Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38572-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38248-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-36704-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38311-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38365-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95177-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35454-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35658-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03599-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04690-20ul | |||
![Immunohistochemistry: ALDH7A1 Antibody [NBP3-38572] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ALDH7A1 Rabbit pAb at a dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38572_rabbit-aldh7a1-pab-101220241262313.jpg "Immunohistochemistry: ALDH7A1 Antibody [NBP3-38572] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ALDH7A1 Rabbit pAb at a dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: ALDH7A1 Antibody [NBP3-38572] - Western blot analysis of various lysates, using ALDH7A1 Rabbit pAb at 1:400 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Negative control (NC): K-562.Exposure time: 75s.](http://images.novusbio.com/fullsize/nbp3-38572_rabbit-aldh7a1-pab-1012202411535850.jpg)
![Immunohistochemistry: ALDH7A1 Antibody [NBP3-38572] - Immunohistochemistry analysis of ALDH7A1 in paraffin-embedded rat heart tissue using ALDH7A1 Rabbit pAb at a dilution of 1:50 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38572_rabbit-aldh7a1-pab-3112025173173.jpg%3Ft=1697185133)
![Immunohistochemistry: ALDH7A1 Antibody [NBP3-38572] - Immunohistochemistry analysis of ALDH7A1 in paraffin-embedded mouse colon tissue using ALDH7A1 Rabbit pAb at a dilution of 1:50 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38572_rabbit-aldh7a1-pab-3112025177021.jpg%3Ft=1697185133)
![Immunohistochemistry: ALDH7A1 Antibody [NBP3-38572] - Immunohistochemistry analysis of ALDH7A1 in paraffin-embedded human kidney tissue using ALDH7A1 Rabbit pAb at a dilution of 1:50 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38572_rabbit-aldh7a1-pab-3112025173132.jpg%3Ft=1697185133)
![Western Blot: RCC1 Antibody [NBP3-38248] - Western blot analysis of various lysates using RCC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38248_rabbit-rcc1-pab-101220241243932.jpg "Western Blot: RCC1 Antibody [NBP3-38248] - Western blot analysis of various lysates using RCC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunohistochemistry: RCC1 Antibody [NBP3-38248] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RCC1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38248_rabbit-rcc1-pab-101220241252839.jpg)
![Immunocytochemistry/ Immunofluorescence: RCC1 Antibody [NBP3-38248] - Immunofluorescence analysis of HeLa cells using RCC1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38248_rabbit-rcc1-pab-101220241233859.jpg)
![Immunohistochemistry: RCC1 Antibody [NBP3-38248] - Immunohistochemistry analysis of paraffin-embedded Rat lung using RCC1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38248_rabbit-rcc1-pab-101220241154554.jpg)
![Immunohistochemistry: RCC1 Antibody [NBP3-38248] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using RCC1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38248_rabbit-rcc1-pab-1012202411535869.jpg)
![Western Blot: FBXO21 Antibody [NBP3-36704] - Western blot analysis of various lysates using FBXO21 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-36704_rabbit-fbxo21-pab-1012202411392945.jpg "Western Blot: FBXO21 Antibody [NBP3-36704] - Western blot analysis of various lysates using FBXO21 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunohistochemistry: FBXO21 Antibody [NBP3-36704] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FBXO21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-36704_rabbit-fbxo21-pab-101220241142109.jpg)
![Immunohistochemistry: FBXO21 Antibody [NBP3-36704] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FBXO21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-36704_rabbit-fbxo21-pab-1012202411364634.jpg)
![Immunohistochemistry: FBXO21 Antibody [NBP3-36704] - Immunohistochemistry analysis of paraffin-embedded Rat lung using FBXO21 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-36704_rabbit-fbxo21-pab-1012202411343350.jpg)
![Immunocytochemistry/ Immunofluorescence: FBXO21 Antibody [NBP3-36704] - Immunofluorescence analysis of L929 cells using FBXO21 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-36704_rabbit-fbxo21-pab-101220241134339.jpg)
![Immunohistochemistry: PNK Antibody [NBP3-38311] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PNK Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38311_rabbit-pnk-pab-1012202411545543.jpg "Immunohistochemistry: PNK Antibody [NBP3-38311] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PNK Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: PNK Antibody [NBP3-38311] - Western blot analysis of various lysates using PNK Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38311_rabbit-pnk-pab-101220241233819.jpg)
