HAF007). A specific band was detected for Semaphorin 4A at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for Semaphorin 4A at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Semaphorin 4A Monoclonal Antibody (Catalog # MAB46941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Semaphorin 4A at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_lightbox"> MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4A Monoclonal Antibody (Catalog # MAB8166) or (B) Rat IgG₁Isotype Control (Catalog # MAB005) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />

HAF016). A specific band was detected for Semaphorin 4A at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for Semaphorin 4A at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Semaphorin 4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4694) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4A at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox"> MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="Human peripheral blood monocytes treated for 24 hours with 50 ng/mL PMA and 500 ng/mL of calcium ionomycin were stained with Mouse Anti-Human Semaphorin 4A Monoclonal Antibody (Catalog # MAB4694, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

2078-T4) that produces 50% of the optimal binding response is 0.100-0.600 μg/mL" class="big_lightbox"> 2078-T4) that produces 50% of the optimal binding response is 0.100-0.600 μg/mL" alt="When Recombinant Mouse Semaphorin 4A mFc Chimera Protein (Catalog #11096-S4) is immobilized at 2.0 µg/mL (100 µL/well), the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein (2078-T4) that produces 50% of the optimal binding response is 0.100-0.600 μg/mL" class="big_thumb" />