NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD23/Fce RII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD23/Fce RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

123-FE) was serially diluted and captured by Mouse Anti-Human CD23/Fc epsilon  RII Monoclonal Antibody (Catalog # MAB1231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CD23/Fc epsilon  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 123-FE) was serially diluted and captured by Mouse Anti-Human CD23/Fc epsilon  RII Monoclonal Antibody (Catalog # MAB1231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CD23/Fc epsilon  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

FAB4867A) and either (A) Mouse Anti-Human CD23/Fce RII Monoclonal Antibody (Catalog # MAB123) or (B) Mouse IgG₁Isotype Control (MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> FAB4867A) and either (A) Mouse Anti-Human CD23/Fce RII Monoclonal Antibody (Catalog # MAB123) or (B) Mouse IgG₁Isotype Control (MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) and either (A) Mouse Anti-Human CD23/Fce RII Monoclonal Antibody (Catalog # MAB123) or (B) Mouse IgG₁Isotype Control (MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

123-FE) was serially diluted and captured by Mouse Anti-Human CD23/Fc epsilon  RII Monoclonal Antibody (Catalog # MAB1231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CD23/Fc epsilon  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 123-FE) was serially diluted and captured by Mouse Anti-Human CD23/Fc epsilon  RII Monoclonal Antibody (Catalog # MAB1231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CD23/Fc epsilon  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human CD23/Fc epsilon  RII (Catalog # 123-FE) was serially diluted and captured by Mouse Anti-Human CD23/Fc epsilon  RII Monoclonal Antibody (Catalog # MAB1231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CD23/Fc epsilon  RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF123) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." class="big_lightbox"> F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." alt="Mouse splenocytes were stained with Rat Anti-Mouse CD23/Fce RII Monoclonal Antibody (Catalog # MAB6900) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # MAB006)." class="big_thumb" />