![Immunocytochemistry/ Immunofluorescence: PNK Antibody [NBP3-38311] - Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNK Rabbit pAb. Green: GFP-RNF168 fusion protein expression for DNA damage marker.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.](http://images.novusbio.com/fullsize/nbp3-38311_rabbit-pnk-pab-1012202411535887.jpg)
![Immunohistochemistry: PNK Antibody [NBP3-38311] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using PNK Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38311_rabbit-pnk-pab-101220241233897.jpg)
![Immunohistochemistry: PNK Antibody [NBP3-38311] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using PNK Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38311_rabbit-pnk-pab-1012202411535892.jpg)
![Immunohistochemistry: EIF3C Antibody [NBP3-38365] - Immunohistochemistry analysis of paraffin-embedded Mouse intestine using EIF3C Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38365_rabbit-eif3c-pab-91220241581257.jpg "Immunohistochemistry: EIF3C Antibody [NBP3-38365] - Immunohistochemistry analysis of paraffin-embedded Mouse intestine using EIF3C Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: EIF3C Antibody [NBP3-38365] - Immunofluorescence analysis of U2OS cells using EIF3C Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38365_rabbit-eif3c-pab-912202415152897.jpg)
![Immunohistochemistry: EIF3C Antibody [NBP3-38365] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using EIF3C Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38365_rabbit-eif3c-pab-912202414213768.jpg)
![Western Blot: EIF3C Antibody [NBP3-38365] - Western blot analysis of various lysates using EIF3C Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38365_rabbit-eif3c-pab-912202414112824.jpg)
![Immunohistochemistry: EIF3C Antibody [NBP3-38365] - Immunohistochemistry analysis of paraffin-embedded Rat brain using EIF3C Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38365_rabbit-eif3c-pab-912202414213775.jpg)
![Western Blot: MTIF2 Antibody [NBP2-95177] - Analysis of extracts of various cell lines, using MTIF2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/MTIF2-Antibody-Western-Blot-NBP2-95177-img0006.jpg "Western Blot: MTIF2 Antibody [NBP2-95177] - Analysis of extracts of various cell lines, using MTIF2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")
![Immunohistochemistry-Paraffin: MTIF2 Antibody [NBP2-95177] - Paraffin-embedded human lung cancer using MTIF2 .](http://images.novusbio.com/fullsize/MTIF2-Antibody-Immunohistochemistry-Paraffin-NBP2-95177-img0005.jpg)
![Immunohistochemistry-Paraffin: MTIF2 Antibody [NBP2-95177] - Paraffin-embedded human colon carcinoma using MTIF2 .](http://images.novusbio.com/fullsize/MTIF2-Antibody-Immunohistochemistry-Paraffin-NBP2-95177-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody - Azide and BSA Free [MTIF2] - Immunofluorescence analysis of C6 cells using MTIF2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95177_rabbit-polyclonal-mtif2-antibody-52202516321412.jpg)
![Immunocytochemistry/ Immunofluorescence: MTIF2 Antibody - Azide and BSA Free [MTIF2] - Immunofluorescence analysis of L929 cells using MTIF2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95177_rabbit-polyclonal-mtif2-antibody-52202516293990.jpg)
![Western Blot: COX15 Antibody [NBP3-35454] - Western blot analysis of various lysates using COX15 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35454_rabbit-cox15-pab-1012202411353044.jpg "Western Blot: COX15 Antibody [NBP3-35454] - Western blot analysis of various lysates using COX15 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")
![Immunohistochemistry: COX15 Antibody [NBP3-35454] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using COX15 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35454_rabbit-cox15-pab-1012202411381213.jpg)
![Immunohistochemistry: COX15 Antibody [NBP3-35454] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using COX15 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35454_rabbit-cox15-pab-1012202411334060.jpg)
![Immunohistochemistry: COX15 Antibody [NBP3-35454] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using COX15 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35454_rabbit-cox15-pab-1012202411421086.jpg)
![Immunocytochemistry/ Immunofluorescence: COX15 Antibody [NBP3-35454] - Immunofluorescence analysis of L929 cells using COX15 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35454_rabbit-cox15-pab-1012202411381240.jpg)
![Western Blot: DDX6 Antibody [NBP3-35658] - Western blot analysis of various lysates using DDX6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202414163078.jpg "Western Blot: DDX6 Antibody [NBP3-35658] - Western blot analysis of various lysates using DDX6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202415143678.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NBP3-35658] - Immunofluorescence analysis of L929 cells using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-91220241519588.jpg)
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-912202415121317.jpg)
![Immunohistochemistry: DDX6 Antibody [NBP3-35658] - Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using DDX6 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35658_rabbit-ddx6-pab-9122024151959.jpg)
![Western Blot: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Analysis of extracts of various cell lines, using GPLD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/GPI-PLD-GPLD1-Antibody-Western-Blot-NBP3-03599-img0015.jpg "Western Blot: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Analysis of extracts of various cell lines, using GPLD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Rat kidney using GPI-PLD/GPLD1 Rabbit pAb (NBP3-03599) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GPI-PLD-GPLD1-Antibody-Immunohistochemistry-Paraffin-NBP3-03599-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Analysis of A-549 cells using GPLD1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/GPI-PLD-GPLD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03599-img0008.jpg)
![Immunohistochemistry-Paraffin: GPI-PLD/GPLD1 Antibody [NBP3-03599] - Rat brain using GPI-PLD/GPLD1 Rabbit pAb (NBP3-03599) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GPI-PLD-GPLD1-Antibody-Immunohistochemistry-Paraffin-NBP3-03599-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: GPI-PLD/GPLD1 Antibody - Azide and BSA Free [GPI-PLD/GPLD1] - Immunofluorescence analysis of HepG2 cells using GPI-PLD/GPLD1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03599_rabbit-polyclonal-gpi-pld-gpld1-antibody-5220251639617.jpg)
![Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-271120242074659.jpg "Immunocytochemistry/ Immunofluorescence: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling p16INK4a/CDKN2A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 10 ug of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0001.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: NBP3-06986 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0003.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody [NBP3-06986] - Loading: 15 ug of lysates per lane. Antibodies: CDKN2A (2 ug/mL), NBP3-06986 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-Western-Blot-NBP3-06986-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody - BSA Free [NBP3-06986] - Validation of p16INK4a/CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-p16INK4a/CDKN2A antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-06986_rabbit-p16ink4a-cdkn2a-pab-bsa-free-2711202420115536.gif)
![Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/KIF3A-Antibody-Western-Blot-NBP3-05030-img0003.jpg "Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: KIF3A Antibody [NBP3-05030] - Analysis of MCF7 cells using KIF3A antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05030-img0001.jpg)
![Immunoprecipitation: KIF3A Antibody [NBP3-05030] - Analysis of 200ug extracts of MCF-7 cells, using KIF3A antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunoprecipitation-NBP3-05030-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF3A Antibody [NBP3-05030] - Rat testis using KIF3A Rabbit pAb at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-05030_rabbit-polyclonal-kif3a-antibody-immunohistochemistry-paraffin-146202493146..jpg)
![Western Blot: GNB2 Antibody [NBP3-04690] - Analysis of extracts of various cell lines, using GNB2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/GNB2-Antibody-Western-Blot-NBP3-04690-img0001.jpg "Western Blot: GNB2 Antibody [NBP3-04690] - Analysis of extracts of various cell lines, using GNB2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunohistochemistry-Paraffin: GNB2 Antibody [NBP3-04690] - Mouse pancreas using GNB2 Rabbit pAb (NBP3-04690) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GNB2-Antibody-Immunohistochemistry-Paraffin-NBP3-04690-img0005.jpg)
![Immunohistochemistry-Paraffin: GNB2 Antibody [NBP3-04690] - Rat stomach using GNB2 Rabbit pAb (NBP3-04690) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GNB2-Antibody-Immunohistochemistry-Paraffin-NBP3-04690-img0003.jpg)
![Immunohistochemistry-Paraffin: GNB2 Antibody [NBP3-04690] - Mouse testis using GNB2 Rabbit pAb (NBP3-04690) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/GNB2-Antibody-Immunohistochemistry-Paraffin-NBP3-04690-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: GNB2 Antibody - BSA Free [GNB2] - Immunofluorescence analysis of L929 cells using GNB2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04690_rabbit-polyclonal-gnb2-antibody-5220251639616.jpg)