MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB356, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

DY990). Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> DY990). Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF017). A specific band was detected for HVEM/TNFRSF14 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for HVEM/TNFRSF14 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human thymus tissue and human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for HVEM/TNFRSF14 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB356) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> MAB356) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human PE-conjugated CD19 (FAB4867P). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human PE-conjugated CD19 (FAB4867P). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="Human PBMC lymphocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human PE-conjugated CD19 (FAB4867P). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V). Cells were gated on Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V). Cells were gated on Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Herpesvirus Entry Mediator (HVEM/TNFRSF14) was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3561) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." alt="Mouse splenocytes were stained with Rabbit Anti-Mouse HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB2516, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

9235-BT) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human HVEM/TNFRSF14 Fc Chimera Avi-tag binds with an ED₅₀ of 30.0-180 ng/mL." class="big_lightbox"> 9235-BT) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human HVEM/TNFRSF14 Fc Chimera Avi-tag binds with an ED₅₀ of 30.0-180 ng/mL." alt="When Recombinant Human BTLA (9235-BT) is immobilized at 1.00 µg/mL (100 µL/well), Biotinylated Recombinant Human HVEM/TNFRSF14 Fc Chimera Avi-tag binds with an ED₅₀ of 30.0-180 ng/mL." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="HVEM/TNFRSF14 was detected in immersion fixed paraffin-embedded sections of human appendix using Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3562) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />