240-B) using Human Twist-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Twist-2 was detected in immersion fixed A549 human lung carcinoma cells treated with Recombinant Human TGF-beta 1 (Catalog # 240-B) using Human Twist-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6249-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52579-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56209-25ul | |||
![Western Blot: Twist-2 Antibody (3C8) [H00117581-M01] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M01), clone 3C8.Lane 1: TWIST2 transfected lysate(18.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Twist-2-Antibody-3C8-Western-Blot-H00117581-M01-img0008.jpg "Western Blot: Twist-2 Antibody (3C8) [H00117581-M01] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M01), clone 3C8.Lane 1: TWIST2 transfected lysate(18.1 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01] - Analysis of monoclonal antibody to TWIST2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Twist-2-Antibody-3C8-Immunohistochemistry-Paraffin-H00117581-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Twist-2 Antibody (3C8) [H00117581-M01] - Immunoperoxidase of monoclonal antibody to TWIST2 ( Cat # H00117581-M01 ) on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml]](http://images.novusbio.com/fullsize/Twist-2-Antibody-3C8-Immunohistochemistry-Paraffin-H00117581-M01-img0004.jpg)
![ELISA: Twist-2 Antibody (3C8) [H00117581-M01] - Detection limit for recombinant GST tagged TWIST2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Twist-2-Antibody-3C8-ELISA-H00117581-M01-img0009.jpg)
![Western Blot: Twist-2 Antibody (1E2) [H00117581-M06] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M06), clone 1E2. Lane 1: TWIST2 transfected lysatE (18.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Twist-2-Antibody-1E2-Western-Blot-H00117581-M06-img0003.jpg "Western Blot: Twist-2 Antibody (1E2) [H00117581-M06] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M06), clone 1E2. Lane 1: TWIST2 transfected lysatE (18.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Twist-2 Antibody (1E2) [H00117581-M06] - Analysis of monoclonal antibody to TWIST2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Twist-2-Antibody-1E2-Immunocytochemistry-Immunofluorescence-H00117581-M06-img0001.jpg)
![Immunoprecipitation: Twist-2 Antibody (1E2) [H00117581-M06] - Analysis of TWIST2 transfected lysate using anti-TWIST2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TWIST2 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Twist-2-Antibody-1E2-Immunoprecipitation-H00117581-M06-img0002.jpg)
![Western Blot: Twist-2 Antibody (1A11D9) [NBP2-52579] - Analysis using TWIST2 mAb against human TWIST2 (AA: 1-160) recombinant protein. (Expected MW is 44.1 kDa)](http://images.novusbio.com/fullsize/Twist-2-Antibody-1A11D9-Western-Blot-NBP2-52579-img0001.jpg "Western Blot: Twist-2 Antibody (1A11D9) [NBP2-52579] - Analysis using TWIST2 mAb against human TWIST2 (AA: 1-160) recombinant protein. (Expected MW is 44.1 kDa)")
![Western Blot: Twist-2 Antibody (1A11D9) [NBP2-52579] - Western blot aAagainst HEK293 (1) and TWIST2 (AA: 1-160)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Twist-2-Antibody-1A11D9-Western-Blot-NBP2-52579-img0002.jpg)
![ELISA: Twist-2 Antibody (1A11D9) [NBP2-52579] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/Twist-2-Antibody-1A11D9-ELISA-NBP2-52579-img0003.jpg)
![SDS-Page: Twist-2 Recombinant Protein [H00117581-P01]](http://images.novusbio.com/fullsize/Twist-2-Recombinant-Protein-SDS-Page-H00117581-P01-img0001.jpg "SDS-Page: Twist-2 Recombinant Protein [H00117581-P01]")
![Western Blot: Twist-2 Antibody (6H10) [NBP2-46255] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWIST2 .](http://images.novusbio.com/fullsize/Twist-2-Antibody-6H10-Western-Blot-NBP2-46255-img0001.jpg "Western Blot: Twist-2 Antibody (6H10) [NBP2-46255] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWIST2 .")
![Western Blot: Twist-2 Antibody (3C12) [H00117581-M12] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M12), clone 3C12. Lane 1: TWIST2 transfected lysatE (18.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Twist-2-Antibody-3C12-Western-Blot-H00117581-M12-img0002.jpg "Western Blot: Twist-2 Antibody (3C12) [H00117581-M12] - Analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M12), clone 3C12. Lane 1: TWIST2 transfected lysatE (18.1 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation: Twist-2 Antibody (3C12) [H00117581-M12] - Analysis of TWIST2 transfected lysate using anti-TWIST2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TWIST2 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Twist-2-Antibody-3C12-Immunoprecipitation-H00117581-M12-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Twist-2 Antibody [NBP2-56209] - Staining of human cell line BJ shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/Twist-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56209-img0001.jpg "Immunocytochemistry/Immunofluorescence: Twist-2 Antibody [NBP2-56209] - Staining of human cell line BJ shows localization to nucleus & nucleoli.")
![Western Blot: Twist2 Overexpression Lysate (Adult Normal) [NBL1-17460] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Twist2.](http://images.novusbio.com/fullsize/Twist2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17460-img0002.jpg "Western Blot: Twist2 Overexpression Lysate (Adult Normal) [NBL1-17460] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Twist2.")
