using alcohol dehydrogenase 5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35343_rabbit-alcohol-dehydrogenase-5-pab-31120251704741.jpg)
![Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Immunofluorescence analysis of HepG2 cells using alcohol dehydrogenase 5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35343_rabbit-alcohol-dehydrogenase-5-pab-3112025177013.jpg)
![Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Immunofluorescence analysis of HeLa cells using alcohol dehydrogenase 5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35343_rabbit-alcohol-dehydrogenase-5-pab-3112025173167.jpg)
![Western Blot: SNW1 Antibody [NBP3-35445] - Western blot analysis of various lysates using SNW1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35445_rabbit-snw1-pab-101220241233837.jpg "Western Blot: SNW1 Antibody [NBP3-35445] - Western blot analysis of various lysates using SNW1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunohistochemistry: SNW1 Antibody [NBP3-35445] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SNW1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35445_rabbit-snw1-pab-1012202411531072.jpg)
![Immunohistochemistry: SNW1 Antibody [NBP3-35445] - Immunohistochemistry analysis of paraffin-embedded Rat brain using SNW1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35445_rabbit-snw1-pab-101220241214437.jpg)
![Immunocytochemistry/ Immunofluorescence: SNW1 Antibody [NBP3-35445] - Immunofluorescence analysis of U-2 OS cells using SNW1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35445_rabbit-snw1-pab-101220241252838.jpg)
![Western Blot: Thyroid receptor-interacting protein 12 Antibody [NBP2-94325] - Analysis of extracts of various cell lines, using Thyroid receptor-interacting protein 12 . Exposure time: 30s.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Western-Blot-NBP2-94325-img0004.jpg "Western Blot: Thyroid receptor-interacting protein 12 Antibody [NBP2-94325] - Analysis of extracts of various cell lines, using Thyroid receptor-interacting protein 12 . Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Thyroid receptor-interacting protein 12 Antibody [NBP2-94325] - Rat testis using TRIP12 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-Paraffin-NBP2-94325-img0003.jpg)
![Immunohistochemistry-Paraffin: Thyroid receptor-interacting protein 12 Antibody [NBP2-94325] - Human colon using TRIP12 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-Paraffin-NBP2-94325-img0001.jpg)
![Immunohistochemistry-Paraffin: Thyroid receptor-interacting protein 12 Antibody [NBP2-94325] - Mouse testis using TRIP12 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Immunohistochemistry-Paraffin-NBP2-94325-img0002.jpg)
![Immunohistochemistry: PRPSAP2 Antibody [NBP3-35590] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using PRPSAP2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35590_rabbit-prpsap2-pab-912202414112813.jpg "Immunohistochemistry: PRPSAP2 Antibody [NBP3-35590] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using PRPSAP2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: PRPSAP2 Antibody [NBP3-35590] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using PRPSAP2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35590_rabbit-prpsap2-pab-9122024159524.jpg)
![Immunohistochemistry: PRPSAP2 Antibody [NBP3-35590] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PRPSAP2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35590_rabbit-prpsap2-pab-91220241519530.jpg)
![Western Blot: PRPSAP2 Antibody [NBP3-35590] - Western blot analysis of various lysates using PRPSAP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35590_rabbit-prpsap2-pab-912202415133482.jpg)
![Immunohistochemistry: PRPSAP2 Antibody [NBP3-35590] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using PRPSAP2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35590_rabbit-prpsap2-pab-91220241581242.jpg)
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202415143627.jpg "Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Human esophagus using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202414163033.jpg)
![Western Blot: RAC2 Antibody [NBP3-35170] - Western blot analysis of various lysates, using RAC2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-91220241519516.jpg)
![Immunohistochemistry: RAC2 Antibody [NBP3-35170] - Immunohistochemistry analysis of paraffin-embedded Mouse stomach using RAC2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35170_rabbit-rac2-pab-912202415121394.jpg)
![Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-1012202411392946.jpg "Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human stomach cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-1012202411392984.jpg)
![Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-1012202411381280.jpg)
![Immunocytochemistry/ Immunofluorescence: MAGEA1 Antibody [NBP3-38158] - Immunofluorescence analysis of HeLa cells using MAGEA1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-1012202411343363.jpg)
![Western Blot: MAGEA1 Antibody [NBP3-38158] - Western blot analysis of lysates from 22Rv1 cells, using MAGEA1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-101220241142108.jpg)
![Western Blot: ELF5 Antibody [NBP3-04468] - Analysis of extracts of various cell lines, using ELF5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ELF5-Antibody-Western-Blot-NBP3-04468-img0002.jpg "Western Blot: ELF5 Antibody [NBP3-04468] - Analysis of extracts of various cell lines, using ELF5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: ELF5 Antibody [NBP3-04468] - Analysis of A549 cells using ELF5 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ELF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04468-img0001.jpg)
![Immunohistochemistry-Paraffin: ELF5 Antibody [NBP3-04468] - Rat liver using ELF5 Rabbit pAb at dilution of 1:50 (40x lens).](http://images.novusbio.com/fullsize/ELF5-Antibody-Immunohistochemistry-Paraffin-NBP3-04468-img0004.jpg)
![Immunohistochemistry-Paraffin: ELF5 Antibody [NBP3-04468] - Mouse brain using ELF5 Rabbit pAb at dilution of 1:50 (40x lens).](http://images.novusbio.com/fullsize/ELF5-Antibody-Immunohistochemistry-Paraffin-NBP3-04468-img0003.jpg)
![Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145] - Western blot analysis of lysates from HeLa cells using Vinculin Rabbit mAb at 1:320000-1:1280000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16145_rabbit-vinculin-mab-9i3y8-5220251265322.jpg "Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145] - Western blot analysis of lysates from HeLa cells using Vinculin Rabbit mAb at 1:320000-1:1280000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (9I3Y8) [NBP3-16145] - Confocal imaging of paraffin-embedded Human colon cancer tissue using Vinculin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16145_rabbit-vinculin-mab-9i3y8-522025125423.jpg)
