Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35401-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38144-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-35293-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38540-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26918-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38239-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38178-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92968-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92722-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35581-20ul | |||
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat heart using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241519590.jpg "Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat heart using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241513346.jpg)
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Rat liver using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-91220241418155.jpg)
![Western Blot: HUS1 Antibody [NBP3-35401] - Western blot analysis of lysates from mouse kidney, using HUS1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-912202414213762.jpg)
![Immunohistochemistry: HUS1 Antibody [NBP3-35401] - Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using HUS1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35401_rabbit-hus1-pab-912202414163053.jpg)
![Western Blot: Cytochrome b5 type A Antibody [NBP3-38144] - Western blot analysis of various lysates using Cytochrome b5 type A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp3-38144_rabbit-cytochrome-b5-type-a-pab-91220241418197.jpg "Western Blot: Cytochrome b5 type A Antibody [NBP3-38144] - Western blot analysis of various lysates using Cytochrome b5 type A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.")
![Immunohistochemistry: Cytochrome b5 type A Antibody [NBP3-38144] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using Cytochrome b5 type A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38144_rabbit-cytochrome-b5-type-a-pab-912202414112862.jpg)
![Immunohistochemistry: Cytochrome b5 type A Antibody [NBP3-38144] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using Cytochrome b5 type A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38144_rabbit-cytochrome-b5-type-a-pab-912202415175229.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP3-38144] - Immunofluorescence analysis of C6 cells using Cytochrome b5 type A Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38144_rabbit-cytochrome-b5-type-a-pab-912202415111829.jpg)
![Western Blot: Insulin Antibody (4C3Y9) [NBP3-15643] - Western blot analysis of extracts of various cell lines, using Insulin antibody (NBP3-15643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Insulin-Antibody-4C3Y9-Western-Blot-NBP3-15643-img0005.jpg "Western Blot: Insulin Antibody (4C3Y9) [NBP3-15643] - Western blot analysis of extracts of various cell lines, using Insulin antibody (NBP3-15643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643] - Immunohistochemistry of paraffin-embedded rat pancreatic islet using Insulin Rabbit mAb (NBP3-15643) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Insulin-Antibody-4C3Y9-Immunohistochemistry-Paraffin-NBP3-15643-img0001.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643] - Immunohistochemistry of paraffin-embedded mouse pancreatic islet using Insulin Rabbit mAb (NBP3-15643) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Insulin-Antibody-4C3Y9-Immunohistochemistry-Paraffin-NBP3-15643-img0002.jpg)
![Immunohistochemistry: Insulin Antibody (4C3Y9) [NBP3-15643] -Confocal imaging of human pancreas using Insulin Rabbit mAb (at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.](http://images.novusbio.com/fullsize/nbp3-15643_rabbit-insulin-mab-4c3y9-immunohistochemistry-23102024105444..jpg)
![Immunohistochemistry: Septin-5 Antibody [NBP3-35293] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Septin-5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35293_rabbit-septin-5-pab-10122024122413.jpg "Immunohistochemistry: Septin-5 Antibody [NBP3-35293] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Septin-5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: Septin-5 Antibody [NBP3-35293] - Western blot analysis of various lysates, using Septin-5 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35293_rabbit-septin-5-pab-10122024125287.jpg)
![Immunohistochemistry: Septin-5 Antibody [NBP3-35293] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Septin-5 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35293_rabbit-septin-5-pab-101220241214467.jpg)
![Immunocytochemistry/ Immunofluorescence: Septin-5 Antibody [NBP3-35293] - Immunofluorescence analysis of U-2 OS cells using Septin-5 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35293_rabbit-septin-5-pab-1012202411531073.jpg)
![Immunohistochemistry: FECH Antibody [NBP3-38540] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using FECH Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38540_rabbit-fech-pab-101220241252842.jpg "Immunohistochemistry: FECH Antibody [NBP3-38540] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using FECH Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: FECH Antibody [NBP3-38540] - Immunohistochemistry analysis of paraffin-embedded Human stomach using FECH Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38540_rabbit-fech-pab-1012202411531026.jpg)
![Western Blot: FECH Antibody [NBP3-38540] - Western blot analysis of various lysates using FECH Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38540_rabbit-fech-pab-1012202411572626.jpg)
![Immunohistochemistry: FECH Antibody [NBP3-38540] - Immunohistochemistry analysis of paraffin-embedded Mouse stomach using FECH Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38540_rabbit-fech-pab-1012202411535840.jpg)
![Immunohistochemistry: FECH Antibody [NBP3-38540] - Immunohistochemistry analysis of paraffin-embedded Rat heart using FECH Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38540_rabbit-fech-pab-10122024121080.jpg)
![Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in COVID-19 Patient Lung. Analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S1 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161943.jpg "Immunohistochemistry: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in COVID-19 Patient Lung. Analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 Spike S1 antibody (0.5 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.")
![Western Blot: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation with SARS-CoV-2 Spike Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike S1, NBP3-26918, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 ug/mL and Lane 2: 2 ug/mL.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161944.jpg)
![ELISA: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation with SARS-CoV-2 Spike Recombinant Protein. Antibodies: SARS-CoV-2 Spike S1 antibody, NBP3-26918. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 Spike S1 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161940.jpg)
![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Validation of SARS-CoV-2 Spike S1 in 293 Transfected Cells. Analysis of 4% paraformaldehyde-fixed Spike transfected 293 cells labeling SARS-CoV-2 Spike S1 with NBP3-26918 at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161942.jpg)
![Western Blot: SARS-CoV-2 Spike S1 Antibody - BSA Free [NBP3-26918] - Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 ug per lane of 293 cell lysate. Antibodies: SARS-CoV-2 Spike S1, NBP3-26918 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: WT 293 cells and Lane 2: SARS-CoV-2 Spike overexpressed 293 cells.](http://images.novusbio.com/fullsize/nbp3-26918_rabbit-sars-cov-2-spike-s1-pab-bsa-free-242024161941.jpg)
![Western Blot: Thyroid receptor-interacting protein 12 Antibody [NBP2-81864] - Analysis of Thyroid receptor-interacting protein 12 in rat colon tissue lysate with Thyroid receptor-interacting protein 12 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Thyroid-receptor-interacting-protein-12-Antibody-Western-Blot-NBP2-81864-img0001.jpg "Western Blot: Thyroid receptor-interacting protein 12 Antibody [NBP2-81864] - Analysis of Thyroid receptor-interacting protein 12 in rat colon tissue lysate with Thyroid receptor-interacting protein 12 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: Thyroid receptor-interacting protein 12 Antibody - BSA Free [NBP2-81864] - Immunofluorescence of Thyroid receptor-interacting protein 12 in rat colon tissue with Thyroid receptor-interacting protein 12 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81864_rabbit-polyclonal-thyroid-receptor-interacting-protein-12-antibody-2711202419562160.jpg)
![Immunocytochemistry/ Immunofluorescence: Thyroid receptor-interacting protein 12 Antibody - BSA Free [NBP2-81864] - Immunocytochemistry of Thyroid receptor-interacting protein 12 in Hela cells with Thyroid receptor-interacting protein 12 Antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81864_rabbit-polyclonal-thyroid-receptor-interacting-protein-12-antibody-2711202420324860.gif)
![Immunohistochemistry: Thyroid receptor-interacting protein 12 Antibody - BSA Free [NBP2-81864] - Immunohistochemistry of Thyroid receptor-interacting protein 12 in rat colon tissue with Thyroid receptor-interacting protein 12 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81864_rabbit-polyclonal-thyroid-receptor-interacting-protein-12-antibody-2711202420285888.jpg)
![Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Human prostate using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241252820.jpg "Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Human prostate using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: Proenkephalin Antibody [NBP3-38239] - Immunofluorescence analysis of paraffin-embedded Human adrenal gland using Proenkephalin Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241252847.jpg)
![Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241224152.jpg)
![Western Blot: Proenkephalin Antibody [NBP3-38239] - Western blot analysis of lysates from THP-1 cells, using Proenkephalin Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-10122024126238.jpg)
![Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody [NBP3-38178] - Immunofluorescence analysis of U2OS cells using [KO Validated] Cdk5 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-101220241243970.jpg "Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody [NBP3-38178] - Immunofluorescence analysis of U2OS cells using [KO Validated] Cdk5 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: Cdk5 Antibody [NBP3-38178] - Western blot analysis of various lysates using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-1012202411572659.jpg)
![Western Blot: Cdk5 Antibody [NBP3-38178] - Western blot analysis of various lysates using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-101220241155289.jpg)
![Immunoprecipitation: Cdk5 Antibody [NBP3-38178] - Immunoprecipitation analysis of 900 ug extracts of A-549 cells using 3 ug Cdk5 antibody. Western blot was performed from the immunoprecipitate using Cdk5 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-1012202411552850.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk5 Antibody [NBP3-38178] - Western Blot analysis of lysates from wild type (WT) and Cdk5 knockout (KO) 293T cells, using [KO Validated] Cdk5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38178_rabbit-cdk5-pab-31120251744477.jpg)
![Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Western-Blot-NBP2-92968-img0005.jpg "Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% non](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Knockout-Validated-NBP2-92968-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody - BSA Free [NBP2-92968] - Immunofluorescence analysis of HeLa cells using [KO Validated] Casein Kinase 1 epsilon Rabbit pAb . Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92968_rabbit-polyclonal-casein-kinase-1-epsilon-antibody-422025143182.jpg%3Ft=1709606037)
![Western Blot: CABP7 Antibody [NBP2-92722] - Analysis of extracts of various cell lines, using CABP7 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CABP7-Antibody-Western-Blot-NBP2-92722-img0005.jpg "Western Blot: CABP7 Antibody [NBP2-92722] - Analysis of extracts of various cell lines, using CABP7 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: CABP7 Antibody [NBP2-92722] - Mouse testis using CABP7 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CABP7-Antibody-Immunohistochemistry-Paraffin-NBP2-92722-img0004.jpg)
![Immunohistochemistry-Paraffin: CABP7 Antibody [NBP2-92722] - Human breast cancer using CABP7 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CABP7-Antibody-Immunohistochemistry-Paraffin-NBP2-92722-img0001.jpg)
![Immunohistochemistry-Paraffin: CABP7 Antibody [NBP2-92722] - Human stomach using CABP7 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol..](http://images.novusbio.com/fullsize/CABP7-Antibody-Immunohistochemistry-Paraffin-NBP2-92722-img0002.jpg)
![Immunohistochemistry-Paraffin: CABP7 Antibody [NBP2-92722] - Mouse kidney using CABP7 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CABP7-Antibody-Immunohistochemistry-Paraffin-NBP2-92722-img0003.jpg)
![Immunohistochemistry: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Serpin A8/Angiotensinogen Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35581_rabbit-serpin-a8-angiotensinogen-pab-912202415111855.jpg "Immunohistochemistry: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Serpin A8/Angiotensinogen Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Immunohistochemistry analysis of paraffin-embedded Human placenta using Serpin A8/Angiotensinogen Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35581_rabbit-serpin-a8-angiotensinogen-pab-912202414163056.jpg)
![Western Blot: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Western blot analysis of various lysates using Serpin A8/Angiotensinogen Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35581_rabbit-serpin-a8-angiotensinogen-pab-912202414123818.jpg)
![Immunocytochemistry/ Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP3-35581] - Immunofluorescence analysis of HepG2 cells using Serpin A8/Angiotensinogen Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35581_rabbit-serpin-a8-angiotensinogen-pab-912202415121397.jpg)