HAF018). A specific band was detected for Glucokinase/GCK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Glucokinase/GCK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Huh-7 human hepatoma cell line, Hepa 1-6 mouse hepatoma cell line, and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glucokinase/GCK Monoclonal Antibody (Catalog # MAB7840) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glucokinase/GCK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />